Low-value marine fish peptide meal as well as preparation method and application thereof
A low-value marine and peptide powder technology, applied in application, animal feed, additional food elements, etc., can solve the problems of low industrial concentration, poor production technology and product quality, small production scale, etc., to improve functional characteristics, The enzymatic hydrolysis process is easy to control and the reaction conditions are mild.
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Embodiment 1
[0026] Use degreased marine fish meal as raw material, mix evenly with water according to 1:10 ratio of material to water, at 121°C, 1.05kg / cm 2 Cook under pressure for 25 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 9, use alkaline protease Alcalase 2.4L (Novozymes, Denmark) as the enzymatic enzyme, control the concentration ratio of enzyme and substrate to 1%, carry out enzymatic hydrolysis at 60°C, and keep it in the enzyme During the hydrolysis process, the pH is stable. After the enzymolysis is completed, the temperature is raised to 92° C., inactivated for 10 minutes, and the filtrate is vacuum concentrated at a concentration temperature of 60° C. and a concentration pressure of 0.1 MPa, and finally dried to obtain the product.
[0027] After testing, the oligopeptide content in the low-value marine fish peptide powder prepared in this example is 30.80%, which is a high-quality low-value marine fish peptide powder.
Embodiment 2
[0029] Use degreased marine fish meal as raw material, mix evenly with water according to the material-water ratio of 1:5, at 115°C, 0.75kg / cm 2 Cook under pressure for 20 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 7.0, use flavor protease (Novozymes, Denmark) as the enzyme for enzymolysis, control the concentration ratio of enzyme and substrate to 0.5%, carry out enzymolysis at 45°C, and keep the pH during the enzymolysis process Stable, after enzymatic hydrolysis, the temperature is raised to 90°C, inactivated for 10 minutes, and the filtrate is vacuum-concentrated at a concentration temperature of 65°C and a concentration pressure of 0.12 MPa, and finally dried to obtain the product.
[0030] After testing, the oligopeptide content in the low-value marine fish peptide powder prepared in this example is 28.00%, which is a high-quality low-value marine fish peptide powder.
Embodiment 3
[0032] Use degreased marine fish meal as raw material, mix evenly with water according to 1: 12.5 ratio of material to water, at 130°C, 1.75kg / cm 2 Cook under pressure for 30 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 9.5, use alkaline protease (Wuxi Genencor Bioengineering Co., Ltd.) The pH is stable during the process. After the enzymolysis is completed, the temperature is raised to 95°C, inactivated for 10 minutes, and the filtrate is vacuum-concentrated at a concentration temperature of 65°C and a concentration pressure of 0.12 MPa, and finally dried to obtain the product.
[0033] After testing, the oligopeptide content in the low-value marine fish peptide powder prepared in this example is 25.27%, which is a high-quality low-value marine fish peptide powder.
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