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Comprehensive utilization process for marine fish skins

A technology of fish skin and fish, which is applied in the field of preparation of marine active substances, can solve the problems of low extraction efficiency of collagen or chondroitin sulfate, lack of comprehensive utilization of marine fish skin, complicated process, etc., and achieve simple and easy process, The effect of high extraction efficiency and increasing economic benefits

Active Publication Date: 2012-09-19
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a process for comprehensive utilization of marine fish skin to extract collagen and dermatan sulfate, to solve the problem that the prior art does not comprehensively utilize marine fish skin, and the extraction efficiency of collagen or chondroitin sulfate is not high , the process is more complex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Fish skin treatment: Rinse the fresh anglerfish skin with clean water, rinse it twice with distilled water, mash it and set aside;

[0032] (2) Fish skin impurity removal protein: Step (1) The mashed fish skin was mixed with NaOH solution with a concentration of 0.1 mol / L at a weight ratio of 1:10, stirred with a magnetic stirrer for 24 hours, and the NaOH solution was replaced every 6 hours during the period, and washed with distilled water until neutral;

[0033] (3) Remove fat from fish skin: Add 10% butanol solution to the fish skin treated in step (2) at a weight ratio of 1:15, stir and soak for 18 hours, rinse with distilled water to remove butanol;

[0034] (4) Fish skin enzymatic hydrolysis: Add distilled water to the fish skin obtained in step (3) at a weight ratio of 1:10, adjust the pH to 7.0, add 1% neutral protease by weight of the fish skin, keep stirring at 45°C for 5 hours, and then adjust with a concentration of 2mol / L NaOH solution When the ...

Embodiment 2

[0042] (1) Fish skin treatment: Rinse the fresh cod skin with clean water, then rinse it with distilled water 3 times, mash it and set aside;

[0043] (2) Fish skin impurity removal protein: Step (1) Mix the mashed fish skin with NaOH solution with a concentration of 0.15mol / L at a weight ratio of 1:15, stir with a magnetic stirrer for 36 h, change the NaOH solution once every 6 h during this period, and wash with distilled water until neutral ;

[0044] (3) Remove fat from fish skin: Add the butanol solution with a mass concentration of 15% to the fish skin obtained in step (2) at a weight ratio of 1:20, stir and soak for 24 hours, rinse with distilled water to remove butanol;

[0045] (4) Fish skin enzymatic hydrolysis: Add distilled water to the fish skin obtained in step (3) at a weight ratio of 1:15, adjust the pH to 7.5, add 2% neutral protease by weight of the fish skin, keep stirring at 55°C for 3 hours, and then use a concentration of 2.5mol / L NaOH solution Ad...

Embodiment 3

[0053] (1) Fish skin treatment: Rinse the frozen tuna skin with clean water, rinse it with distilled water 3 times, mash it and set aside;

[0054] (2) Fish skin impurity removal protein: Step (1) Mix the mashed fish skin with NaOH solution with a concentration of 0.12mol / L at a weight ratio of 1:12, stir with a magnetic stirrer for 30 h, change the NaOH solution once every 6 h during this period, wash with distilled water until neutral ;

[0055] (3) Remove fat from fish skin: Add the butanol solution with a mass concentration of 12% to the fish skin obtained in step (2) at a weight ratio of 1:18, stir and soak for 20 h, rinse with distilled water to remove butanol;

[0056] (4) Fish skin enzymatic hydrolysis: Add distilled water to the fish skin obtained in step (3) at a weight ratio of 1:12, adjust the pH to 7.2, add 1.5% neutral protease by weight of the fish skin, keep stirring at 50°C for 4 hours, and then use a concentration of 2.2mol / L NaOH solution Adjust the ...

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Abstract

The invention relates to the field of preparation of marine active substance from byproducts processed by aquatic animals and aims at providing a process for extracting collagen and dermatan sulfate by comprehensively utilizing marine fish skins. The process comprises the steps of fish skin treatment, removal of impure protein of the fish skin, removal of fat of the fish skin, fish skin enzymolysis, collagen salting, collagen collection, collagen dialysis and lyophilization, dermatan sulfate glucosidic bond protection, dermatan sulfate precipitation, dermatan sulfate purification and the like. According to the process disclosed by the invention, the active substances can be extracted from the marine fish skins simply, quickly and efficiently, the aim of cogenerating the collagen with the dermatan sulfate is achieved, the utilization benefits of the fish skins are increased and economic benefits are increased. The process is simple and feasible, high in extraction efficiency and suitable for industrial production.

Description

technical field [0001] The invention relates to the field of preparation of marine active substances derived from aquatic animal processing by-products, in particular to a process for comprehensively utilizing marine fish skin to extract collagen and dermatan sulfate. Background technique [0002] Collagen is the most abundant protein in animal sources, accounting for about 30% of the total animal protein. Widely present in the skin, bone, cartilage, ligament, tendon, blood vessel, cornea and other organs of vertebrates. Collagen is widely used in leather, cosmetics, food, membrane industry, pharmaceutical industry, biomedical materials, etc. [0003] Dermatan sulfate (detmatan sulfate, DS) is a mucopolysaccharide compound, which is the most important glycosaminoglycan in animal skin. It exists widely in animal tissues, especially in cell epidermis and extracellular matrix. It participates in cardiovascular Various pathological and physiological processes such as disease, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K14/78C08B37/08
Inventor 付万冬廖妙飞钟明杰杨会成郑斌周宇芳
Owner ZHEJIANG MARINE DEV RES INST
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