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Preparation method of paraffin section of pear flower tissue

A paraffin sectioning and tissue technology, applied in the field of biological histology, can solve the problems of complex operation, unsatisfactory effect, and less research on paraffin sectioning technology, and achieve high quality and improve embedding quality

Inactive Publication Date: 2018-12-21
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are few researches on paraffin section technology carried out on pear blossoms, and the effect is not ideal, and the operation is complicated

Method used

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  • Preparation method of paraffin section of pear flower tissue
  • Preparation method of paraffin section of pear flower tissue
  • Preparation method of paraffin section of pear flower tissue

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Embodiment

[0045] The present embodiment provides a kind of preparation method of the paraffin section of pear flower tissue, comprises the steps:

[0046]S1. Take out the pear flower preserved in the refrigerator at 4-5 degrees Celsius, rinse it with distilled water, remove all stamens, stamens, calyx, petals and 3mm below the base of the flower stalk, and obtain the treated pear flower tissue;

[0047] S2. Put the pear flower tissue obtained in the step S1 into the FAA fixative in the fixation container, and pump air into the fixation container until no bubbles appear in the FAA fixation solution. After the pumping is over, fix it for 1-2 days;

[0048] S3. Place the pear flower tissue obtained after air extraction on gauze to absorb the FAA fixative, and then perform dehydration, transparency, wax dipping, embedding, sectioning, sticking, dewaxing, dyeing and de-floating treatment in sequence.

[0049] In this embodiment, the step S3 is specifically;

[0050] S301 Dehydration: Dehydr...

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Abstract

The invention relates to the technical field of biological histology, in particular to a preparation method of paraffin section of pear flower tissue, which comprises the following steps: taking out the pear flowers preserved in a 4-5 degree Celsius refrigerator and washing them with distilled water, removing the center of the flower, stamen, calyx, petals, and the 3 mm portion below the base of the stalk to obtain the treated pear flower tissue; putting the obtained pear flower tissue into a FAA fixing solution of a fixing container, and evacuating the fixing container until the FAA fixing solution no longer appears bubbles, fixing 1-2days after evacuating; placing the pear flower tissue obtained after evacuating on a gauze to absorb the FAA fixing solution, and carrying out dehydration,transparency, waxing, embedding, slicing, sticking, dewaxing, dyeing and de-floating treatment in turn. According to the preparation method of paraffin section of pear flower tissue, the problem thatthe traditional paraffin section has no strict requirements on material processing, which causes the tissue to be too large when slicing, thereby having a great influence on the experimental result issolved, and the quality of the paraffin section obtained is high.

Description

technical field [0001] The invention relates to the technical field of biological histology, in particular to a method for preparing paraffin sections of plant tissues, in particular to a method for preparing paraffin sections of pear flower tissues. Background technique [0002] Paraffin tissue section technology is the most widely used method in routine histological preparation techniques. It is not only used to observe the morphological structure of normal cell tissue, but also the main method used in pathology and forensic science to study, observe and judge the morphological changes of cell tissue, and has been widely used in research in many disciplines. However, due to the specific tissue structure of plants, the specific operation methods of different materials are also different. [0003] At present, there are few studies on the paraffin section technique carried out on pear blossoms, and the effect is not ideal, and the operation is complicated. Therefore, how to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/2813
Inventor 包建平贡万辉蔡利华马司光董玉忠张朋朋袁志豪
Owner TARIM UNIV
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