The invention discloses a method for improving the
biomass and flavone content of a
saussurea involucrata
cell culture, which comprises the following steps of: culturing yellow-white
saussurea involucrata
callus on a culture medium at the temperature of 24 DEG C, illuminating for 16 hours / day, subculturing once every 15 days and subculturing for three times to obtain yellow-white or yellow-green
callus which grows vigorously and is compact in structure; the preparation method of the culture medium comprises the following steps: weighing MS,
cane sugar,
naphthylacetic acid, 6-benzyladenine,
coconut extract and
agar in a container, adding
distilled water, fully stirring, fixing the volume to 1 L, uniformly stirring, and adjusting the pH value to obtain a basic culture solution; carrying out high-pressure sterilization on the basic culture solution, pouring a flat plate, and cooling to obtain a culture medium; wherein the
coconut extract is one or two of coconut water or coconut milk. By adding coconut water or coconut milk, the
biomass of the
saussurea involucrata
callus can be remarkably increased in cooperation with the MS culture medium, and compared with an existing report, the increasing multiple is 2-3 times that of the existing report.