Adjuvant for animal vaccines, and preparation method and application thereof

A technology for vaccines and animals, which is applied in the field of adjuvants for animal vaccines and its preparation, and can solve problems such as becoming water-in-oil or oil-in-water, affecting the biological activity of antigens, and instability of water-in-oil-in-water , to achieve the effects of low oil density, unique slow-release effect, and rapid immune response

Active Publication Date: 2017-05-24
长沙国瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Secondly, the water-in-oil-in-water adjuvant and the antigen preparation process are also unstable. For biological safety considerations, the emulsifier used in the adjuvant is non-ionic, and the HLB value (hydrophilic-lipophilic balance value) of the emulsifier It is one of the keys to form the water-in-oil-in-water type. The HLB value of the non-ionic emulsifier will change with the temperature. If the temperature is not suitable, the water-in-oil-in-water type will be unstable and will become water-in-oil or water-in-oil. Oil-in-oil type, and at the same time, temperature will also affect the biological activity of the antigen, so temperature has become a difficult point in the production process and production equipment of water-in-oil-in-water vaccines. Selecting a suitable emulsifier can reduce the effect of emulsifier HLB value on temperature The sensitivity of the emulsifier is a shortcut to solve the problem, and the existing water-in-oil-in-water does not propose a solution to reduce the sensitivity of the emulsifier HLB value to temperature
[0005] 3. Ease of preparation: Two or more steps of emulsification for the preparation of water-in-oil-in-water adjuvant and antigen will bring cumbersome emulsification process and increase the emulsification process time, which will affect the biological activity of the vaccine

Method used

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  • Adjuvant for animal vaccines, and preparation method and application thereof
  • Adjuvant for animal vaccines, and preparation method and application thereof
  • Adjuvant for animal vaccines, and preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0053] A preparation method of the above-mentioned adjuvant for animal vaccines, comprising the following steps:

[0054] According to the volume fraction of the raw material components, first measure low-density mineral oil, then add caprylic capric acid glycerin and Span-80 to the low-density mineral oil, then add glycerin and polyglyceryl isostearate, and finally add poly Ethylene glycol-12-hydroxystearate-15, Tween-80 (can be 0) and propylene glycol, after mixing uniformly, leave standstill, after there is no air bubble in the obtained mixed solution, seal storage, obtain the adjuvant for animal vaccine agent;

[0055] Or, according to the volume fraction of the raw material components, first measure low-density mineral oil, then add caprylic capric acid glyceride and Span-80 to the low-density mineral oil, and then add polyethylene glycol-12-hydroxystearic acid Ester-15, Tween-80 (can be 0) and propylene glycol, and finally add glycerin and polyglyceryl isostearate, mix ...

Embodiment 1

[0059] A kind of animal vaccine adjuvant (GR208 adjuvant) of the present invention, comprises the raw material of following volume fraction:

[0060]

[0061] In this embodiment, the volume fraction of caprylic acid glyceride is 1%, and the volume fraction of Span-80 is 6%.

[0062] In this embodiment, the volume fraction of glycerin is 1%, and the volume fraction of polyglyceryl isostearate is 1%.

[0063] In this embodiment, the volume fraction of polyethylene glycol-12-hydroxystearate-15 is 1%, the volume fraction of Tween-80 is 3%, and the volume fraction of propylene glycol is 3%.

[0064] In this embodiment, the low-density mineral oil is specifically low-density medical-grade mineral oil, and its density at 15° C. is 0.834 kg / L.

[0065] In this embodiment, polyglyceryl isostearate is specifically polyglyceryl diisostearate.

[0066] In this example, the purity of Tween-80 was 99%.

[0067] A kind of preparation method of the animal vaccine adjuvant of above-menti...

Embodiment 2

[0097] A kind of animal vaccine adjuvant of the present invention comprises the raw material of following volume fraction:

[0098]

[0099]In this embodiment, the volume fraction of caprylic acid glyceride is 2%, and the volume fraction of Span-80 is 6%.

[0100] In this embodiment, the volume fraction of glycerin is 2.5%, and the volume fraction of polyglyceryl isostearate is 0.5%.

[0101] In this embodiment, the volume fraction of polyethylene glycol-12-hydroxystearate-15 is 4%, and the volume fraction of propylene glycol is 3%.

[0102] In this embodiment, the low-density mineral oil is specifically low-density medical-grade mineral oil, and its density at 15° C. is 0.84 kg / L.

[0103] In this embodiment, polyglyceryl isostearate is specifically polyglyceryl triisostearate.

[0104] A kind of preparation method of the animal vaccine adjuvant of above-mentioned present embodiment, comprises the following steps:

[0105] (1) According to the volume fraction of the abo...

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Abstract

The invention discloses an adjuvant for animal vaccines, and a preparation method and application thereof. The adjuvant for animal vaccines comprises the following raw materials by volume: 75 to 90% of low-density mineral oil, 1 to 10% of octyl and decyl glycerate and Span-80, 0.5 to 5% of glycerin and polyglycerol isostearate and 1 to 10% of polyethylene glycol-12-hydroxystearate-15, Tween-80 and propylene glycol, wherein the amount of Tween-80 is in a range of 0 to 5%. The preparation method comprises the following steps: weighing the low-density mineral oil; then adding octyl and decyl glycerate, Span-80, glycerin, polyglycerol isostearate, 1 to 10% of polyethylene glycol-12-hydroxystearate-15, Tween-80 and propylene glycol; and carrying out mixing and standing. The adjuvant provided by the invention has safety, stability and self-emulsifying performance, has low sensitivity to temperature in the process of emulsification, can form a water-in-oil-in-water spatial structure, and is extensively applicable to preparation of animal vaccines.

Description

technical field [0001] The invention belongs to the field of biological product animal vaccines, and in particular relates to an adjuvant for animal vaccines and a preparation method and application thereof. Background technique [0002] Currently, the two basic components of most animal vaccines are antigens and adjuvants. Adjuvants are like capsules of drugs, which can protect the vaccine and make the vaccine release slowly. Self-emulsification, two-step emulsification and multi-step emulsification will determine the difficulty of the vaccine production process and affect the biological activity of the vaccine. Water-in-oil type (W / O), oil-in-water type (O / W) and water-in-oil-in-water type (W / O / W) will determine the speed of animal immune response and the length of vaccine protection time, among which "water "Refers to the antigen, "oil" refers to the adjuvant, different types refer to the difference in the spatial structure of the oil and water package, and the water-in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/113
Inventor 刘卓灵
Owner 长沙国瑞生物科技有限公司
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