Method for acquiring virus-free strawberry seedlings

A technology for detoxifying seedlings and strawberries, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions to achieve the effect of improving the survival rate

Inactive Publication Date: 2015-03-25
SHAOXING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems existing at present, and provides a new set of methods for rapid propagation of virus-free strawberry seedlings, so as to achieve the purpose of high-efficiency factory production and form a set of rapid propagation techniques for virus-free strawberry seedlings.

Method used

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  • Method for acquiring virus-free strawberry seedlings
  • Method for acquiring virus-free strawberry seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Obtaining and disinfection of explants

[0047] Take strawberry flower buds and rinse them under running tap water for 1-2 hours. Disinfect the surface with 70% alcohol on the ultra-clean workbench for 30 seconds, then disinfect with about 14% hydrogen peroxide for 15 minutes, and then rinse with sterile water for 3 times, then remove the outer bracts of the flower buds and inoculate them in the pre-prepared on the induction medium.

[0048] 2) Selection of hormones

[0049] After routine disinfection of flower buds, the outer sepals of the flower buds were removed on a clean bench, and the treated explants were inoculated on a medium with a concentration of NAA of 0.1 mg / L and a concentration of 6-BA of 1.0 mg / L.

[0050] 3) Induce bud differentiation and propagation

[0051] After the flower buds were cultured on the induction medium for several generations, the callus continued to expand, and the young buds differentiated, and the buds gradually grew into small...

Embodiment 2

[0057] Other steps in this example are the same as in Example 1, the only difference is that in step 2) selection of hormones, the concentration of NAA is 0.1 mg / L, and the concentration of 6-BA is 1.2 mg / L on the culture medium.

Embodiment 3

[0059] Other steps in this example are the same as in Example 1, the only difference is that in step 2) selection of hormones, the concentration of NAA is 0.1 mg / L, and the concentration of 6-BA is 1.5 mg / L on the culture medium.

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Abstract

The invention provides a rapid propagation method for acquiring virus-free strawberry seedlings. The method comprises the following steps: (1) selecting a raw material and sterilizing the raw material; (2) bud culture: removing sepals of a sterilized bud, then inoculating the bud into a prepared culture medium of MS+6-BA1.0mg / L+NAA0.1mg / l for culture till clumpy buds grow out; (3) propagation culture: transferring the clumpy buds onto a culture medium of MS+6-BA0.1mg / L for propagation culture; (4) induced rooting: cutting off strawberry seedlings from the clumpy buds, transferring the strawberry seedlings to the culture medium of 1 / 2MS+NAA0.1mg / L culture medium, inducing the rooting, and transferring the rest of callus to the culture medium of MS+6-BA0.1mg / L for culture; (5) seedling hardening: transplanting the rooted strawberry seedlings into a culture plate from a culture bottle, and carrying out the seedling hardening growth in a greenhouse. By utilizing the method, the method for acquiring the virus-free seedlings is simple, the acquired seedlings are strong, stolon reproduce quickly, and the quality is good.

Description

technical field [0001] This patent relates to a method for industrial production of virus-free strawberry seedlings by utilizing strawberry flower buds, involves methods such as strawberry tissue culture and industrial production of virus-free seedlings, and belongs to the technical field of modern agricultural seedling cultivation. Background technique [0002] In vitro rapid propagation ( in vitro Propagation) and plant virus-free are currently the most widely used and most effective aspects of plant tissue culture. In production, many asexually propagated crops are infected by viruses, resulting in serious degradation of varieties, reduced yield and lower quality. The use of plant meristems for cultivation can remove the virus from newly grown plants and propagate the virus-free seedlings. Virus-free crops produced by this method include potatoes, sweet potatoes, garlic, strawberries, apples, bananas, etc., and have been used in large-scale production. [0003] Strawb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 汤访评张建行
Owner SHAOXING UNIVERSITY
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