Method for inhibiting movement of flounder eyes

A flounder and eye technology, which is applied in the field of inhibiting flounder eye movement, can solve problems such as small natural mutation, and achieve the effect of obvious effect and single effect.

Inactive Publication Date: 2011-03-30
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the probability of natural mutations is usually very small, and it is difficult to find individuals with non-moving eyes for comparative research, while larvae soaked in thiourea cannot complete metamorphosis and still maintain the shape of larvae, and thyroid hormones may regulate the growth and development of related tissues , so the method of thyroid hormone suppression can not achieve the expected purpose

Method used

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  • Method for inhibiting movement of flounder eyes
  • Method for inhibiting movement of flounder eyes
  • Method for inhibiting movement of flounder eyes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The method of microinjecting colchicine to inhibit the movement of the right eye of flounder can be operated according to the following steps:

[0051] 1. Preparation of colchicine

[0052] Dilute the colchicine into a 5 mg / ml stock solution of colchicine with sterile 0.75% sodium chloride solution to prepare a 50 μg / ml colchicine solution.

[0053] 2. Preparation of anesthetics

[0054]Use freshly aerated seawater with a salinity of 35‰ and a temperature of 20°C to prepare a 0.1 mg / mL solution of ethyl 3-aminobenzoate methanesulfonate (tricaine methanesulfonate, MS-222), and prepare it immediately before use.

[0055] 3. Preparation of agar plates

[0056] Prepare a 2% agarose solution with distilled water, heat it in a microwave oven to dissolve, pour 15 to 20 mL of agarose solution into a 90 mm petri dish to condense for later use.

[0057] 4. Injection of colchicine

[0058] Microinjection of colchicine into flounder (Paralichthys olivaceus) larvae

[0059] (1)...

Embodiment 2

[0077] The method for microinjecting colchicine to inhibit the movement of the left eye of Sole oleracea can be operated according to the following steps:

[0078] 1. Preparation of colchicine

[0079] Dilute the colchicine into a 5 mg / ml stock solution of colchicine with sterile 0.75% sodium chloride solution to prepare a 50 μg / ml colchicine solution.

[0080] 2. Preparation of anesthetics

[0081] Use freshly aerated seawater with a salinity of 32‰ and a temperature of 18°C ​​to prepare a 0.1 mg / mL solution of ethyl 3-aminobenzoate methanesulfonate (tricaine methanesulfonate, MS-222), and prepare it immediately before use.

[0082] 3. Preparation of agar plates

[0083] Prepare a 2% agarose solution with distilled water, heat it in a microwave oven to dissolve, pour 15 to 20 mL of agarose solution into a 90 mm petri dish to condense for later use.

[0084] 4. Injection of colchicine

[0085] Microinjection of colchicine into Solea solea larvae

[0086] (1) Sole is raise...

Embodiment 3

[0099] The method of microinjecting colchicine to inhibit the movement of the right eye of tongue sole can be operated according to the following steps:

[0100] 1. Preparation of colchicine

[0101] Dilute the colchicine into a 5 mg / ml stock solution of colchicine with sterile 0.75% sodium chloride solution to prepare a 50 μg / ml colchicine solution.

[0102] 2. Preparation of anesthetics

[0103] Use freshly aerated seawater with a salinity of 32‰ and a temperature of 22°C to prepare a 0.1mg / mL solution of ethyl 3-aminobenzoate methanesulfonate (tricaine methanesulfonate, MS-222), and prepare it immediately before use.

[0104] 3. Preparation of agar plates

[0105] Prepare a 2% agarose solution with distilled water, heat it in a microwave oven to dissolve, pour 15 to 20 mL of agarose solution into a 90 mm petri dish to condense for later use.

[0106] 4. Injection of colchicine

[0107] Microinjection of colchicine into Cynoglossus semilaevis larvae

[0108] (1) Semi-sm...

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PUM

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Abstract

The invention discloses a method for inhibiting the movement of flounder eyes, which comprises the selection of an inhibiting drug, i.e. colchicine, the method and the skill of microinjection, and the position and the time for injecting paralichthys, solea and cynoglossus semilaevis. The method can restrict the action of the drug in the local tissues so as to achieve better action and effect. The movement of the flounder eyes is inhibited by injecting the colchicine, the generation of other metamorphosis events can be not influenced, and the method can provide the experimental materials to the research of the mechanisms asymmetrical occurrence.

Description

technical field [0001] The invention relates to a method for inhibiting eye movement of flounder, which comprises injecting colchicine into flounder larvae before metamorphosis, as well as the concentration and dose of colchicine, the injection method, the injection time and the injection site. It can be used to breed flounder whose eyes do not move but other metamorphosis events are normal, and to provide flounder with bilateral symmetry for the field of scientific research. Background technique [0002] Flounder is the general term for flatfishes. Both eyes are located on one side of the body. Among the bilaterally symmetrical vertebrates, the left-right asymmetry of flatfish is a special case. The establishment of body asymmetry in flatfish is crucial to the normal development of tissues and organs, and larvae that fail to establish asymmetry in production will die in large numbers. In addition, the establishment of body asymmetry is crucial to the normal development of ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 鲍宝龙柯中和邢巨斌龚小玲谢彩霞徐冰盖军伟
Owner SHANGHAI OCEAN UNIV
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