Method for producing fermented soybean meal by two-step inoculation segmented solid-state fermentation

Abstract

The invention provides a method for producing fermented soybean meal by two-step inoculation segmented solid-state fermentation. The method comprises the following steps of: inoculating the mixed bacterial solution obtained by mixing bacillus subtilis solution, candida mycoderma bacteria solution and lactobacillus solution in peeled soybean meal and performing mixed fermentation; and inoculating the lactobacillus solution in the fermented product, mixing, and performing anaerobic fermentation to obtain the fermented soybean meal. The method is capable of meeting the different needs of aerobic bacteria and anaerobic bacteria, can be used for preparing high-quality fermented soybean meal, and is free of chemical additives; due to the various digestive enzymes, organic acids, medium and small molecular proteins (functional peptides), amino acids, vitamins, probiotics and the like generated during the fermentation process, the nutritive value of the soybean meal is greatly increased; the prepared fermented soybean meal contains not less than 16% of soybean peptide, not more than 25mg / g of glycinin, not more than 20mg / g of beta-conglycinin, not less than 3.5% of lactic acid, and not less than 2*10<9> beneficial viable bacteria; and microbial source nutrients are beneficial to the maintenance of intestinal structure and can promote immunologic function and improve animal production performance.

Description

technical field [0001] The invention relates to a fermentation method, in particular to a method for producing fermented soybean meal by secondary inoculation and staged solid-state fermentation and a fermentation product. Background technique [0002] As the phenomenon of adding antibiotics to feed is very common at present, for safety reasons, many countries prohibit the use of meat and bone meal and other animal protein raw materials produced from leftovers of livestock products for feed. Therefore, from the perspective of safety and cost, plant-based protein As a substitute for animal protein raw materials has become a trend. However, in order to efficiently utilize plant-derived protein, it is necessary to solve the problems of anti-nutritional factors and palatability that affect digestion and absorption; at the same time, in the prevention and treatment of diarrhea in young poultry and livestock breeding, the use of antibiotics seriously threatens our food safety. Am...

AI Technical Summary

Problems solved by technology

[0004] However, in the solid-state fermentation process of traditional solid-state fermentation compound bacteria, simultaneous inoculation of multiple strains cannot meet the fermentation requirements of different bacteria at the same time, resulting in incomplete fermentation