Method for in vitro inducing winter date anther callus

A technology for callus and callus induction, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of low callus induction rate, speed up haploid breeding process, etc., and achieve improved induction rate effect
CN1934932AInactive Publication Date: 2007-03-28HEBEI AGRICULTURAL UNIV.
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEBEI AGRICULTURAL UNIV.
Publication Date
2007-03-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a method capable of adopting isolated induction process to obtain winter jujube anther callus. It is characterized by that said invention utilizes tissue culture of winter jujube anther to obtain its callus. Said method includes the following several steps: explant collection, disinfection, in initial induction and enrichment culture. Besides, said invention also provides the concrete requirements of the above-mentioned every step and its concrete operation method.
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Description

technical field

[0001] The invention relates to a method for in vitro inducing anther callus of winter jujube. Background technique

[0002] Haploid breeding mainly refers to obtaining haploid plants through anther culture or free microspore culture (hereinafter referred to as microspore culture), and then doubling into double haploid plants by colchicine and other treatments, so as to directly obtain homozygous strains. Whether it is anther culture or microspore culture, it generally only takes about 1 year from the beginning of culture to obtaining a completely homozygous strain. For fruit trees, haploid breeding may enable some fruit trees that were originally asexually propagated to be propagated by seeds, and provide pure line materials for the genetic research of fruit trees with highly heterozygous genotypes, thereby promoting the research on the theoretical work of fruit tree genetics . Therefore, if this technology is applied to breeding practice, it will greatly...

Claims

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