Method for cultivating adventitious root tissue of wild panax ginseng C.A.Mey.

A wild ginseng and root tissue technology, applied in the field of plant tissue culture, can solve the problems of exogenous gene safety, low ginsenoside content, shorten breeding time, etc., and achieve the effects of fast adventitious root proliferation, high induction rate, and low cost

Inactive Publication Date: 2018-07-13
TIANJIN UNIV
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Problems solved by technology

However, this technique has not been used commercially due to potential safety concerns with the introduction of exogenous genes during hair root induction
[0004] Also have report to carry out the method for tissue culture production ginsenoside with ginseng adventitious root, it has advantages such as safety, stability, easy operation, plant tissue culture technology can obtain a large amount of rege...

Method used

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  • Method for cultivating adventitious root tissue of wild panax ginseng C.A.Mey.
  • Method for cultivating adventitious root tissue of wild panax ginseng C.A.Mey.
  • Method for cultivating adventitious root tissue of wild panax ginseng C.A.Mey.

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preparation example Construction

[0028] 2) Preparation of standard solution

[0029] Accurately weigh 1 mg each of the standard ginsenosides Rg1, Re, Ro, Rf, Rb1, Rg2, Rh1, Rc, Rb2, Rb3, Rd, Rg3, and Rh2 that have been dried under reduced pressure for more than 24 hours, and place them in a 5mL volumetric flask. Ultrasonic dissolve methanol, then dilute to volume with methanol to make 0.2mg·mL -1 The ginsenoside mixed standard stock solution was sealed and stored in a refrigerator at 4°C.

[0030] 3) Preparation of sample solution

[0031] Accurately weigh 0.2g of dried adventitious root sample and place it in a 100mL Erlenmeyer flask with a stopper, add 20mL of methanol, bathe in 60°C water for 1h, suction filter while hot, repeat the extraction once, and combine the filtrates. The filtrate was evaporated to dryness, then dissolved in 10 mL of distilled water, extracted twice with 20 mL of n-butanol, and the n-butanol layer was collected, treated by rotary evaporation, and then dilute to 1 mL with methanol...

Embodiment 1

[0033] The first solid medium is MS basic medium with 2.0mg / L 2,4-dichlorophenoxyacetic acid, 0.5mg / L 6-furfurylaminopurine, 3% sucrose, pH 5.8, at 121 ℃, 0.1MPa pressure sterilization for 25 minutes.

Embodiment 2

[0035] The second solid medium was MS basal medium with 5.0 mg / L indolebutyric acid, 3% sucrose, pH 5.8, sterilized at 121° C. and 0.1 MPa pressure for 25 minutes for use.

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Abstract

The invention discloses a method for cultivating adventitious root tissue of wild panax ginseng C.A.Mey.. The method comprises the following steps of: (1) induction of callus of wild panax ginseng C.A.Mey.; (2) proliferation of the callus of wild panax ginseng C.A.Mey.; (3) induction of adventitious roots of wild panax ginseng C.A.Mey.; and (4) proliferation of the adventitious roots of wild panaxginseng C.A.Mey. in a liquid medium. The method has rapid proliferation of the adventitious roots, high-content saponin compounds, a simple process, low cost, a high induction rate, good repeatability, a short period and no influence of the natural environment at the same time.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for wild ginseng adventitious root tissue culture. Background technique [0002] Wild ginseng, Araliaceae Panax genus, is mainly distributed in China, North Korea, Russia and other regions. Wild ginseng is a ginseng that has grown naturally in deep mountains and dense forests for more than 15 years. Wild ginseng is a nourishing and strong medicine, and it is well known in the world. The chemical components contained in wild ginseng include ginsenosides, sugars, amino acids, vitamins, proteins, polypeptides, organic acids, fat-soluble components and trace elements, etc., which have special nutritional value and good therapeutic effect. Saponin is the most important physiologically active substance of wild ginseng. It is recorded in "Shen Nong's Herbal Classic": "Ginseng can nourish the five internal organs, calm the spirit, calm the soul, stop panic, elimina...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 高文远王娟梁文霞姚陆王诗惠
Owner TIANJIN UNIV
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