Method for acquiring virus-free strawberry seedlings
A technology for detoxifying seedlings and strawberries, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions to achieve the effect of improving the survival rate
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Embodiment 1
[0046] 1) Obtaining and disinfection of explants
[0047] Take strawberry flower buds and rinse them under running tap water for 1-2 hours. Disinfect the surface with 70% alcohol on the ultra-clean workbench for 30 seconds, then disinfect with about 14% hydrogen peroxide for 15 minutes, and then rinse with sterile water for 3 times, then remove the outer bracts of the flower buds and inoculate them in the pre-prepared on the induction medium.
[0048] 2) Selection of hormones
[0049] After routine disinfection of flower buds, the outer sepals of the flower buds were removed on a clean bench, and the treated explants were inoculated on a medium with a concentration of NAA of 0.1 mg / L and a concentration of 6-BA of 1.0 mg / L.
[0050] 3) Induce bud differentiation and propagation
[0051] After the flower buds were cultured on the induction medium for several generations, the callus continued to expand, and the young buds differentiated, and the buds gradually grew into small...
Embodiment 2
[0057] Other steps in this example are the same as in Example 1, the only difference is that in step 2) selection of hormones, the concentration of NAA is 0.1 mg / L, and the concentration of 6-BA is 1.2 mg / L on the culture medium.
Embodiment 3
[0059] Other steps in this example are the same as in Example 1, the only difference is that in step 2) selection of hormones, the concentration of NAA is 0.1 mg / L, and the concentration of 6-BA is 1.5 mg / L on the culture medium.
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