Method for rapidly propagating sugarcane tissue culture seedlings with spermine

A technology for tissue culture seedlings and sugar cane, which is applied in the field of tissue culture of plant biotechnology, can solve problems such as failure to retrieve spermine, and achieve the effect of simplifying production procedures

Active Publication Date: 2015-03-11
GUANGXI UNIV
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Problems solved by technology

However, research data on the use of spermine for rapid propagat

Method used

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  • Method for rapidly propagating sugarcane tissue culture seedlings with spermine
  • Method for rapidly propagating sugarcane tissue culture seedlings with spermine
  • Method for rapidly propagating sugarcane tissue culture seedlings with spermine

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Embodiment Construction

[0015] The technical solutions of the present invention will be further described below through examples of implementation.

[0016] The method for inducing sugarcane tissue culture shoots with spermine of the present invention comprises the following steps:

[0017] 1. Acquisition of callus: Obtain sugarcane young leaf slices as explants according to conventional methods, and inoculate them with Spm.1.5mg L after routine disinfection. -1 -3.0mg·L -1 In the modified MS solid medium, each batch of experiments was not less than 10 bottles, and each bottle had 5 slices. Cultivate for 10d-25d under conventional culture conditions. Take 3 bottles at random to count the recovery rate, quickly weigh the fresh weight, and record the average value of the 3 bottles (see Table 1).

[0018] Table 1. Different concentrations of Spm. Induction of sugarcane young leaves to form callus implementation cases

[0019]

[0020] in FIG. 1:

[0021] A. Healing rate (%) = the number of cured...

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Abstract

The invention relates to a method for rapidly propagating sugarcane tissue culture seedlings with spermine. The method comprises the following steps: using sugarcane immature leaf slices as explants, vaccinating the explants to an improved MS solid medium of MS+Spm.1.5 mg . L<-1>-3.0mg . L<-1>, regulating the pH to be 5.8-6.0, and performing continuous culture for 10 d-25 d to induce and obtain sugarcane callus tissue agglomerates; retransferring the agglomerates to the same fresh culture medium for subsequent culturing for 15 d, and then separating and cutting obtained callus tissues into small blocks to be revaccinated to the same fresh culture medium; performing subsequent culture for 20d -30d, transferring regeneration seedlings to the same fresh culture medium to be cultured for 20d so as to obtain test-tube seedlings of which the stem length is 5-6cm and the root seedlings are complete. According to the invention, only one culture medium is used from the starting of explant culture to the generation of the complete regenerated plantlets, so that the production process for the sugarcane tissue culture seedlings is obviously simplified, besides, the toxic action of 2,4-D induction callus tissues is removed, and the cost of the used spermine is low, so that the method is more favorable for popularization.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of plant biotechnology, in particular to a method for rapidly propagating sugarcane tissue culture seedlings with spermine. technical background [0002] "Tissue culture seedlings", also known as "test-tube seedlings", "sterile seedlings" and "clone (Clone) seedlings", are based on the totipotency theory of cells, using explants, in sterile and suitable medium and light temperature Under the condition of rapid breeding, the complete regenerated plants with complete roots and seedlings can be obtained. In the same period of time, the propagation speed of tissue culture seedlings can be dozens or even hundreds of times higher than the natural reproduction speed of plants, and it is disease-free and non-toxic (Virus), and the group is neat, which is most suitable for large-scale industrialization of improved crop varieties. to plant. Tissue culture seedling production technology is one of th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 许鸿源周凤珏蓝桃菊龚银花梁琼月许皓翔
Owner GUANGXI UNIV
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