Application of the effective part of Chinese medicinal material lindley eupatorium herb in preparing antiviral medicine

A drug, wild horse technology, applied in the application field of oral or injectable drugs, can solve the problems of unsustainable immunity and unrealistic prevention.

Inactive Publication Date: 2004-04-07
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the wide variety of viruses, the wide range of infections, and the short-lasting immunity, it must r

Method used

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  • Application of the effective part of Chinese medicinal material lindley eupatorium herb in preparing antiviral medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The results of preliminary screening test of anti-SARS virus drug in vitro by total flavonoids of Yemachai

[0025] (1) Experimental materials

[0026] 1. Cells: VeroE6 cells (African green monkey kidney cells).

[0027]2. Virus: SARS virus, BJ-01 strain.

[0028] 3. Culture medium: DMEM culture medium containing 10% fetal bovine serum.

[0029] 4. Drugs: Yemazhui flavonoids extract, provided by Jiangsu Academy of Traditional Chinese Medicine

[0030] (2) Experimental steps

[0031] 1. The CPE method was used to measure the half toxic concentration (TCID) of the virus to VeroE6 cells 50 ).

[0032] 1. CPE method combined with MTT method to determine the non-toxic concentration of drugs on cells.

[0033] 2. The maximum non-toxic concentration of the drug is used to measure the efficacy of the SARS virus.

[0034] (3) Experimental results

[0035] 1. Virus TCID 50 for 10 -7 .

[0036] 2. Take 4 concentrations of the drug, namely 200, 100, 10 and ...

Embodiment 2

[0039] Embodiment 2: Antiviral test of total flavonoids from Yema chasing

[0040] (1) Source of materials

[0041] Drug: Yemazhu total flavonoid extract, the concentration is 10.64mg / ml, provided by Jiangsu Academy of Traditional Chinese Medicine.

[0042] Virus strain:

[0043] (1) Influenza virus A / FM1 / 47 / H1N1 (belongs to orthomyxovirus, preserved in this room, passed down in chicken embryo);

[0044] (2) Sendai virus (a paramyxovirus, kept in this laboratory).

[0045] Cells: MDCK, a diploid passage strain of human embryonic lung.

[0046] (2) Method

[0047] The virus used was subcultured in advance, and 100TCID was taken after titration 50 is the amount of infection.

[0048] How to deal with drug-virus interactions:

[0049] ① The drug acts on the cells at 100ug / ml, 30ug / ml, 10ug / ml first, and then add 100TCID after 24 hours 50 influenza virus or Sendai virus;

[0050] ② drug 100ug / ml, 30ug / ml, 10ug / ml plus 100TCID 50 Influenza virus or Sendai virus overnight ...

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Abstract

Lindley eupatorium herb total flavone and its pharmaceutically acceptable salt is used in preparing medicine for preventing and treating virus diseases, especially SARS. Lindley eupatorium herb total flavone is selected from quercetin, kaempferol, jaceidin, hyperoside, trifolirhizin, astragaloside or their mixture. Lindley eupatorium herb total flavone in the concentration of 100 microgram/ml has no toxicity to VeroE6 cell and complete supperssion on SARS virus.

Description

technical field [0001] The invention relates to the application of the effective fraction of the traditional Chinese medicine Yemazhui in the preparation of antiviral drugs, especially the application in the preparation of anti-SARS virus oral or injection drugs. Background technique [0002] Respiratory system infectious diseases include upper respiratory tract infection and lung infection, which are inflammations caused by viruses and bacteria, often accompanied by symptoms such as headache, fever, cough, and phlegm, including common colds, flu, tracheitis, bronchitis, Pneumonia, adenovirus disease, syncytial virus disease, etc. At present, Western medicine has no specific medicine for viral infectious diseases, and the treatment is mainly based on supporting symptomatic treatment. Due to the wide variety of viruses, the wide range of infection, and the short-lasting immunity, it must rely on repeated re-infection to maintain it. Therefore, it is unrealistic to widely use...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K9/16A61K9/20A61K9/48A61K31/7048A61K31/78A61P11/00A61P31/12A61P31/14
Inventor 段金廒杨念云钱士辉沈明勤钱大玮彭蕴茹
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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