Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dual-miRNA-initiated master key-drug delivery system and construction method and application thereof

A master key and delivery system technology, applied in the field of tumor diagnosis and treatment, can solve the problems of large side effects, poor effect, poor precision, etc., and achieve the effect of reducing toxic side effects and simple probe preparation method

Active Publication Date: 2021-12-10
QINGDAO UNIV OF SCI & TECH
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of this, the present invention aims to propose a double miRNA as a master key to initiate, and the endogenous substance Mg2+ of the cell is assisted by the enzyme The drug-released gold nanocage drug-loading system is used for cancer treatment combined with tumor-targeted therapy and photothermal therapy to solve the problems of poor precision, poor effect and large side effects of traditional chemotherapy methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dual-miRNA-initiated master key-drug delivery system and construction method and application thereof
  • Dual-miRNA-initiated master key-drug delivery system and construction method and application thereof
  • Dual-miRNA-initiated master key-drug delivery system and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Design DNA bridging nano-lock probes and the DNA sequences involved. The specific sequence information is shown in the table below.

[0046]

[0047] 2. The configuration of phosphate buffer saline (PBS)

[0048] Accurately weigh 0.3581g Na 2 HPO 4 2H 2 O and 0.1560 g NaH 2 PO 4 2H 2 O, respectively dissolved and then fixed to volume to obtain a salt solution with a concentration of 0.01M. After the pH meter is calibrated, pour the disodium salt solution into the beaker and insert the electrode, add the dihydrogen sodium salt solution, stir and mix evenly in small amounts for several times, and the pH meter stabilizes to 7.40, that is, the PBS buffer solution, which is placed in a brown jar, Store at 4°C.

[0049] 3. Construction of gold nanocage drug loading system AuNCs / DNA@Dox

[0050] 3.1 Preparation of gold nanocages (AuNCs)

[0051] (1) Use an electronic balance to accurately weigh 0.03 g of polyvinylpyrrolidone (PVP), dissolve it in 1.5 mL of solve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a dual-miRNA-initiated master key-drug delivery system and a construction method and application thereof. The dual-miRNA-initiated master key-drug delivery system comprises a gold nanocage, a fluorescent chemotherapy drug and a DNA bridge-type nanolock, wherein the fluorescent chemotherapy drug is loaded in a hole cavity of the gold nanocage, the DNA bridge-type nanolock is combined with the surface of the gold nanocage to block the hole cavity of the gold nanocage, the DNA bridge-type nanolock is composed of SH-modified long-chain DNA S1, SH-modified long-chain DNA S2, long-chain DNA S3 with a composition sequence of an Mg <2+> enzyme structure and long-chain DNA S4 with an Mg<2+> enzyme recognition sequence, the S1 and the S2 are combined with the surface of the gold nanocage through gold-sulfur bonds, the S3 and the S1 are in partial-base-complementary connection, and the two ends of the S4 are respectively in base-complementary connection with free ends of the S1 and the S2. The function of unlocking a plurality of locks can be executed by a master key by utilizing a cell endogenous substance Mg<2+>-dependent DNA enzyme cyclic shearing reaction, and a drug is released in cancer cells in a targeted and enhanced manner, so that the signal-amplification diagnosis and treatment function of a probe is realized.

Description

technical field [0001] The invention belongs to the technical field of tumor diagnosis and treatment, and in particular relates to a dual miRNA-induced master key-drug delivery system and its construction method. Background technique [0002] In recent years, with the emergence and rapid development of nanotechnology, a series of functionalized nanomaterials with special physical and chemical properties have been continuously developed. Nanoprobes and nanomedicines have a huge specific surface area and can controllably modify a variety of ligands. Molecules, through ligand-receptor interactions, efficiently cross barriers in the body and specifically bind to diseased cells. Nanocarriers can simultaneously load imaging molecules and therapeutic drugs, and observe the distribution and metabolism of drugs in the body through real-time imaging to achieve precise imaging-guided treat. At the same time, as functionalized nucleic acid materials, nucleic acid aptamers and DNA enzym...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/54A61K49/00A61K41/00A61K31/704A61P35/00
CPCA61K47/6949A61K47/549A61K41/0052A61K31/704A61K49/0021A61K49/0093A61K49/0052A61P35/00A61K2300/00
Inventor 叶素娟马龙飞李荣华张纪华
Owner QINGDAO UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com