Homogeneous compound catalyst/enzyme structure, fabricating method thereof and application thereof

Abstract

The invention provides a homogeneous compound catalyst / enzyme structure, a fabricating method thereof and an application thereof. According to the homogeneous compound catalyst / enzyme structure and the fabricating method thereof, an electrophoretic deposition (EPD) is employed. On the proper electrophoretic deposition condition, a plurality of catalyst particles and enzyme molecules are deposited and fixed simultaneously on a substrate, and thus a compound catalyst / enzyme film is form on the surface of the substrate. The compound catalyst / enzyme film contains a plurality of enzyme molecules, which is mixed and distributed homogeneously and used for catalyzing biomolecule reactions, as well as catalyst materials used for catalyzing electrochemical reactions. The compound structure can be used to form a working electrode of a biological recognition element of a microsensor.

Description

technical field [0001] The present invention relates to a catalyst / enzyme structure and its preparation method, especially to a compound catalyst / enzyme structure with a uniform structure and its preparation method and application. This method can be applied to quite a wide range of fields, and it can It is applied to the substrate immobilization of organic molecular detectors or biosensors (such as micro biosensors), etc. Background technique [0002] Please refer to figure 1 , which shows a schematic diagram of a basic structure of a biosensor. The basic structure of the biosensor is mainly composed of three parts: a biological recognition element (Bioreceptor) 11 , a signal transducer (Signal transducer) 12 and a signal processor (Signal processor) 13 . Its sensing principle is: when the biosensing element 11 with biochemical specificity combines or reacts with the substance 15 to be measured, the physical or chemical changes produced by it can be converted into Signif...

AI Technical Summary

Problems solved by technology

The shortcoming of traditionally prepared miniature biosensors is: the structure on the surface of the working electrode (comprising substrate 21 and silver wire 22) made by this method belongs to the structure of catalyst / enzyme layered accumulation: its inner layer is catalyst layer 24, the outer layer is the enzyme layer 26

like figure 2 The layered structure shown may hinder the diffusion of hydrogen peroxide to the catalyst layer 24 along with the thickness of the enzyme layer 26, and make the surface pass through the hydrogen peroxide produced by glucose oxidase, because this layer of thick film causes the diffusion on the electrode surface resistance to mass transfer and leads to a decrease in sensing capability

In particular, it will cause the sensitivity (Sensitivity) of the sensor to decrease in a high-concentration glucose environment.

[0021] Furthermore, the preparation of micro-biosensors by traditional methods requires a considerable number of procedures, and the catalyst materials and enzyme molecules are raw materials with high unit prices. The traditional procedures for manufacturing micro-biosensors often require excessive amounts of catalyst materials and enzyme molecules. Achieving a predetermined sensing capability without reducing the production cost of miniature biosensors

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