Method for synthesizing hydrophis cyanocinctus antibacterial peptide Hc-CATH through multi-copy integration and high-density fermentation

By constructing two copies of plasmids in tandem and optimizing the fermentation process using Pichia cerevisiae, the problem of insufficient yield of the antimicrobial peptide Hc-CATH in the prior art was solved, efficient expression and purification were achieved, and industrial needs were met.

CN119661676APending Publication Date: 2025-03-21JIANGSU KANGJUN BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202311219114.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-09-21
Publication Date
2025-03-21

AI Technical Summary

Technical Problem

The prior art cannot meet the industrial needs of Qinghuanhai snake antibacterial peptide Hc-CATH, and its synthesis method is inefficient.

Method used

Through genetic engineering technology, two copies of plasmids were constructed, and Pichia cerevisiae was used as the host to optimize the fermentation process to achieve high-level fermentation and synthesis of the antimicrobial peptide Hc-CATH of the Qinghuanhai snake.

Benefits of technology

The yield of the antimicrobial peptide Hc-CATH of Qinghuanhai snake was improved, and efficient expression and purification were achieved. It has the advantages of strong versatility, high expression efficiency, good biological activity, low cost and easy to produce on a large scale.

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Abstract

The invention provides a method for synthesizing a hydrophis cyanocinctus antibacterial peptide Hc-CATH through multi-copy integration and high-density fermentation, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a series-connection two-copy plasmid is constructed, pichia pastoris is taken as a host, two-copy series-connection protein is successfully expressed, and the expression yield of the hydrophis cyanocinctus antibacterial peptide Hc-CATH is improved by 2-3 times. According to the present invention, the hydrophis cyanocinctus antibacterial peptide Hc-CATH can be synthesized at a high level through the construction and optimization of the two-copy integration method and the optimization of the fermentation process, and the method has advantages of strong versatility, high expression efficiency, biological activity, low cost, easy large-scale production, and the like, and can be used for the preparation of the hydrophis cyanocinctus antibacterial peptide Hc-CATH.
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