Preparation method of high-purity 95 isomaltose hypgather

A high-purity isomaltooligosaccharide technology, which is applied in the field of preparation of high-purity 95 isomaltooligosaccharides, can solve the problems of limited application range and low physiological function characteristics, and achieve low syrup viscosity, strong physiological function characteristics, and sweetness high effect

Active Publication Date: 2014-03-26
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two kinds of isomaltooligosaccharides that are common in the domestic market, 50-type isomalto-oligosaccharide and 90-type isomalto-oligosaccharide, of which the content of "three sugars" in the 50-type product is only 35%, containing a considerable amount of glucose and maltose and polysaccharides, which

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Using cornstarch as raw material, adjust the Baume degree to 12°Bé with tap water, adjust the pH value to 5.5, add high-temperature amylase at 0.6Kg / ton of starch, and adopt the secondary injection liquefaction technology. The temperature of the first injection is 105°C, and the second The injection temperature is 130°C, the temperature of the liquefied liquid is lowered to 55°C, and the pH value is adjusted to 5.8. Add α-fungal amylase at an amount of 0.8Kg / ton of starch, add transglycosidase at an amount of 0.7Kg / ton of starch, and the saccharification is completed Finally, the high-performance liquid chromatography was used to detect the components, including "trisaccharides" 35.5%, glucose 30%, maltose 7.5%, maltotriose 1.5%, tetrasaccharides and polysaccharides 25.5%.

[0026] After saccharification, the 50-type isomaltose oligosaccharide crude sugar solution was obtained, and the pH value was adjusted to 4.6. A powerful liquid glucoamylase was added at an amount of...

Embodiment 2

[0035] Using cornstarch milk as raw material, adjust the Baume degree to 22°Bé with tap water, adjust the pH value to 6.5, add high-temperature amylase at 0.8Kg / ton of starch, adopt the secondary injection liquefaction technology, the temperature of the first injection is 102°C, and the second The temperature of the first injection is 135°C, the temperature of the liquefied liquid is lowered to 60°C, the pH value is adjusted to 5.0, α-fungal amylase is added at an amount of 0.9Kg / ton of starch, and transglycosidase is added at an amount of 0.7Kg / ton of starch, and saccharified After the end, the high-performance liquid chromatography was used to detect the components, including "trisaccharides" 36.2%, glucose 29%, maltose 6.8%, maltotriose 1.2%, tetrasaccharides and polysaccharides 26.8%.

[0036] After saccharification, the 50-type isomaltose oligosaccharide crude sugar solution was obtained, and the pH value was adjusted to 4.8. A powerful liquid glucoamylase was added at an ...

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PUM

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Abstract

The invention provides a preparation method of high-purity 95 isomaltose hypgather. The preparation method comprises the following steps: performing jet liquefying on starch or starch milk used as a raw material; performing saccharification transglycosidation through maltase (fungus alpha-amylase or beta-amylase) and alpha-galctosyl-hydroxylysyl glucosyl transferase to obtain 50 type coarse isomaltose hypgather liquor; decomposing non-trisaccharide (isomaltose, panose and isomaltotriose hereinafter referred to as 'trisaccharide') ingredients by use of efficient liquid saccharifying enzyme to obtain glucose; separating by use of a chromatographic separation purification technology to remove glucose; decolorizing, desalting and refining to obtain 95 isomaltose hypgather (the 'trisaccharide' content is not less than 95%). The high-purity 95 isomaltose hypgather has the advantages that the effective ingredients 'trisaccharide' are high in purity reaching more than 95%, low in syrup viscosity and high in sweetness; the physiology functions are high; the bifidobacterium cultivation effect is three times higher than that of the common 90 type isomaltose hypgather.

Description

technical field [0001] The invention belongs to the technical field of functional oligosaccharide preparation, and in particular relates to a preparation method of high-purity 95 isomaltooligosaccharide. Background technique [0002] Isomaltooligosaccharide, also known as branched oligosaccharide, is a functional oligosaccharide made from starch through glycosylation and transglycoside conversion with biological enzymes. Isomaltooligosaccharide has good moisture retention and can inhibit the retrogradation and aging of starch and the precipitation of crystalline sugar in food; the "trisaccharide" in isomaltooligosaccharide (refers to isomaltose + panose + isomaltotriose, the same below) It has good proliferative activity of bifidobacteria, can inhibit the growth of harmful bacteria, can effectively prevent constipation, promote the absorption of minerals such as calcium and magnesium, and has low dental caries. It is widely used in food, beverages, and health products. It is...

Claims

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Application Information

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IPC IPC(8): C12P19/20C12P19/18C12P19/14C12P19/22
Inventor 干昭波邵先豹窦宝德窦光朋张安国贾玉秋
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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