Convenient sex estimation method for juvenile flounder

A technology for juvenile fish sex and judging method, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of many operation steps, health hazards of operators, and many manpower and material resources, and achieve simple process, less time-consuming, The effect of low cost

Inactive Publication Date: 2010-08-18
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many steps in the tissue sectioning technique, such as a large number of judgments will inevitably cost more manpower and material resources, and the chemical substances such as xylene in the operation process have greater harm to the health of the operators

Method used

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  • Convenient sex estimation method for juvenile flounder
  • Convenient sex estimation method for juvenile flounder
  • Convenient sex estimation method for juvenile flounder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Take fresh juvenile flounder, weigh (full length 6.9cm, 2.78g), dissect and fix the abdomen containing gonad tissue in Davison's AFA fixative (absolute ethanol, formaldehyde, glacial acetic acid according to the volume ratio of 6 : 3: 1 preparation), after 24 hours of fixation, use 70% ethanol for long-term maintenance;

[0022] (2) Get the fixed flounder belly and place it on a glass slide, place it under a dissecting microscope, peel off its epidermis and muscle tissue, and take out the complete gonad tissue;

[0023] (3) Place the end of the gonad close to the kidney in the center of the field of view of the dissecting microscope, and gently smooth the epidermis with a dissecting needle and tweezers;

[0024] (4) Observe the shape of the gonad tissue in the epidermis of the gonad near the end of the kidney, and determine its gender according to the shape; the gonad is elongated and elongated in a "D" shape, and its sex is determined to be male. The gonad tissue ...

Embodiment 2

[0026] (1) Take fresh flounder juveniles, weigh them (full length 7.5cm, 3.12g), dissect and fix the abdomen containing gonad tissue in Davison's AFA fixative (absolute ethanol, formaldehyde, glacial acetic acid according to the volume ratio of 6 : 3: 1 preparation), after 24 hours of fixation, use 70% ethanol for long-term maintenance;

[0027] (2) Get the fixed flounder belly and place it on a glass slide, place it under a dissecting microscope, peel off its epidermis and muscle tissue, and take out the complete gonad tissue;

[0028] (3) Place the end of the gonad close to the kidney in the center of the field of view of the dissecting microscope, and gently smooth the epidermis with a dissecting needle and tweezers;

[0029] (4) Observe the shape of the gonad tissue in the epidermis of the gonad near the end of the kidney, and judge its gender according to the difference in shape; the gonad is larger and has a "heart" shape, and its sex is judged as female.

[0030] The g...

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Abstract

The invention relates to a convenient sex estimation method for a juvenile flounder, which comprises the following steps: (1) weighing a juvenile flounder, fixing the part of the abdomen including the gonad in a Davision's AFA fixative, processing for 24 hours, and preserving in ethanol of which the concentration is 70%; (2) placing the fixed abdomen of the juvenile flounder under the anatomical lens, peeling off the epidermis and muscle, and taking off the gonadal tissue; and (3) placing the gonadal tissue under the anatomical lens, and observing the shape of the gonadal tissue included in the gonad epidermis, wherein the gonadal tissue of a female flounder is bigger and is in the shape of a heart, while the gonadal tissue of a male flounder is smaller and slender and is in the shape of a lengthened D. The invention has the advantages of convenient and simple operation, low cost, and stable and reliable result, and is applicable to the researches on the propagation and the growth of multiple fishes, such as flounders and the like.

Description

technical field [0001] The invention belongs to the field of judging the sex of flounder fish, in particular to a simple method for judging the sex of flounder juveniles. Background technique [0002] Gender determination is the basis for the study of fish reproduction and development. The gonads of fish are divided into testis and ovary, which have different functions in fish breeding. In fish research, including fish reproductive development, endocrine regulation, external environment and chemical substances, etc., the reproductive and physiological effects of fish need to be determined. Flounder such as flounder has the characteristics that female individuals grow faster than male individuals. In order to improve production efficiency, parthenogenesis techniques such as gynogenesis, hormone-induced feminization, and temperature sex determination have been gradually developed. In these studies, it is necessary to determine the sex of juvenile fish and calculate the sex ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 孙鹏彭士明尹飞施兆鸿
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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