Method for in vitro inducing winter date anther callus

A technology for callus and callus induction, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of low callus induction rate, speed up haploid breeding process, etc., and achieve improved induction rate effect

Inactive Publication Date: 2007-03-28
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

Jujube (Ziziphus jujuba Mill.) is a characteristic and dominant fruit tree in my country. So far, the anther culture of jujube has only been reported on Jinsixiaozao, but there is a problem that the callus induction rate is low (43.1%). There is no relevant report on the induction of anther callus of winter jujube, so increasing the induction rate of anther callus of jujube will greatly speed up the haploid breeding process of jujube

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  • Method for in vitro inducing winter date anther callus

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Embodiment Construction

[0014] From May to June, immature flower buds of winter jujube with a field diameter of 0.3-0.6 cm and calyxes not yet opened were selected, and pretreated in a 4°C refrigerator for 1-3 days. Rinse with running water for 1-2 hours, put the flower buds in 70% ethanol for 30s on the ultra-clean workbench, and then transfer to 0.1% HgCl 2 In the solution for 8 minutes, rinse with sterile water for 3 to 4 times, and then peel off the anthers. When starting the culture, inoculate sterile anthers in MS+maltose 15-20g / L+TDZ 0.5-1.0mg / L +NAA 0-0.5mg / L Dark culture was carried out on the culture medium for three weeks, and then transferred to low light (800-1000 Lux) for culture. Anthers gradually produced yellow-green, loose granular callus, and gradually increased with the extension of culture time. Transfer the initial callus obtained during proliferation culture to MS+maltose 15-20g / L+TDZ 0.1-0.5mg / L +NAA 0-0.5mg / L Proliferation culture was carried out in the culture medium, a...

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Abstract

The present invention relates to a method capable of adopting isolated induction process to obtain winter jujube anther callus. It is characterized by that said invention utilizes tissue culture of winter jujube anther to obtain its callus. Said method includes the following several steps: explant collection, disinfection, in initial induction and enrichment culture. Besides, said invention also provides the concrete requirements of the above-mentioned every step and its concrete operation method.

Description

technical field [0001] The invention relates to a method for in vitro inducing anther callus of winter jujube. Background technique [0002] Haploid breeding mainly refers to obtaining haploid plants through anther culture or free microspore culture (hereinafter referred to as microspore culture), and then doubling into double haploid plants by colchicine and other treatments, so as to directly obtain homozygous strains. Whether it is anther culture or microspore culture, it generally only takes about 1 year from the beginning of culture to obtaining a completely homozygous strain. For fruit trees, haploid breeding may enable some fruit trees that were originally asexually propagated to be propagated by seeds, and provide pure line materials for the genetic research of fruit trees with highly heterozygous genotypes, thereby promoting the research on the theoretical work of fruit tree genetics . Therefore, if this technology is applied to breeding practice, it will greatly...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 刘孟军王娜赵锦秦子禹
Owner HEBEI AGRICULTURAL UNIV.
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