Multi-bacterium microbial compound preparations for aquaculture and preparation method thereof

A compound preparation, aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that aquatic animals have no direct effect, and achieve the effect of promoting nutrient absorption and promoting recovery

Active Publication Date: 2011-02-16
山西博亚方舟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most microbial agents are only effective against aquaculture water, but have no direct effect on aquatic animals themselves

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method for microbial preparations for aquaculture, comprising the steps of:

[0030] (1) Preparation of seed culture solution:

[0031] Photosynthetic bacteria( Rhodoplanes elegans ) Seed culture solution preparation of ACCC 10649: photosynthetic bacteria ( Rhodoplanes elegans ) ACCC 10649 is inoculated into a liquid medium under sterile conditions. The content of each component in 1.0L of distilled water in the liquid medium is: 3.0g of yeast powder, 3.0g of peptone, and 0.05g of magnesium sulfate heptahydrate , calcium chloride 0.3g, adjust the pH value to between 6.08 and 7.0, place it in a conventional light incubator, and culture it under conventional light at 28°C to 30°C for 6 to 7 days, and obtain the number of photosynthetic bacteria ≥ 5.0×10 9 cfu / ml seed culture solution.

[0032] Yeast ( Pichia fermentans lodder) ACCC 20319 T Preparation of the seed culture solution: the yeast ( Pichia fermentans lodder) ACCC 20319 T Inoculated into li...

Embodiment 2

[0039] (1) Preparation of seed culture solution:

[0040] Adopt the method of present embodiment 1, make seed culture liquid;

[0041] (2) Preparation of microbial preparations for aquaculture:

[0042] The seed culture solution of each bacterial species prepared in step (1) was prepared according to the photosynthetic bacteria ( Rhodoplanes elegans ) Seed culture solution of ACCC 10649, yeast ( Pichia fermentans lodder) ACCC 20319 T seed culture solution, Bacillus natto ( Bacillus natto Swamura ) seed culture solution of ACCC10614, Bacillus subtilis ( Bacillus subtills subsp. spizienii Nakamura et al) ACCC 10242 T seed culture solution, lactic acid bacteria ( Lactococcus lactis ) ACCC 11092 seed culture fluid and bifidobacteria ( Bifidobacterium sp) The weight percent of ACCC 11054 seed culture solution is: photosynthetic bacteria seed culture solution 35%, yeast seed culture solution 15%, Bacillus natto seed culture solution 25%, Bacillus subtilis seed cultu...

Embodiment 3

[0045] (1) Preparation of seed culture solution:

[0046] Adopt the method of present embodiment 1, make seed culture liquid;

[0047] (2) Preparation of microbial preparations for aquaculture:

[0048] The seed culture solution of each bacterial species prepared in step (1) was prepared according to the photosynthetic bacteria ( Rhodoplanes elegans ) Seed culture solution of ACCC 10649, yeast ( Pichia fermentans lodder) ACCC 20319 T seed culture solution, Bacillus natto ( Bacillus natto Swamura ) seed culture solution of ACCC10614, Bacillus subtilis ( Bacillus subtills subsp. spizienii Nakamura et al) ACCC 10242 T seed culture solution, lactic acid bacteria ( Lactococcus lactis ) ACCC 11092 seed culture fluid and bifidobacteria ( Bifidobacterium sp) The weight percentage of ACCC 11054 seed culture solution is: photosynthetic bacteria seed culture solution 30%, yeast seed culture solution 10%, Bacillus natto seed culture solution 20%, Bacillus subtilis seed cu...

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PUM

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Abstract

The invention relates to multi-bacterium microbial compound preparations for aquaculture and a preparation method thereof. The multi-bacterium microbial compound preparations are characterized by comprising the following steps of: preparing seed culture solution by performing single strain culture on rhodoplanes elegans, pichia fermentans lodder, bacillus natto swamura, bacillus subtills, lactococcus lactis and bifidobacterium sp respectively; inoculating the seed culture solution onto the same culture medium; and then culturing the seed culture solution by a fermentation tank, and naturally reducing a pH value of the seed culture solution to 3.0 to 3.5 to obtain the multi-bacterium microbial compound preparations, wherein the bacterial content of the pichia fermentans lodder in the preparations is more than or equal to 0.1*10<9>cfu / ml; the bacterial content of the other strains is more than or equal to 5.0*10<9>cfu / ml. The multi-bacterium microbial compound preparations for aquaculture can improve the ecological environment of aquatic animals, prevents and treats diseases, reduces the emergency condition of the animals, improves the quality of the aquatic animals, and shortens a listed period.

Description

technical field [0001] The invention belongs to the field of aquaculture, in particular to a multi-species microbial compound preparation for aquaculture and a preparation method thereof. Background technique [0002] With the emergence of a large number of large-scale aquaculture, the breeding density continues to increase, the excrement in the aquaculture water and the residue of the bait continue to increase, the pathogenic microorganisms multiply, the eutrophication of the aquaculture water is intensified, and the ecological environment is seriously damaged. , which has led to large-scale and frequent occurrence of various diseases and emergencies in aquatic animals. At the same time, the long-term use and abuse of antibiotics and fungicides has further led to the residues of various drugs in the aquaculture water and the resistance and resistance of pathogenic microorganisms. The improvement of the property and the further damage to the ecological environment of the aqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/46C12N1/20C12N1/00C12R1/07C12R1/84A23K1/16C12R1/01C12N1/16C12R1/125A23K10/18A23K50/80
CPCY02A40/818
Inventor 唐清池司金安王学宾尹宝华李忠宏胡伟郭银保陆勤王照范道佳李旭光白帅王建程刘志强郭金保
Owner 山西博亚方舟生物科技有限公司
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