Construction method of agrobacterium tumefaciens-mediated transgenic plants

A technology of Agrobacterium rhizogenes and transgenic plants, which is applied in the field of plant genetic engineering, can solve problems such as long cycle times, and achieve the effects of simple equipment, no need for redifferentiation, and broad development and application prospects
CN109679993AActive Publication Date: 2019-04-26BEIJING FORESTRY UNIVERSITY
10 Cites 10 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2019-04-26

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a construction method of agrobacterium rhizogenes-mediated transgenic plants, and relates to the technical field of plant genetic engineering. The method comprises the steps offirstly, constructing agrobacterium rhizogenes with a target gene, enabling sterile seedlings of plants to take roots and then planting; when the stem lengths of the planted sterile seedling are morethan 3cm from the roots, injecting the bacterial liquid of the agrobacterium rhizogenes with the target gene into the stems of the sterile seedlings of the plants; after the transgenic hairy roots ofthe plants grow out at the injected parts of the stems, culturing until the lengths of the transgenic hairy roots are greater than 3cm and the number of roots is increased to 10, removing the non-hairy roots and extending the hairy roots into the soil to obtain the transgenic plants with the hairy roots. Compared with the conventional plant transgenic system, the method provided by the invention can omit the plant re-differentiation step of callus cells and overcome the problems of long period and the like which are caused by using the callus cells for genetic transformation. The method provided by the invention requires simple equipment and easy in mastering of operation technology, thus having a broad development and application prospects.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of plant genetic engineering, in particular to a method for constructing transgenic plants mediated by Agrobacterium rhizogenes. Background technique

[0002] Agrobacterium rhizogenes is a G-Agrobacterium with a broad host range. After Agrobacterium infects plants, it can induce plants to produce a large number of highly branched adventitious roots, commonly known as hairy roots. The hairy roots produced by Agrobacterium rhizogenes infecting plants have the characteristics of fast growth, high degree of differentiation, stable physiology, biochemistry and genetics, and easy operation and control.

[0003] In genetic engineering, Agrobacterium rhizogenes infection to form transgenic hairy roots is a fast and efficient transgenic method. In this study, a rapid and efficient apple transgenic system was established through Agrobacterium infection using the aseptic 'Gala' seedling of the same region, the same a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More