Survivability-screened cancer stem cells, preparation method, application and kit as well as antigen composite thereof, preparation method, application and kit

A technology of tumor stem cells and kits, which is applied in the direction of anti-tumor drugs, drug combinations, and treatment of microorganisms with electricity/wave energy, which can solve the problems of high recurrence and metastasis rates, achieve a radical cure of recurrence and metastasis, and overcome tumor tolerance Effect

Inactive Publication Date: 2009-09-23
北京弘润天源基因生物技术有限公司
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AI Technical Summary

Problems solved by technology

[0007] In order to overcome the existing cancer immunotherapy methods targeting common tumor stem cells still have the problems of tumor resistance after radiotherapy and chemotherapy, especially increased drug resistance, high recurrence rate and high metastasis rate, the purpose of the present invention is to provide a A tolerance-screened tumor stem cell and a preparation method thereof, an application of the tolerance-screened tumor stem cell in the preparation of antitumor drugs, a kit for preparing the tolerance-screened tumor stem cell, and a preparation method of the tolerance-screened tumor stem cell Antigen composition obtained from tumor stem cells screened for tolerance and preparation method thereof, application of antigen composition obtained from tumor stem cells screened for tolerance in the preparation of antitumor drugs, preparation of Antigen composition kit obtained from tumor stem cells

Method used

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  • Survivability-screened cancer stem cells, preparation method, application and kit as well as antigen composite thereof, preparation method, application and kit
  • Survivability-screened cancer stem cells, preparation method, application and kit as well as antigen composite thereof, preparation method, application and kit
  • Survivability-screened cancer stem cells, preparation method, application and kit as well as antigen composite thereof, preparation method, application and kit

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preparation example Construction

[0043] The present invention provides a method for preparing tumor stem cells screened by tolerance, wherein the method includes:

[0044] a step of irradiating and / or screening for chemotherapeutic drugs on tumor stem cells,

[0045] The radiation irradiation screening is accomplished by exposing the tumor stem cells to a radiation source of radiation selected from the group consisting of X-rays, beta-rays, alpha-rays and gamma-rays,

[0046] The chemotherapeutic drug screening is completed by contacting tumor stem cells with chemotherapeutic drugs, and the chemotherapeutic drugs are selected from alkylating agent chemotherapeutic drugs, nitrosourea chemotherapeutic drugs, antimetabolite chemotherapeutic drugs, antibiotic chemotherapeutic drugs, plant chemotherapeutic drugs, hormones and endocrine drugs Chemotherapy drugs, antibody blocker chemotherapy drugs, phytochemotherapy drugs, antibody blocker chemotherapy drugs, asparaginase, procarbazine, dacarbazine, cisplatin, carb...

preparation example 1

[0129] Preparation Example 1: Isolation and Culture of Tumor Stem Cells in Breast Cancer Tissue

[0130] The breast cancer tissue came from a 39-year-old female patient who was diagnosed with breast cancer by pathological examination. An informed consent was signed with the patient, and his tumor tissue was collected during the operation. Cut the breast cancer tissue into about 1mm with ophthalmic scissors 3 of small pieces. The resulting chopped tissue pieces were digested with 0.25% trypsin solution at a weight-to-volume ratio of 1 g: 0.5 mL for 5 minutes, and then calf serum (U.S. Hyclone Company), which was one-fifth of the volume of the trypsin solution, was added to stop. Centrifuge at 1000rpm to collect the digested tissue fragments, pour off the supernatant of the trypsin solution, and then use HBSS containing 0.1% dispase (dispase, American Sigma) (1IU / mg) and 0.01% DNAse (DNase, Shanghai Shenggong) (Hans buffer, Shanghai Sangong) was washed three times with a weig...

preparation example 2-4

[0136] The tumor stem cells of colon cancer, prostate cancer, and brain glioma were respectively prepared according to the method of Preparation Example 1. Among them, the differences are as follows:

[0137] Colon cancer tissue was obtained from a 40-year-old male patient who was diagnosed with moderately differentiated colon adenocarcinoma by pathological examination. An informed consent was signed with the patient, and his tumor tissue was collected during the operation.

[0138] Prostate cancer tissue was obtained from a 49-year-old male patient who was diagnosed with prostate cancer by pathological examination. An informed consent was signed with the patient to collect his tumor tissue at the time of biopsy.

[0139] The glioma tissue was obtained from a 50-year-old male patient who was diagnosed with astroglioma by pathological examination. An informed consent was signed with the patient, and his tumor tissue was collected during the operation.

[0140] Table 2

[0...

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Abstract

The invention provides survivability-screened cancer stem cells, a preparation method thereof, the application of the survivability-screened cancer stem cells in preparing antineoplastic agents, a kit for preparing the survivability-screened cancer stem cells, an antigen composite which is obtained from the survivability-screened cancer stem cells, a preparation method thereof, the application of the antigen composite in preparing antineoplastic agents and a kit for preparing the antigen composite. The antineoplastic agents which are prepared by the survivability-screened cancer stem cells and the antigen composite which is obtained from the survivability-screened cancer stem cells can stimulate inner body immune response and outer body immune response to kill the cancer stem cells which cause tumor recurrence so as to provide a probability for conquering the survivability of the tumor and radically curing the recurrence and the metastasis of the tumor.

Description

technical field [0001] The present invention relates to tumor stem cells (TSC) and a preparation method thereof, the application of the tumor stem cells in the preparation of antitumor drugs, a kit for preparing the tumor stem cells, an antigen composition obtained from the tumor stem cells and a preparation method thereof, and the Application of the antigen composition obtained from tumor stem cells in the preparation of antitumor drugs, and a kit for preparing the antigen composition obtained from the tumor stem cells. [0002] In particular, the present invention relates to tolerance-screened tumor stem cells and a preparation method thereof, the application of the tolerance-screened tumor stem cells in the preparation of antitumor drugs, and a kit for preparing the tolerance-screened tumor stem cells , and the antigen composition obtained from the tumor stem cells screened by tolerance and its preparation method, the application of the antigen composition obtained from the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N13/00C12Q1/02C07K14/435A61P39/00A61P35/00
Inventor 沈丽郝丽敏王亚军马艳玲黄启明
Owner 北京弘润天源基因生物技术有限公司
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