Method for obtaining dihaploid plants of sweet peppers

Abstract

The invention provides a method for obtaining doubled haploid (DH) plants of sweet peppers, which comprises the following steps of: (1) selecting buds of a donor plant, the microspores of which are in a development state between nonokaryotic stage with the nucleus located aside and early dikaryotic stage, and pretreating at 4 DEG C for 1-3 days; (2) stripping the calyces off, soaking in alcohol, disinfecting through oscillation with sodium hypochlorite, and cleaning with sterile water; (3) stripping the anthers, and inoculating the anthers on an N4-3 solid-liquid double-layer culture medium by the density that 12-18 anthers are inoculated to a culture dish with diameter of 60 mm; (4) firstly, culturing the anthers for 1-10 days in the dark at 28-35 DEG C, and then, transferring to the condition of 25-28 DEG C to continue culturing in the dark; and (5) culturing for 4-7 weeks, when a large quantity of embryoids occur, transferring leaf type embryoids to an MS basic culture medium free of hormones, and cultivating the seedlings. According to the cultivating method provided by the invention, the occurrence rate of the embryoids breakthrough the limitations of genotype, the culturing efficiency of sweet peppers is greatly increased, and meanwhile, the ratio of the DH plants is also increased.

Description

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AI Technical Summary

Benefits of technology

This patented technology improves the growth rate and purity of sugarcane crops by reducing genetic variations that affect their taste quality or nutritional value. It also enhances the ability for these crop varieties to produce better yields from smaller seed pods compared to traditional methods like pollinators such as corn sorghum (Sorghumbus) fertilizers. Additionally, this process allows for more efficient use of space during harvesting while maintaining good agronomics characteristics.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the efficient production of high-quality consistent red peptide products due to the use of free flowing cultures during reproduction processes. These techniques involve growing sugarcane hybrid seeds containing two types of polypetides called glycerol-3 dipteran interferon-1(GIF), while another technique involves crossing off specific parts of the parental lineage called gametes. Additionally, these methods require further study because current methods result in reduced yields compared to traditional ones.

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