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Lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals and preparation process thereof

A semi-solid fermentation and animal growth technology, applied in animal feed, animal feed, microorganism-based methods, etc., can solve the problems of short shelf life, color improvement, and more sedimentation, and achieve cost savings and significant feeding effects.

Active Publication Date: 2012-07-25
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Domestic and foreign studies have proved that liquid fermented feed (FLF) has better feeding performance than acidulant, and has been widely used abroad. However, in China, due to the low degree of industrialization of breeding, high cost, inconvenient transportation, and short shelf life, etc. Has been restricting the wide application of liquid fermented feed
At present, the milk enzyme products produced by insoluble medium still have problems of more precipitation and color improvement

Method used

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  • Lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals and preparation process thereof
  • Lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals and preparation process thereof
  • Lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals and preparation process thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Screening of bio-cola formula and process

[0046] 1 Materials and methods

[0047] 1.1 Raw materials: bran, soybean meal, corn flour, glucose, brown sugar, whey powder, yeast extract.

[0048] 1.2 Strains: Lactobacillus plantarum (Lactobacillus plantarum) LP bacteria powder 4.0×10 10 cfu / g, Pediococcus pentosaceus (Pediococcus pentosaceus) PP bacteria powder 2.9×10 11cfu / g (it was deposited in China Center for Type Culture Collection on February 22, 2012, and its preservation number is CCTCC NO: M 2012029).

[0049] 1.3 Optimization of the medium: Screen the basic medium, adjust the formula on this basis, and design an orthogonal experiment to optimize the medium formula.

[0050] 1.3.1 Basic formula 1: bran, soybean meal;

[0051] 1.3.2 Basic formula 2: corn flour, soybean meal, glucose;

[0052] 1.3.3 Basic formula 3: brown sugar, corn flour, inorganic salt, etc.

[0053] 1.3.4 Orthogonal test design: see Table 1.

[0054] Table 1 Optimum formula ...

Embodiment 2

[0115] Embodiment 2 quality improvement test

[0116] 1 Materials and methods

[0117] 1.1 Improvement of smell

[0118] Preliminary test results show that the lactic acid bacteria fermentation broth containing corn flour and brown sugar formula has a better smell. Considering that it has a certain improvement effect on the smell, the following groups are designed (Table 10) in order to improve the smell of the product.

[0119] Table 10 Odor-improving formula

[0120]

[0121] After anaerobic cultivation at 37°C for 24 hours, the pH of the fermentation broth and the number of viable lactic acid bacteria were measured and the odor was recorded.

[0122] 1.2 Determination of the amount of caramel color added

[0123] According to the suggestion of product addition, set 0.75‰, 1.0‰ and 1.25‰, record the color and status.

[0124] 1.3 Determination of the amount of stabilizer added

[0125] Ordinary suspending agent purchased from Hubei Huzhou Yuhe Food Additive Company. ...

Embodiment 3

[0152] Embodiment 3 Concrete fermentation process

[0153] 1. According to the recipe requirements, add the following ingredients: bran 4.5g, soybean meal 1.5g, glucose 1g, caramel color 0.1g, stabilizer 0.55g, add water to 100mL; if a higher number of viable lactic acid bacteria is required, it is recommended to use the formula: bran Skin 4.5g, soybean meal 1.5g, glucose 1g, whey powder 1g, caramel color 0.1g, stabilizer 0.55g, add water to 100mL.

[0154] 2. In the above formula medium, according to 1.3×10 7 The inoculation amount of cfu / 100mL is inoculated with lactic acid bacteria, among which the lactic acid bacteria are Pediococcus pentosaces (P.pentosaces) and Lactobacillus plantarum (L.plantarum), and the ratio of the number of viable bacteria between the two is 3:10;

[0155] 3. The above mixed solution is anaerobically cultured at 30° C. for 24 hours in FLF equipment (utility model patent application number 2011204617708), and the pH reaches 3.8, which is the end po...

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Abstract

The invention discloses a lactic acid bacteria semi-solid fermentation product. The product is prepared by the following processes: inoculating lactic acid bacteria in raw materials and fermenting at a temperature of 24-44 DEG C for 24-40h to obtain the product. The raw materials comprise 4.0-5.0g of wheat bran, 1.2-1.8g of soybean meal, 0.8-1.2g of glucose or brown sugar, and water, wherein water is added until the total amount of the raw materials is up to 100mL. The inoculation amount of the lactic acid bacteria is 1.0-2.0*10<7>cfu / 100mL, wherein the lactic acid bacteria is composed of Lactobacillus plantarum and Pediococcus pentosaceus with a strain viable count ratio of (3:10)-(10:3). The lactic acid bacteria semi-solid fermentation product for increasing growth performance of animals provided by the invention is the first fluid fermentation feed independently developed in China according to the national conditions for culture, inherits the advantages of the traditional solid fermentation feeds, and at the same time has the advantages of being more convenient, fresher, more significant in feeding effects, lower in cost and the like.

Description

technical field [0001] The invention relates to a lactic acid bacteria semi-solid fermentation product and process for improving animal growth performance. Background technique [0002] The production and application of fermented feed is a milestone for the aquaculture industry. Through the fermentation of beneficial bacteria, it can pre-digest the raw material, which can not only effectively improve the digestion and utilization rate of the feed, but also reduce the ammonia nitrogen content in the feces. Improving the breeding environment can also degrade the mycotoxins and anti-nutritional factors in the feed, and a large number of beneficial bacteria contained in the fermented feed can effectively adjust the balance of the intestinal flora of animals and improve the body's immunity. In addition, the fermented feed itself has good palatability and can Improve animal feed intake and promote animal growth. For the aquaculture industry that is severely polluted by antibiotic...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14C12N1/20C12R1/01
Inventor 单宝龙谷巍孙合美翟延庆徐海燕刘凤芝林显华李春凤汪官保刘红莲
Owner 山东宝来利来生物工程股份有限公司
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