Marine fish mitochondrial genome control area amplification primer, as well as design and amplification methods

Abstract

The invention belongs to the field of research of marine fish mitochondrial genome control areas, and relates to an amplification primer of the marine fish mitochondrial genome control area. The amplification primer is composed of two chains of single-chain oligonucleotide, wherein a light-chain primer is a nucleotide sequence shown in SEQ ID No.1 and a heavy-chain primer is a nucleotide sequenceshown in SEQ ID No.2. The invention also provides a design method of the amplification primer and a method for amplifying the marine fish mitochondrial genome control area utilizing the amplificationprimer. The amplification primer provided by the invention can efficiently and specifically amplify the marine fish mitochondrial genome control area, and can be applicable to large-scale investigation and analysis of fish seed resources, thereby providing a powerful tool for rapidly and efficiently obtaining the marine fish mitochondrial genome control area sequence to carry out variety identification, seed resource investigation and control area evolution research.

Description

technical field [0001] The invention belongs to the research field of the mitochondrial genome control region of marine fish, and relates to a pair of PCR amplification primers (Marinefish-Dloop), in particular to an amplification primer capable of efficiently and specifically amplifying the mitochondrial genome control region of most marine fishes and Design method and amplification method. Background technique [0002] Animal mitochondrial genomic DNA is covalently closed circular, including a light chain and a heavy chain, and is a genetic factor with autonomous replication, transcription and translation capabilities outside the nucleus. Compared with nuclear DNA, animal mitochondrial genomic DNA has the characteristics of high copy number, fast primary structure evolution, and strict maternal inheritance, so it has become a powerful tool for aquaculture, fishery genetics research and evolutionary biology research. Mitochondrial genome consists of 37 coding genes and a m...

AI Technical Summary

Problems solved by technology

However, the selection of the design template sequence, the different design methods, and the limitations of the amplification ability of the universal primers themselves, the use of the reported universal primers cannot effectively amplify most marine fishes, mainly cichlids, so the design of a new The universal primers for the control region of , which are complementary to the reported primers, are necessary to effectively obtain the sequences of the control region of most fish mitochondria

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