Quick identification of powdery mildew resistant gene for barley

Abstract

The invention provides quick identification of a powdery mildew resistant gene for barley, relates to subject knowledge of plant comparative genomics, genetics, bioinformatics and the like and belongs to the field of plant biotechnology science. The quick identification mainly comprises the following steps: (1) downloading a barley whole-genome sequence and acquiring an MLO gene; (2) identifying the MLO gene; (3) establishing a phylogenetic relationship of the MLO gene; and (4) comparing an MLO powdery mildew gene. According to the quick identification, the mining period of the barley powdery mildew gene is effectively shortened to facilitate quick identification of the powdery mildew gene; the corresponding coseparation functional markers (SNP, SCAR and the like) are developed through the identified candidate powdery mildew gene and quickly used for molecular marker auxiliary selection of the powdery mildew resistant gene, and the accuracy is high; through combination with other anti-disease gene molecular markers, creation of a multi-resistant breeding material can be carried out, the breeding period is shortened, and the breeding efficiency is improved; and a foundation is laid for stating the barley powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the barley powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics, and candidate gene strategies by means of sequencing the whole genome sequence of barley, and mainly involves the download of the whole genome sequence of barley and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Barley belongs to the cereal family Poaceae. It has a nutty aroma, is high in carbohydrates, and moderate in protein, calcium, and phosphorus. In North Africa and parts of Asia, barley is one of the staple foods. In addition, the barley straw is soft, and it is mostly used as grass for livestock, and it is also used as roughage in large quantities. Barley powdery mildew occurs mainly in humid and semi-humid areas. It can...

AI Technical Summary

Problems solved by technology

Barley powdery mildew can cause yield losses of more than 20%

Usually, the prevention and control of diseases mainly depends on spraying fungicides, but long-term use of drugs will lead to environmental pollution and variation of physiological races of pathogens, so promoting disease-resistant varieties is the safest and most effective method

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