Quick identification of powdery mildew resistant gene for barley

A powdery mildew-resistant gene and powdery mildew-resistant technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve problems such as yield loss, bacterial race variation, environmental pollution, etc., and shorten the breeding period. , the effect of shortening time and low efficiency

Inactive Publication Date: 2015-04-29
CHANGSHU CITY DONGBANG TOWN LIMU VEGETABLE PROFESSION COOP
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Barley powdery mildew can cause yield losses of more than 20%
Usually, the prevention and control of diseases mainly depends on spraying fungicides, but long-term use of dr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quick identification of powdery mildew resistant gene for barley
  • Quick identification of powdery mildew resistant gene for barley
  • Quick identification of powdery mildew resistant gene for barley

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can rapidly identify barley powdery mildew gene. The specific implementation process is as follows:

[0032] 1) Collection and identification of barley MLO-type genes

[0033] In order to obtain all the members of the MLO gene family in barley, we first constructed an HMM model based on the MLO genes of Arabidopsis thaliana and the MLO gene sequences of resistance to powdery mildew in tomato, pea, pepper, rose, pepper, and lotus root. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383,TC285032...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides quick identification of a powdery mildew resistant gene for barley, relates to subject knowledge of plant comparative genomics, genetics, bioinformatics and the like and belongs to the field of plant biotechnology science. The quick identification mainly comprises the following steps: (1) downloading a barley whole-genome sequence and acquiring an MLO gene; (2) identifying the MLO gene; (3) establishing a phylogenetic relationship of the MLO gene; and (4) comparing an MLO powdery mildew gene. According to the quick identification, the mining period of the barley powdery mildew gene is effectively shortened to facilitate quick identification of the powdery mildew gene; the corresponding coseparation functional markers (SNP, SCAR and the like) are developed through the identified candidate powdery mildew gene and quickly used for molecular marker auxiliary selection of the powdery mildew resistant gene, and the accuracy is high; through combination with other anti-disease gene molecular markers, creation of a multi-resistant breeding material can be carried out, the breeding period is shortened, and the breeding efficiency is improved; and a foundation is laid for stating the barley powdery mildew resistance molecular mechanism.

Description

technical field [0001] The present invention is to quickly identify the barley powdery mildew gene by using methods such as plant comparative genomics, genetics, bioinformatics, and candidate gene strategies by means of sequencing the whole genome sequence of barley, and mainly involves the download of the whole genome sequence of barley and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] Barley belongs to the cereal family Poaceae. It has a nutty aroma, is high in carbohydrates, and moderate in protein, calcium, and phosphorus. In North Africa and parts of Asia, barley is one of the staple foods. In addition, the barley straw is soft, and it is mostly used as grass for livestock, and it is also used as roughage in large quantities. Barley powdery mildew occurs mainly in humid and semi-humid areas. It can...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/29C12Q1/68
Inventor 钱春桃其他发明人请求不公开姓名
Owner CHANGSHU CITY DONGBANG TOWN LIMU VEGETABLE PROFESSION COOP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap