Hypsizygus marmoreus strain and breeding method thereof

A technology of shimeji mushrooms and strains is applied in the field of edible fungus breeding, which can solve the problems of single strains and the like, and achieve the effect of improving economic benefits.

Active Publication Date: 2019-08-30
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the single problem of the common species of Shiji mushroom used in the existing factory cultivation, a...

Method used

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  • Hypsizygus marmoreus strain and breeding method thereof
  • Hypsizygus marmoreus strain and breeding method thereof
  • Hypsizygus marmoreus strain and breeding method thereof

Examples

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Embodiment 1

[0040] A new variety of edible fungus jijiji mushroom is obtained by crossing the following methods:

[0041] (1) Remove the caps of the uncapped seafood mushroom strains and crab-flavored mushrooms respectively, and place them in a sterile empty petri dish, so that the caps can autonomously eject the spores to the surface of the empty petri dish, and then collect them with 1 mL of distilled water. 2 mL PE tube;

[0042] (2) Under sterile conditions, dilute the spores collected in step (1) until 2-3 spores are observed under a 40-fold microscope, and then spread and culture on PDA medium. When awn-like single colonies appear At the same time, pick the tip hyphae of a single colony and culture it in a flat PDA medium, and cultivate it at a constant temperature of 25°C for 150h;

[0043] (3) Under sterile conditions, draw 7uL of lactic acid carbolic acid cotton blue staining solution on the glass slide, pick the hyphae cultured in step (2) and place them in the staining solutio...

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Abstract

The invention provides a hypsizygus marmoreus strain and a breeding method thereof. A hybrid strain is obtained by the polyspore hybridization of seafood mushroom and crab mushroom, and the hypsizygusmarmoreus variety Hypsizygus marmoreus No.1 is obtained by the polyspore selfing of the hybrid strain. According to the invention, the Hypsizygus marmoreus No.1 is obtained through hybridization andselfing breeding. The growth speed of the Hypsizygus marmoreus No.1 is faster than that of the parent strain, the bag filling time is 5 days earlier than that of the original strain of the seafood mushroom, the growth period is 10 days earlier than that of the parent seafood mushroom, the Hypsizygus marmoreus No.1 is bred and harvested 10 days earlier, the average mushroom stem length is 14.4 cm,the average mushroom cover diameter is 1.4 cm, the average single-bottle yield is 330.5g, the average biological efficiency is 110.1%, the Hypsizygus marmoreus No.1 is superior to the parent originalstrain of the seafood mushroom and the crab mushroom, and the economic benefit is improved.

Description

technical field [0001] The invention relates to a true jiji mushroom strain and a breeding method thereof, and belongs to the technical field of edible fungus breeding. Background technique [0002] Shimeji mushroom ( Hypsizigus marmoreus ), also known as spot mushroom, jade mushroom, white jade, seafood mushroom, Hongxi mushroom, etc. It tastes fresher than oyster mushroom, tougher than shiitake mushroom in quality, thicker than slippery mushroom, and has a unique taste of crab. Its protein has a complete range of amino acids, including 8 kinds of essential amino acids for human body, among which the content of lysine and arginine is higher than that of common mushrooms. Since the 1980s, jiji mushrooms have been introduced to my country and started commercial planting, and have now become one of the important industrialized products of edible fungi in the country. In cultivation and production, its production cycle is longer (120-140d), the growth rate of the bacteria is ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/04C12R1/645
CPCC12N15/04C12N1/145C12R2001/645
Inventor 张积森王刚林婧娴窦梅杰王园园郭林常小光
Owner FUJIAN AGRI & FORESTRY UNIV
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