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Bacterial strain capable of preventing and treating cattle and sheep diarrheal diseases

A microbial strain, Bacillus subtilis technology, is applied in the field of bacterial strains for preventing and treating diarrheal diseases in cattle and sheep, and can solve the problems of decreased growth and production performance, late start of research on probiotics, and destruction of micro-ecological balance, etc. Immunity, strong antibacterial effect

Active Publication Date: 2019-01-08
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. When the animals lack probiotics, the micro-ecological balance will be destroyed, and the animals will show various diseases and sub-health states, and the growth and production performance will decline
Compared with developed countries, the research on probiotics in my country started late and has a relatively weak foundation. Although some functional products related to probiotics have appeared in the domestic market, most of the strains used are purchased or introduced from abroad. Known Commercial Probiotic Strains

Method used

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  • Bacterial strain capable of preventing and treating cattle and sheep diarrheal diseases
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  • Bacterial strain capable of preventing and treating cattle and sheep diarrheal diseases

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Embodiment Construction

[0022] The scheme of the present invention is described in detail below in conjunction with the accompanying drawings.

[0023] 1. Isolation of strains

[0024] The bacillus subtilis JT3-1 (Bacillus subtilis JT3-1) of the present invention is isolated from healthy yak feces in Gansu. The strain separation and purification method of the present invention is as follows: take 10 g of Gansu yak feces, dilute, mix and filter with 100 mL TS Broth, take 1 mL of the mixed liquid for centrifugation, and discard the supernatant. Add an appropriate amount of nutrient broth, vortex, place in a 37°C incubator and shake for 48h, bathe in 80°C water for 10min, centrifuge, and discard the supernatant. Add an appropriate amount of nutrient broth, vortex, and shake in a 37°C incubator for 24 hours. Appropriate amount of dilution spreads on nutrient agar (NA) plates. Select 6 single colonies of suspected Bacillus subtilis colonies, streak and purify them on the nutrient agar (NA) plate for 3 ...

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Abstract

The invention relates to a bacterial strain capable of preventing and treating cattle and sheep diarrheal diseases. The bacterial strain capable of preventing and treating the cattle and sheep diarrheal diseases is bacillus subtilis and preserved in China General Microbiological Culture Collection Center; and the preservation number is CGMCC NO: 15545. The bacillus subtilis can be applied to plant-eating animal feeding, can also be applied to preparation of plant-eating animal feed additives and can further be applied to preparation of plant-eating animal feed. The bacterial strain provided bythe invention has relatively strong adhesion capacity and antibacterial capacity, has very good acid-resistant capacity, gastrointestinal tract resistant capacity and bile salt tolerance, and can effectively prevent and treat the cattle and sheep diarrheal diseases.

Description

technical field [0001] The invention relates to a bacterial strain capable of preventing and treating diarrheal disease in cattle and sheep. Background technique [0002] Since the 20th century, with the widespread application of antibiotics in feed-grade additives, antibiotics have played an indelible and important role in human medical treatment, livestock and poultry production, disease prevention and control, promotion of animal growth, and increase in the quantity of meat products. In the modern animal husbandry industry, the extensive use of antibiotics in the production of livestock and poultry feed has become the norm. Statistics show that about 50% of the global total antibiotic production is used in the animal husbandry industry. However, due to the long-term use and abuse of antibiotics, their disadvantages are increasingly widely recognized. More and more studies have found that there are many safety problems in the application of antibiotic additives, such as t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/10C12R1/125
CPCA23V2002/00A23K10/18A23K50/10C12N1/205C12R2001/125A23V2200/3204
Inventor 李有全李禤关贵全刘军龙刘爱红王锦明殷宏罗建勋刘志杰独军政高闪电刘光远任巧云陈泽杨吉飞
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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