Bacterial strain capable of preventing and treating cattle and sheep diarrheal diseases
A microbial strain, Bacillus subtilis technology, is applied in the field of bacterial strains for preventing and treating diarrheal diseases in cattle and sheep, and can solve the problems of decreased growth and production performance, late start of research on probiotics, and destruction of micro-ecological balance, etc. Immunity, strong antibacterial effect
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[0022] The scheme of the present invention is described in detail below in conjunction with the accompanying drawings.
[0023] 1. Isolation of strains
[0024] The bacillus subtilis JT3-1 (Bacillus subtilis JT3-1) of the present invention is isolated from healthy yak feces in Gansu. The strain separation and purification method of the present invention is as follows: take 10 g of Gansu yak feces, dilute, mix and filter with 100 mL TS Broth, take 1 mL of the mixed liquid for centrifugation, and discard the supernatant. Add an appropriate amount of nutrient broth, vortex, place in a 37°C incubator and shake for 48h, bathe in 80°C water for 10min, centrifuge, and discard the supernatant. Add an appropriate amount of nutrient broth, vortex, and shake in a 37°C incubator for 24 hours. Appropriate amount of dilution spreads on nutrient agar (NA) plates. Select 6 single colonies of suspected Bacillus subtilis colonies, streak and purify them on the nutrient agar (NA) plate for 3 ...
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