Low-value marine fish peptide meal as well as preparation method and application thereof

A low-value marine and peptide powder technology, applied in application, animal feed, additional food elements, etc., can solve the problems of low industrial concentration, poor production technology and product quality, small production scale, etc., to improve functional characteristics, The enzymatic hydrolysis process is easy to control and the reaction conditions are mild.

Active Publication Date: 2014-10-08
连云港海娃食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although it is a big producer of fishmeal, it is difficult to call it a strong producer. There are many small regional enterprises in my country, the production scale is generally small, and the industrial concentration is low, resulting in poor production technology and product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use degreased marine fish meal as raw material, mix evenly with water according to 1:10 ratio of material to water, at 121°C, 1.05kg / cm 2 Cook under pressure for 25 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 9, use alkaline protease Alcalase 2.4L (Novozymes, Denmark) as the enzymatic enzyme, control the concentration ratio of enzyme and substrate to 1%, carry out enzymatic hydrolysis at 60°C, and keep it in the enzyme During the hydrolysis process, the pH is stable. After the enzymolysis is completed, the temperature is raised to 92° C., inactivated for 10 minutes, and the filtrate is vacuum concentrated at a concentration temperature of 60° C. and a concentration pressure of 0.1 MPa, and finally dried to obtain the product.

[0027] After testing, the oligopeptide content in the low-value marine fish peptide powder prepared in this example is 30.80%, which is a high-quality low-value marine fish peptide powder.

Embodiment 2

[0029] Use degreased marine fish meal as raw material, mix evenly with water according to the material-water ratio of 1:5, at 115°C, 0.75kg / cm 2 Cook under pressure for 20 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 7.0, use flavor protease (Novozymes, Denmark) as the enzyme for enzymolysis, control the concentration ratio of enzyme and substrate to 0.5%, carry out enzymolysis at 45°C, and keep the pH during the enzymolysis process Stable, after enzymatic hydrolysis, the temperature is raised to 90°C, inactivated for 10 minutes, and the filtrate is vacuum-concentrated at a concentration temperature of 65°C and a concentration pressure of 0.12 MPa, and finally dried to obtain the product.

[0030] After testing, the oligopeptide content in the low-value marine fish peptide powder prepared in this example is 28.00%, which is a high-quality low-value marine fish peptide powder.

Embodiment 3

[0032] Use degreased marine fish meal as raw material, mix evenly with water according to 1: 12.5 ratio of material to water, at 130°C, 1.75kg / cm 2 Cook under pressure for 30 minutes, take out and cool down to 60°C. Adjust the pH value of the cooking liquid to 9.5, use alkaline protease (Wuxi Genencor Bioengineering Co., Ltd.) The pH is stable during the process. After the enzymolysis is completed, the temperature is raised to 95°C, inactivated for 10 minutes, and the filtrate is vacuum-concentrated at a concentration temperature of 65°C and a concentration pressure of 0.12 MPa, and finally dried to obtain the product.

[0033] After testing, the oligopeptide content in the low-value marine fish peptide powder prepared in this example is 25.27%, which is a high-quality low-value marine fish peptide powder.

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PUM

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Abstract

The invention provides a method for preparing fish meal protein peptide. The method comprises the following steps: (1) taking defatted marine fish meal as a raw material, and adding water for dissolving according to the weight-bulk ratio of material to liquid being (1: 5)-(12.5); (2) under the temperature of 115-130 DEG C and pressure of 0.75-1.75 kg/cm<2>, cooking for 20-30 minutes; cooling to obtain cooking liquid; (3) adjusting the pH value of the cooking liquid to 7.0-9.5; adding protease, controlling the mass concentration ratio of protease to substrate to be 0.5-2.5 percent, and carrying out enzymolysis under the temperature of 45-70 DEG C to obtain enzymatic hydrolysate; and (4) heating the enzymatic hydrolysate to the temperature of 90-95 DEG C, inactivating and drying. The fish meal protein peptide is high in small-molecule oligopeptide content and digestibility. The fish meal protein peptide as a feed additive is matched with feed to be eaten by animals, so that the bioavailability of fish meal protein can be improved, the animal immunity also can be improved, and the disease resistance is enhanced.

Description

technical field [0001] The invention belongs to the technical field of marine fish deep processing, and in particular relates to a preparation method of low-value marine fish peptide powder. Background technique [0002] Fishmeal is a high-protein powder obtained from one or more types of marine fish, especially small non-edible fish as raw materials, after a series of processes such as cooking, pressing, dehydration and crushing. Fishmeal is the most commonly used protein source in aquafeeds due to its high quality. Fishmeal has become the most commonly used and most important animal in aquatic feed due to its rich content of essential amino acids, balanced amino acid composition, high protein content, good palatability, high digestibility, high unsaturated fatty acids and low anti-nutritional factors. sex protein source. Protein is one of the most critical nutrients in animal growth. The protein obtained by animals from food is decomposed into amino acids under the acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/04A23J3/34A23K1/16A23K1/10A23K10/22A23K20/147
Inventor 王立梅沈芳齐斌朱益波
Owner 连云港海娃食品有限公司
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