Quick propagation method for lycoris chinensis

A Lycoris, fast technology, applied in the field of rapid propagation of Lycoris, to achieve the effect of large-scale production and development, high value, good consistency, and good economic prospects

Inactive Publication Date: 2011-10-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is very little research on Lycoris chinensis, and there is no m

Method used

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  • Quick propagation method for lycoris chinensis
  • Quick propagation method for lycoris chinensis
  • Quick propagation method for lycoris chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The MS in this example is conventional MS containing 30 g / L sucrose and 6 g / L agar.

[0029] Choose healthy and plump Chinese Lycoris seeds, such as figure 1 As indicated, wash the surface of the seeds with detergent solution, rinse with tap water for 0.5-1 h, and rinse with distilled water for 3 times. Treat the seeds with 70% alcohol for 30s, rinse with sterile water 3 times, about 30s each time; then use 0.11%HgCl 2 Soak and sterilize for 12~15min, rinse with sterile water 4~6 times, 30s each time. Cut the seeds with a scalpel, and take out the embryos in the seeds with tweezers, figure 2 As shown, it was inoculated on the sterilized callus induction medium MS+2,4-D2.0~3.0mg / L to induce callus. The callus induction process was carried out under dark conditions, and the callus began to emerge after about 10 days of culture, and gradually proliferated and grew up, such as image 3 Shown, about the 25th day, the growth state of callus is the best. At this time, th...

Embodiment 2

[0031] Carry out embodiment 1 repeatedly according to described same step, in the induction stage of callus, the culture medium of induced callus is replaced by MS+6-BA0.1~0.5mg / L+2,4-D1.0~2.0 mg / L+CH250~500mg / L. Adding 250~500 mg / L of hydrolyzed casein to the medium not only increases the callus rate of the embryo explants, but also greatly improves the quality of the induced callus.

Embodiment 3

[0033] The method is the same as in Example 1. In the bulblet culture stage, when the sucrose concentration in the medium for inducing bulblets is increased from 30g / L to 40-60g / L, the diameter of the bulblets can be increased from about 0.4cm to 0.7cm. About cm, it can obviously promote the expansion of bulbs and play a role in strengthening seedlings.

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Abstract

The invention discloses a quick propagation method for lycoris chinensis. The method comprises the following steps of: taking embryos of the lycoris chinensis, and inoculating the embryos to an induction medium to induce callus; transferring the callus to an MS (Murashige and Skoog) culture medium containing sucrose, naphthyl acetic acid (NAA) and 6-benzyl aminopurine (6-BA) to induce adventitious buds; transferring the adventitious buds to the MS culture medium containing the sucrose, the NAA and the 6-BA to induce bulblets; transferring the expanded bulblets to an MS culture medium containing agar and NAA, and performing induced rooting under illumination; and transferring the rooted bulbs to a transplanting medium of perlite and humus. According to the method disclosed by the invention, the embryos of the lycoris chinensis are used as explants, the explants are induced to generate the callus, then the adventitious buds and the bulblets are induced, and plant regeneration is finally completed, so that a new path is increased for quick propagation of tissue culture of the lycoris chinensis. The method has high propagation coefficient, millions of bulblets can be produced in one year, and an effective path can be provided for quickly propagating seed bulbs; and the test tube plantlets have high differentiation synchronizing degree and good consistency, and the method has high large-scale production development value and good economic prospect.

Description

technical field [0001] The invention relates to a rapid propagation method of lycoris, in particular to a method for rapid propagation of Chinese lycoris by tissue culture. Background technique [0002] Lycoris is a bulbous plant, and there are about 20 species in the world, and 15 species in my country, accounting for three-quarters of the global total. The plants of the genus have bright colors and unique flower shapes, and are promising fresh-cut flower materials; because of their leaves and growth in winter and spring, they are rare winter and spring ground cover plants in the middle and lower reaches of the Yangtze River; Bulbs contain high content of valuable biopharmaceutical raw materials such as galantamine and lycorine, which are good medicines for treating senile dementia, so plants of the genus Lycoris have extremely high economic value. [0003] Chinese Lycoris chinensis is a species of Lycoris chinensis, native to my country, is an economical plant that integr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 周坚戴萍张春霞
Owner NANJING FORESTRY UNIV
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