Method for enhancing high GC content DNA fragment amplification efficiency by organic solvent
An organic solvent and amplification efficiency technology, applied in the field of molecular biology, can solve the problems of ineffectiveness and unclearness, and achieve the effect of convenient use, less side effects, and easy long-term storage at room temperature
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[0027] Below in conjunction with embodiment, the present invention is further described; Following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.
[0028] The optimization method of the PCR technology involved in the present invention is realized by adding an organic solvent in the PCR system, and its specific operation method is as follows:
[0029] 1. Extraction of human genomic DNA to be amplified:
[0030] 2. Configuration and optimization of PCR system
[0031] According to the existing technology, the PCR system is different according to the different DNA fragments to be amplified. Specifically, the amount of dNTP, template, Mg2+, and primer added is selected according to different templates and different lengths of amplified fragments;
[0032] H in the PCR reaction system 2 O is double distilled water and above grade water, the amount of water added in the system should be adjusted accordi...
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