Soybean sexual hybridization method

A method of hybridization, a unique technology, applied in the field of hybrid breeding, can solve the problems of low hybridization survival rate and low hybridization efficiency, and achieve the effects of improving hybridization efficiency, preventing false hybrid pods, and shortening the marking time

Inactive Publication Date: 2014-10-08
CANGZHOU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And according to the crossbreeding that prior art carries out, hybridization survival rate is generally lower, only 20%-40%, and hybridization efficiency is always at a lower level, only 50-60 flowers / (person·hour)

Method used

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  • Soybean sexual hybridization method
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Examples

Experimental program
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Effect test

Embodiment 1

[0036] (1) Select 40-50 large flower buds full in the middle of the main stem as the female parent;

[0037] (2) The whole inflorescence only retains the flower buds to be hybridized, and the remaining flower buds are completely removed;

[0038] (3) The calyx lobes of the upper part of the calyx are removed, and the cylindrical structure of the sepal base is retained;

[0039] (4) Perform one-time detasseling of 40-50 flower buds as the female parent at 4-7 pm. The operation method is: clamp the upper 1 / 3 of the petals obliquely downward along the direction of the flag petals to the keel petals, slightly Pull up obliquely to the direction of the flag petal, and avoid damaging the stigma during the operation;

[0040] (5) Coil the short thread into a ring-shaped loose buckle, gently wrap it over the flower, and tie it to the flower stalk, avoiding the stigma during the operation;

[0041] (6) before 6 o'clock in the morning on the second day of emasculation, on a plant with ...

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Abstract

The invention relates to the field of hybridization breeding, and specifically relates to a soybean sexual hybridization method. The soybean sexual hybridization method comprises the following nine links: (1) fixing flower buds; (2) removing sprout for the first time, namely only reserving quasi-hybrid flower buds in the whole inflorescence, and completely removing the rest of flower buds; (3) removing calyx, namely removing calyx lobus at the upper half part of the calyx while reserving the basic cylindrical structure of the calyx; (4) castrating; (5) belaying, namely coiling a short wire into an annular slipknot to gently tie a flower stalk after passing through the flower; (6) taking flowers, namely before 6 a.m. the next day after castrating, picking flowers with unopened petals and uncracked carina on a plant with typical male parent variety characters as a pollen taking object; (7) pollinating at 6-8 a.m. the next day after castrating; (8) putting up brass plates; and (9) managing in the later period. The hybrid survival rate and hybrid efficiency are greatly improved according to the soybean sexual hybridization method provided by the invention.

Description

technical field [0001] The invention relates to the field of hybrid breeding, in particular to a soybean sexual hybridization method. Background technique [0002] Hybrid breeding is currently recognized as the most common and effective breeding method. Sexual hybridization technology is widely used as a technical link in hybrid breeding, but the results are different. The survival rate of hybridization is not only related to some environmental conditions and parental materials, but also directly related to the multi-link operations in the hybridization process. And according to the crossbreeding that prior art carries out, hybridization survival rate is generally low, only 20%-40%, and hybridization efficiency is always at a low level, only 50-60 / (person·hour). Contents of the invention [0003] The purpose of the present invention is to greatly improve the hybridization survival rate and hybridization efficiency by effectively controlling the nine links in the hybridiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 曹金锋胡铁欢卢思慧孙永媛高广居吴凤训刘永震刘同才刘宝卿
Owner CANGZHOU ACAD OF AGRI & FORESTRY SCI
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