Preparation method and use of biomimetic anti-caries polypeptide

Abstract

The invention discloses a biomimetic anti-caries polypeptide and its preparation method and use. The invention discloses the biomimetic anti-caries polypeptide and its salt or ester. The biomimetic anti-caries polypeptide has an amino acid sequence shown in the formula of SEQ ID NO.1 or SEQ ID NO.2. The biomimetic anti-caries polypeptide mainly contains the amino acid sequence HA6-1 (SVSVGMKPSPRP) having the HA combination feature and the amino acid sequence QPX inducing early-stage caries remineralization, and can also contain the amino acid sequence TKREEVD acting on calcium ions and causing the HA nucleation. The biomimetic anti-caries polypeptide has small molecular weight, can improve absorption on the surface of enamel, can exert its effect better, can promote remineralization of early demineralized enamel (early decayed tooth), can be synthesized conveniently, and has good economical efficiency, good effects and high safety and reliability.

Description

technical field [0001] The invention relates to an anti-caries technology, in particular to a bionic anti-caries polypeptide and its preparation method and application. Background technique [0002] Caries is the most common oral disease in humans. Due to the high incidence and wide prevalence of dental caries, which seriously affects oral and general health, the World Health Organization has listed it as one of the three major non-communicable diseases that human beings should prevent and treat, second only to cardiovascular diseases and tumors. [0003] As a classic anti-caries agent, fluoride has been used in various forms over the past forty years, such as fluoridated drinking water, fluoridated milk or fluoridated table salt, topical fluoride application (fluoride toothpaste, Fluoride-containing gel, fluoride-containing mouthwash), etc., which can reduce the caries rate in the population to varying degrees, and are recognized as the most effective caries prevention mea...

AI Technical Summary

Problems solved by technology

Among the above-mentioned sources of enamel matrix protein, the extraction method requires more tissue materials and obtains mixed components of different molecular weights, making it difficult to obtain high-purity products; the procedure of gene recombinant protein is complicated, and the post-translational processing and modification system is not perfect. It is very difficult, and the protein function is often imperfect; Swedish Emdogain?? is the purification of enamel matrix protein extracted from pig tooth germ, and the three relative molecular weight peaks of typical amelogenin are obtained by freeze-drying and preservation. Although it has obvious effect of promoting periodontal tissue regeneration, but Possible loss of effective anti-caries functional fragments

To sum up, the current method of obtaining enamel matrix protein is complex and may lose effective anti-caries fragments. How to use enamel matrix protein for effective caries prevention has become a difficult problem that has been wanted to be solved in this field but has not been solved.

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