Method for extracting high-purity astragaloside from astragalus mongholicus

Abstract

The invention discloses a method for extracting high-purity astragaloside from astragalus mongholicus, and belongs to the technical field of biological medicines. The method comprises the following steps: (1) taking a ground astragalus mongholicus root, adding water, carrying out reflux extraction for 2-4 times at normal temperature, combining water extraction solutions, concentrating until the density is 1.2-1.3, adding ethanol, stirring for depositing fully, centrifuging, filtering and collecting a supernatant solution; (2) placing the supernatant solution into a reaction kettle, adding 10% of alkali solution by mass concentration for adjusting the pH value to be 10, reacting for 8-12 h at the temperature of 40 DEG C, adding dilute acid for adjusting the pH value to be neutral, extracting by using normal butanol and concentrating; and (3) adding ethyl acetate into a normal butanol concentrated solution for extracting, and re-crystallizing by using methanol to obtain a white crystal, which is a target product-astragaloside. The method is simple and easy to implement, the yield of a product is more than 0.08%, the purity of the product reaches more than 98%, the product has no chemical reagent residues, and the extraction process is environment-friendly and low in requirement on reagent and equipment and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a method for extracting astragaloside IV from astragalus. Background technique [0002] Astragalus is a leguminous plant, which is widely planted in my country as a raw material of traditional Chinese patent medicine. It is used as a tonic in traditional medicine. It has the characteristics of sweet taste and mild temperature. Modern medical research shows that the main component of astragalus is astragaloside IV. Its clinical efficacy can improve cardiopulmonary function, dilate blood vessels, lower blood pressure, protect the liver and prevent the reduction of liver glycogen, and has a good effect on chronic hepatitis. Astragaloside IV also has the function of inducing interferon and mobilizing the body's immune function. It can inhibit virus replication and tumor growth, enhance the body's immunity, increase the body's energy, resist fatigue, and inhibit the effect of osteoc...

AI Technical Summary

Problems solved by technology

[0003] The purity of astragaloside IV currently on the market is not high, most of which are lower than 80%, which affects the medical efficacy of astragaloside IV. In order to obtain astragaloside IV products with higher purity, domestic scientists have done related research work , Patent No. 200310108915.6, the name of the invention is "Preparation method of high-purity astragaloside IV", which discloses an extraction method including extraction, desugaring, decolorization, and purification steps; Method for Separating Astragalus Hepatitis A", using homogenate extraction-mixed enzyme-induced biotransformation technology, negative pressure cavitation and swirling extraction technology, hydrolysis conversion technology of saponin derivatives, liquid-liquid extraction technology, macroporous adsorption resin enrichment technology, normal phase A series of high-efficiency extraction, separation and purification techniques such as silica gel medium-pressure column chromatography technology, low-temperature crystallization and recrystallization technology, the conversion rate obtained is 0.08%, and the purity of astragaloside IV is about 95%.

In the above prior art, in the whole extraction process, the steps are cumbersome, the equipment used is more and expensive, and the required reagents all include expensive macroporous adsorption resins, which are expensive and have low production efficiency.