Gerbera jamesonii receptacle induction method

Abstract

The invention provides a gerbera jamesonii receptacle induction method and relates to a flower culture method. The gerbera jamesonii receptacle induction method comprises the following steps of: selecting explants, disinfecting the explants, selecting receptacle induction media and selecting culture conditions and modes. Tender gerbera jamesonii buds are vertically cut into four blocks after calyx and small inflorescence peeling and are then inoculated into the induction culture medium for normal light illumination culture. The method is characterized in that in the step of selecting the explants, explants with the bud diameter being 0.5 to 0.8cm are used as selection objects, and in addition, the cold storage pre-treatment on the selected objects for 48 hours at 4 DEG C is added; in the step of selecting receptacle induction media, the selected culture medium has a recipe consisting of 10mg / L 1 / 2MS+6-BA, 0.5mg / L of NAA, 30g / L of cane sugar and 7g / L of agar, and the pH value is controlled to be about 5.8; and the culture conditions and modes are as follows: 7-day dark pre-culture is firstly carried out, then, the normal light illumination culture is adopted, the light illumination intensity is 1500 to 2000lx, the light illumination time is 14hours / day, and the temperature is 23 to 25 DEG C until annual seedlings are obtained through culture. The induction rate of the receptacle callus and the differentiation rate of adventitious buds are improved.

Description

Technical field: [0001] The invention relates to a method for cultivating flowers, and is particularly suitable for the induction method of gerbera receptacles. Background technique: [0002] Gerbera, scientific name Gerbera jamesonii Bolus, is a perennial herbaceous plant of the genus Gerbera in the Compositae family. It is one of the five largest cut flowers in the world. Therefore, it is favored by consumers. Gerbera can be propagated by ramets, but at present, most of the gerbera seedlings are produced by tissue culture for rapid propagation, because young flower buds have strong redifferentiation ability, and are often used as explants for gerbera seedling induction. In the prior art, the young flower buds of gerbera are generally cut into four pieces after peeling off the calyx and small inflorescence, inoculated in the induction medium, and cultivated under normal light. This method has a low induction rate, and the induction rate among different varieties exists. T...

AI Technical Summary

Problems solved by technology

[0003] Studies have shown that the developmental stage of the receptacle directly affects the differentiation of the callus, and the receptacle induction effect of the flower head with a diameter of less than 0.7cm in the early stage of bud development is the best. With the increase of the diameter of the inflorescence, the differentiation induction rate decreases, and the infection rate increased