Marine fish mitochondrial genome control area amplification primer, as well as design and amplification methods

A mitochondrial genome and amplification primer technology, applied in DNA preparation, recombinant DNA technology, DNA/RNA fragments, etc., can solve the problems of ineffective amplification of most marine fish and different amplification capabilities, and achieve strong amplification Abilities, effects of wide expansion range
CN102409051AInactive Publication Date: 2012-04-11ZHEJIANG OCEAN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG OCEAN UNIV
Publication Date
2012-04-11
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention belongs to the field of research of marine fish mitochondrial genome control areas, and relates to an amplification primer of the marine fish mitochondrial genome control area. The amplification primer is composed of two chains of single-chain oligonucleotide, wherein a light-chain primer is a nucleotide sequence shown in SEQ ID No.1 and a heavy-chain primer is a nucleotide sequenceshown in SEQ ID No.2. The invention also provides a design method of the amplification primer and a method for amplifying the marine fish mitochondrial genome control area utilizing the amplificationprimer. The amplification primer provided by the invention can efficiently and specifically amplify the marine fish mitochondrial genome control area, and can be applicable to large-scale investigation and analysis of fish seed resources, thereby providing a powerful tool for rapidly and efficiently obtaining the marine fish mitochondrial genome control area sequence to carry out variety identification, seed resource investigation and control area evolution research.
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Description

technical field

[0001] The invention belongs to the research field of the mitochondrial genome control region of marine fish, and relates to a pair of PCR amplification primers (Marinefish-Dloop), in particular to an amplification primer capable of efficiently and specifically amplifying the mitochondrial genome control region of most marine fishes and Design method and amplification method. Background technique

[0002] Animal mitochondrial genomic DNA is covalently closed circular, including a light chain and a heavy chain, and is a genetic factor with autonomous replication, transcription and translation capabilities outside the nucleus. Compared with nuclear DNA, animal mitochondrial genomic DNA has the characteristics of high copy number, fast primary structure evolution, and strict maternal inheritance, so it has become a powerful tool for aquaculture, fishery genetics research and evolutionary biology research. Mitochondrial genome consists of 37 coding genes and a m...

Claims

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