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Nandina domestica tissue culture and rapid propagation method based on LED (light-emitting diode) light sources

A flame Nandina, LED light source technology, applied in the field of reproduction, can solve the problems of long breeding cycle, slow reproduction speed of flame Nandina, few branches, etc., to achieve the effects of improving reproduction speed, good growth of new shoots, and cost saving

Active Publication Date: 2014-06-11
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the short stem nodes and few branches of the flame nandina, the propagation speed is too slow by the traditional cutting and branching methods, and it is difficult to form industrialized development. At present, the research on the tissue culture technology of flame nandina has just begun, and the propagation cycle is long , the problem of low reproduction coefficient has not been overcome, resulting in slow propagation speed of flame nandina and relatively lagging development speed, which seriously affects the industrialization development of flame nandina

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of flame nandina tissue culture rapid propagation method based on LED light source, comprises the following steps:

[0023] (1) Obtain the stem of flame nandina

[0024] Take flame nandina tissue culture seedlings, inoculate the separated lateral buds on the subculture medium, and place them under the green LED light for cultivation, the light time is 12h / d, and the light intensity is 40μmol / m 2 s; After 30 days of cultivation, each lateral bud differentiated into 4 new lateral buds, and the stem section of the lateral buds of Flame Nandina was extended to 4.2cm; among them, the dominant wavelength of the green LED light was 525nm, and the spectral range was 577-492nm.

[0025] (2) Proliferation and cultivation stage of flame Nantian bamboo stem segments

[0026] The method of combining ramet proliferation and stem segment proliferation is used for subculture propagation, and the stem is cut into 0.5cm stem segments, each stem segment has a side bud, whic...

Embodiment 2

[0033] A kind of flame nandina tissue culture rapid propagation method based on LED light source, it is characterized in that, comprises the following steps:

[0034] (1) Obtain the stem of flame nandina

[0035] Take the flame nandina tissue culture seedlings, inoculate the separated lateral buds on the subculture medium, and place them under the green LED light for cultivation, the light time is 12h / d, and the light intensity is 50μmol / m 2 s; After culturing for 35 days, each side bud differentiated into 5 new side buds, and the stem section of the side buds of Flaming Nandina chinensis elongated to 4.8cm;

[0036] (2) Proliferation and cultivation stage of flame Nantian bamboo stem segments

[0037] The method of combining ramet proliferation and stem section proliferation is used for subculture propagation. The stem is cut into 1cm stem sections, each stem section has a side bud, and is inoculated on the adventitious bud proliferation medium for proliferation and c...

Embodiment 3

[0044] A kind of flame nandina tissue culture rapid propagation method based on LED light source, it is characterized in that, comprises the following steps:

[0045] (1) Obtain the stem of flame nandina

[0046] Take the flame Nandina tissue culture seedlings, inoculate the separated lateral buds on the subculture medium, and place them under the green LED light for cultivation, the light time is 12h / d, and the light intensity is 70μmol / m 2 s; After culturing for 40 days, each side bud differentiated into 5 new side buds, and the stem section of the side buds of Flaming Nandina chinensis elongated to 5cm;

[0047] (2) Proliferation and cultivation stage of flame Nantian bamboo stem segments

[0048] The method of combining ramet proliferation and stem segment proliferation is used for subculture propagation, and the stem is cut into 0.8cm stem segments, each stem segment has a side bud, which is inoculated on the adventitious bud proliferation medium for proliferation...

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Abstract

The invention relates to a Nandina domestica tissue culture and rapid propagation method based on LED (light-emitting diode) light sources, and belongs to the technical field of propagation. The method is characterized in that the LED light sources with different light qualities are adopted for treatment in stages step by step, single-color green is firstly used as a Nandina domestica tissue culture light source to increase stem lengths and spacing between the stems of the Nandina domestica, then propagation is performed by adopting a stem propagation method, and a fluorescent lamp is used as a light source for stem culture. Compared with the prior art, the Nandina domestica tissue culture and rapid propagation method has the advantages that the Nandina domestica is propagated to 7-8 times, the growth period is shortened to 30-40 days, the situation that the Nandina domestica tissue culture growth cycle is long and the propagation rate is low is solved, the production cost is lowered, the propagation speed of Nandina domestica and the quality of seedlings are greatly increased, and a new method is provided for increasing the propagation speed of the Nandina domestica so as to provide guarantee for industrialization development of the Nandina domestica.

Description

technical field [0001] The invention relates to a method for tissue culture of garden plants, in particular to a method for tissue culture and rapid propagation of flame nandina based on an LED light source, and belongs to the field of propagation technology. Background technique [0002] Flaming Nandina belongs to the genus Nandina of the family Nandinaceae. The plant is short and compact, with oval or ovate leaves and extremely short internodes, only 1 / 10 of ordinary nandina. Flame nandina begins to germinate in mid-April in Shanghai area, and grows tender red new leaves. Big, when the temperature rises, it will gradually turn green above 30°C. In December, when the temperature drops below 5°C and the temperature difference between day and night is above 10°C, the leaves begin to turn red. Because it is cold-resistant and evergreen, it will not wither through winter and is very beautiful. , suitable for ground cover and small garden environment layout. The research on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王姗王永平许建民王全智鲍华鹏
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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