One-step method for synthesizing DNA functionalized Zn doped CdTe quantum dot

A functionalized quantum dot technology, applied in the field of nanomaterials and biomedical analytical chemistry, can solve the problems of high toxicity, unfavorable biomedical applications, and few products, and achieve the effect of simple preparation method, superior fluorescence performance, and low cost

Active Publication Date: 2013-02-27
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the quantum dot probe synthesized by this method has a low yield (15%), high toxicity, and few products, which is not conducive to biomedical applications.

Method used

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  • One-step method for synthesizing DNA functionalized Zn doped CdTe quantum dot
  • One-step method for synthesizing DNA functionalized Zn doped CdTe quantum dot
  • One-step method for synthesizing DNA functionalized Zn doped CdTe quantum dot

Examples

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Effect test

Embodiment 1

[0028] A method for synthesizing DNA functionalized Zn-doped CdTe quantum dots in one step, the steps are as follows:

[0029] 1. Add 0.8mmol NaBH 4 Put the solid and 0.2mmol Te powder into a 5mL flask, add 1mL ultrapure water, stir and react at 0°C for 5h to obtain a NaHTe solution, and set aside;

[0030] 2. Add 0.025mmol CdCl 2 , 0.025mmol ZnCl 2 Put 0.05mmol NAC and 0.05mmol NAC in a 10ml centrifuge tube, add 4mL ultrapure water to dissolve, then adjust the pH=8 with 1mol / L NaOH solution, pass argon gas for 1min to remove oxygen in the water, and inject 60 microliters of the obtained product in step 1 with a syringe NaHTe solution, add 40nmol DNA fragments, oscillate and mix, then transfer to a hydrothermal reactor and heat up to 200°C for 25min to obtain DNA functionalized CdTe:Zn 2+ Quantum dot solution;

[0031] The DNA fragment was designed by the inventor and then synthesized by Shanghai Sangong. The sequence is 5′-AAT ACC ACA TCATCC ATA TAA AAA TCG GGC GGA-3′, an...

Embodiment 2

[0034] A method for synthesizing DNA functionalized Zn-doped CdTe quantum dots in one step, the steps are as follows:

[0035] 1. Add 0.6mmol NaBH 4 Put the solid and 0.3mmol Te powder into a 5mL flask, add 1mL ultrapure water, and stir and react at 25°C for 2h to obtain a NaHTe solution, which is set aside;

[0036] 2. Add 0.025mmol CdCl 2 , 0.05mmol ZnCl 2 Put 0.075mol NAC and 0.075mol NAC in a 10ml centrifuge tube, add 4mL ultrapure water to dissolve, adjust pH=9 with 1mol / L NaOH solution, pass argon for 1min, inject 50 microliters of NaHTe solution obtained in step 1 with a syringe, add 120nmol DNA fragments, oscillated and mixed, then transferred to a hydrothermal reactor and heated to 180 ° C for 30 minutes to obtain DNA functionalized CdTe:Zn 2+ Quantum dot solution a;

[0037] The DNA fragment was designed by the inventor and then synthesized by Shanghai Sangong. The sequence is 5′-GAAGTGAAAATGACAGAACACAACAAAAA TCG GGC-3′, 6 bases at the 3′ end are modified by phos...

Embodiment 3

[0044] A method for synthesizing DNA functionalized Zn-doped CdTe quantum dots in one step, the steps are as follows:

[0045] 1. Add 1mmol KBH 4 Put the solid and 0.2mmol Te powder into a 5mL flask, add 1mL ultrapure water, and stir and react at 35°C for 1.5h to obtain a KHTe solution, which is set aside;

[0046] 2. Add 0.025mmol CdCl 2 , 0.075mmol ZnCl 2 Put 0.1mmol NAC and 0.1mmol NAC in a 10ml centrifuge tube, add 4mL ultrapure water to dissolve, adjust the pH=10 with 1mol / L NaOH solution, pass argon for 1min, inject 30 microliters of the KHTe solution obtained in step 1 with a syringe, add 80nmol DNA fragments, after shaking and mixing, transfer to a hydrothermal reactor and heat up to 160 ° C for 35 minutes to obtain DNA functionalized CdTe:Zn 2+ Quantum dot solution;

[0047] The DNA fragment was designed by the inventor and then synthesized by Shanghai Sangong. The sequence is 5′-GGTTGGTGTGGTTGGAAAAAAAATCG GGCGG-3′, and the 8 bases at the 3′ end are modified by ph...

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Abstract

The invention belongs to the fields of nanometer materials and biomedical analysis chemistry, and particularly relates to a one-step method for synthesizing DNA functionalized Zn doped CdTe quantum dots. The quantum dot probe is prepared by connecting phosphorylated DNA to surface of quantum dot by coordination of sulfur and cadmium, and quantum dot surface can be connected with 1-2 DNA fragments. The preparation method is as below: in a synthesis process of quantum dots, phosphorylated DNA is added to obtain the DNA functionalized quantum dot probe through a one-step reaction. The probe can be used as a bifunctional nanometer material, has high fluorescence quantum yield, good biocompatibility and good dispersion, and can be used for detection of DNA fragment and protein, cell identification and tumor targeting. Compared with a traditional DNA marked quantum dot method, the preparation method provided by the invention is simple and low-cost, does not require expensive preparation facilities, can be accomplished in general chemistry laboratory, and can be widely used in fields of biological detection, cellular imaging, target tracing and disease diagnosis, etc.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials and biomedical analytical chemistry, in particular to a method for synthesizing DNA functionalized Zn-doped CdTe quantum dots in one step. Background technique [0002] Quantum dots, also known as semiconductor nanocrystals, are zero-dimensional nanomaterials with a diameter of 1-10 nm. Due to their own quantum effects, quantum dots have size effects, surface effects, quantum tunneling effects and dielectric confinement effects. It is precisely because of its special physical effects that quantum dots have unparalleled fluorescent properties compared with commonly used fluorescent dyes: wide excitation spectrum and continuous distribution, good stability, high luminous efficiency, and strong photobleaching resistance. In recent years, due to their unique optical properties, quantum dots have been studied more and more in analytical chemistry, biological detection, cell imaging, in vivo tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88
Inventor 何治柯张翠玲许婧方旸
Owner WUHAN UNIV
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