Breeding method of new Berkshire pig disease-resistant variety

A breeding method and technology for Berkshire pigs, applied in the field of animal genetics, breeding and reproduction, and molecular biology, can solve problems such as the conception rate of sows, the decrease in the survival rate of weaned piglets born alive, and the decrease in disease resistance and fecundity , to achieve the effects of increasing annual genetic progress, reducing morbidity, and high lean meat rate

Inactive Publication Date: 2017-12-22
YANGZHOU UNIV
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Problems solved by technology

The excellent characteristics of imported Berkshire pigs are mainly high growth performance, but there are also problems and deficiencies such as disease resistance and reduced fecundity. rate, piglets born alive and survival of weaned piglets decreased

Method used

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  • Breeding method of new Berkshire pig disease-resistant variety

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Embodiment Construction

[0045] The present invention will be further described below in conjunction with specific examples.

[0046] (1) Carry out F18 Escherichia coli (purchased by the China Veterinary Drug Administration) infection test to the core group of Berkshire pigs by adding feed, according to the results of phenotypic identification, set up the basic group of Berkshire pig F18 Escherichia coli disease-resistant breeding;

[0047] 1) Bacterial culture

[0048] Inoculate a single colony of F18 Escherichia coli into 3-5 mL of LB medium, and culture it with shaking at 37°C overnight (about 18 hours). The next day, the bacteria were repeatedly centrifuged and washed 3 times with PBS, and the Escherichia coli strain was mixed with PBS according to 1 :20 ratio to dilute, adjust the bacterial concentration to be 4.6×10 8 CFU / L.

[0049] 2) Feed preparation

[0050] Weaned piglets were fed pelleted feed containing 21.7% crude protein according to their nutritional requirements. The feed did not c...

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Abstract

The invention relates to a breeding method of a new Berkshire pig disease-resistant variety. The breeding method comprises the steps that firstly, weaned piglets of a Berkshire pig core group are subjected to Escherichia coli infection experiments by adopting a feed adding method, according to a phenotype identification result, a Berkshire pig Escherichia coli disease-resistant breeding base group is established; secondly, the base group is subjected to molecule breeding with general resistance to diseases by using a proprietary technology obtained in the early stage, meanwhile characters such as breeding performance, growing speed, lean meat percentage and carcass quality are detected and selected, and a first generation is built; thirdly, a group continuous progeny breeding and molecular marker assisted selection method is adopted, after four to five generations, an established breeding aim is achieved step by step, and a new variety core group is established. The new Berkshire pig disease-resistant variety has the advantages of resistance to weaned piglet diarrheal diseases, high stress resistance, high environmental adaptability and the like, and can further provide guidance for disease-resistant breeding and new variety selection and breeding of other pig varieties.

Description

technical field [0001] The invention relates to the technical fields of animal genetics, breeding and reproduction, and molecular biology, in particular to a method for cultivating a new Berkshire pig disease-resistant strain. Background technique [0002] Pig disease is the enemy of pig production, and enterotoxigenic Escherichia coli (ETEC) is the most common and important pathogenic bacteria for diarrhea and edema in newborn and weaned piglets, and is an important threat factor in pig industry (Boldin B, 2008 ), among which F18 Escherichia coli is one of the most common and most harmful Escherichia coli pathogens in the swine industry (Van den BroeckW et al, 2000). Although some preventive measures currently taken against piglet diarrhea (such as improved management, improved sanitation, injection of drugs, and subunit vaccination, etc.) have reduced the incidence of the disease to a certain extent, they have not fundamentally eliminated weaning diarrhea. The occurrence ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/0276
Inventor 包文斌刘颖吴圣龙黄小国是英豪魏宗友
Owner YANGZHOU UNIV
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