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Strawberry tissue culture fast propagation method

A technology for tissue culture and rapid propagation of strawberries, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, large site requirements, long breeding cycle, etc., and achieve inhibition of growth and organ building. , improve efficiency, the effect of short training time

Inactive Publication Date: 2016-06-08
成都贝瑞生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, strawberries mainly reproduce through vegetative reproduction, but the survival rate is not very high, the reproduction coefficient is low, the reproduction cycle is long, the site requirements are large, and the labor cost is high, which is far from meeting the needs of a large number of consumers.

Method used

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  • Strawberry tissue culture fast propagation method
  • Strawberry tissue culture fast propagation method
  • Strawberry tissue culture fast propagation method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A kind of tissue culture rapid propagation method of strawberry described in the present embodiment, comprises the following steps:

[0028] (1) Explant selection and sterilization: cut strawberry young leaves or sepals, the cut length is 0.5-0.9cm, after cleaning, treat with volume fraction 2% pasteurized solution for 4-6 minutes, rinse clean, Treat with 75% alcohol on the ultra-clean bench for 15-25s, rinse with sterile water for 3-4 times, then treat with 0.1% mercuric chloride for 4-6min, rinse with sterile water for 2-4 times, and then use volume fraction 4 % sodium hypochlorite solution for 3-5 minutes, rinsed with sterile water 2-5 times, and then dried;

[0029] (2) callus induction culture: the explant obtained in step (1) is inoculated in the callus induction medium and cultivated for 2-3 weeks to obtain callus, and the composition of the callus induction medium is: MS basal medium, supplemented with 1.5mg / LTDZ, 1.0mg / LNAA, wherein in the callus induction cul...

Embodiment 2

[0034] The tissue culture rapid propagation method of a kind of strawberry described in the present embodiment, the difference with embodiment 1 is:

[0035] In step (2), the composition of the callus induction medium is: MS basic medium supplemented with 0.5mg / LTDZ and 0.25mg / LNAA;

[0036] In step (3), the composition of the differentiation medium and the proliferation medium are: MS basic medium supplemented with 0.5mg / LTDZ and 0.05mg / LNAA;

[0037] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium supplemented with 0.5 mg / LIAA.

[0038] In this embodiment, in the callus induction culture, the callus generation time is 18 days; in the differentiation and proliferation culture, the time to start differentiation of shoots is 40 days, and the time to start proliferation of shoots is 5 days, and the proliferation coefficient is 15; in the rooting culture, the time to start rooting is 10 days.

Embodiment 3

[0040] The tissue culture rapid propagation method of a kind of strawberry described in the present embodiment, the difference with embodiment 1 is:

[0041] In step (2), the composition of the callus induction medium is: MS basic medium supplemented with 3.0mg / LTDZ and 1.5mg / LNAA;

[0042]In step (3), the composition of the differentiation medium and the proliferation medium are: MS basic medium supplemented with 3.5mg / LTDZ and 0.25mg / LNAA;

[0043] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 0.1 mg / LIAA.

[0044] In this embodiment, in the callus induction culture, the callus generation time is 15 days; in the differentiation and proliferation culture, the time to start differentiation of shoots is 30 days, and the time to start proliferation of shoots is 5 days, and the proliferation coefficient is 13; in the rooting culture, the time to start rooting is 10 days.

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Abstract

The invention discloses a strawberry tissue culture fast propagation method. The strawberry tissue culture fast propagation method comprises the following steps of (1) selection and sterilization of an explant: cutting a strawberry leaf or sepal, cleaning, and sterilizing, so as to obtain the explant; (2) induced culture of a callus: inoculating the explant obtained in step (1) into an induced culture medium of the callus to culture for 2-3 weeks, so as to obtain the callus; (3) differentiation and proliferation culture: inoculating the callus obtained in step (2) into a differentiation culture medium to culture for 5-6 weeks, so as to obtain a differentiation bud; inoculating the differentiation bud onto a proliferation culture medium to culture for 8-9 weeks, so as to obtain a proliferation seedling; (4) rooting culture: separating the proliferation seedling obtained in step (3) into single strains, and inoculating into a rooting culture medium to culture for 2-4 weeks, so as to obtain a strawberry sterile plant. The strawberry tissue culture fast propagation method has the advantages that the tissue culture process is simple and convenient, the culture time is short, the proliferation efficiency is high, the seedlings are regular, the subsequent uniform transplanting and planting management is convenient, the operation is easy, and the industrialized production is realized.

Description

technical field [0001] The invention relates to the technical field of biological tissue culture seedlings, in particular to a tissue culture rapid propagation method for strawberries. Background technique [0002] Strawberry is a perennial herb belonging to the family Rosaceae, also known as cranberry, raspberry, ground berry, etc. It is heart-shaped in appearance, delicious and tender, with juicy flesh and special strong fruit aroma. It is rich in amino acids, fructose, and sucrose , glucose, citric acid, malic acid, pectin, carotene, vitamin B1, B2, niacin and minerals calcium, magnesium, phosphorus, potassium, iron, etc. , Children are of great benefit. Foreign scholars have found that the active ingredients in strawberries can inhibit the growth of cancer. Every 100 grams of strawberry contains 50-100 mg of vitamin C, which is more than 10 times higher than that of apples and grapes. Eating some strawberries after a meal can decompose food fat and facilitate digestion...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗琳李飞王洁丁龙梅栗丹罗丽君
Owner 成都贝瑞生态农业开发有限公司
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