Application of L-threonine transaldolase to synthesis of florfenicol chiral intermediates

A technology of threonine and transaldolase, which is applied in the fields of biotechnology and chemical engineering, can solve the problems of cumbersome production steps, large environmental pollution, and high production costs, and achieve the effects of short reaction time, reduced production costs, and mild reaction conditions
CN110540977AActive Publication Date: 2019-12-06福建昌生生物科技发展有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
福建昌生生物科技发展有限公司
Publication Date
2019-12-06

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Abstract

The invention provides application of L-threonine transaldolase to synthesis of florfenicol chiral intermediates. The L-threonine transaldolase is selected from any one of the following groups that (1) polypeptide contains an amino acid sequence shown in SEQ ID NO:1; (2) polypeptide contains an amino acid sequence greater than or equal to 90% homology shown in SEQ ID NO:1, and the polypeptide hascatalytic activity; and (3) derived polypeptide is formed by substitution, deletion or addition of 1-5 amino acid residues to the amino acid sequence shown in SEQ ID NO:1 and with the retention of catalytic activity. According to the application, new L-threonine transaldolase genes are screened through gene mining, the L-threonine transaldolase derived from recombinant escherichia coli is expressed by adopting a genetic engineering method, methylsulfonyl benzaldehyde and the L-threonine transaldolase are used as raw materials, (2S,3R)-methylsulfonyl phenylserine is synthesized through a wholecell catalyzed reaction, fluorfenicol key chiral synthesis blocks can be obtained by a one-step reaction under the room temperature and pressure, environmental protection is achieved, and the application is an environment-friendly biosynthetic pathway.
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Description

technical field

[0001] The invention belongs to the fields of biotechnology and chemical engineering, and in particular relates to the application of L-threonine transaldolase in the synthesis of florfenicol chiral intermediates. Background technique

[0002] (2S,3R)-P-thymphenylphenylserine is the key chiral building block for the synthesis of Florfenicol. Florfenicol is a β-aminoalcohol antibiotic. It has the advantages of rapid absorption, wide distribution in the body, long half-life, no aplastic anemia side effects, no drug resistance, no residue, and no cross-resistance. It is an alternative to chloramphenicol. and thiamphenicol, a new generation of chloramphenicol antibiotics. It is mainly used to treat bacterial diseases and mycoplasma infections in pigs, chickens and fish caused by sensitive bacteria, and has unique curative effects on diseases caused by Escherichia coli in livestock and poultry, porcine infectious pleuropneumonia and panting disease. In recent ye...

Claims

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