Dual-miRNA-initiated master key-drug delivery system and construction method and application thereof

Abstract

The invention discloses a dual-miRNA-initiated master key-drug delivery system and a construction method and application thereof. The dual-miRNA-initiated master key-drug delivery system comprises a gold nanocage, a fluorescent chemotherapy drug and a DNA bridge-type nanolock, wherein the fluorescent chemotherapy drug is loaded in a hole cavity of the gold nanocage, the DNA bridge-type nanolock is combined with the surface of the gold nanocage to block the hole cavity of the gold nanocage, the DNA bridge-type nanolock is composed of SH-modified long-chain DNA S1, SH-modified long-chain DNA S2, long-chain DNA S3 with a composition sequence of an Mg <2+> enzyme structure and long-chain DNA S4 with an Mg<2+> enzyme recognition sequence, the S1 and the S2 are combined with the surface of the gold nanocage through gold-sulfur bonds, the S3 and the S1 are in partial-base-complementary connection, and the two ends of the S4 are respectively in base-complementary connection with free ends of the S1 and the S2. The function of unlocking a plurality of locks can be executed by a master key by utilizing a cell endogenous substance Mg<2+>-dependent DNA enzyme cyclic shearing reaction, and a drug is released in cancer cells in a targeted and enhanced manner, so that the signal-amplification diagnosis and treatment function of a probe is realized.

Description

technical field [0001] The invention belongs to the technical field of tumor diagnosis and treatment, and in particular relates to a dual miRNA-induced master key-drug delivery system and its construction method. Background technique [0002] In recent years, with the emergence and rapid development of nanotechnology, a series of functionalized nanomaterials with special physical and chemical properties have been continuously developed. Nanoprobes and nanomedicines have a huge specific surface area and can controllably modify a variety of ligands. Molecules, through ligand-receptor interactions, efficiently cross barriers in the body and specifically bind to diseased cells. Nanocarriers can simultaneously load imaging molecules and therapeutic drugs, and observe the distribution and metabolism of drugs in the body through real-time imaging to achieve precise imaging-guided treat. At the same time, as functionalized nucleic acid materials, nucleic acid aptamers and DNA enzym...

AI Technical Summary

Problems solved by technology

[0009] In view of this, the present invention aims to propose a double miRNA as a master key to initiate, and the endogenous substance Mg2+ of the cell is assisted by the enzyme The drug-released gold nanocage drug-loading system is used for cancer treatment combined with tumor-targeted therapy and photothermal therapy to solve the problems of poor precision, poor effect and large side effects of traditional chemotherapy methods

Images