Preparation method of classical swine fever spleen-lymph-sourced compound living vaccine

A technology of composite live vaccine and swine fever splenic leaching source, which is applied in the field of veterinary biological products, can solve the problems of time-consuming and laborious, expensive vaccines, high production costs, etc., and achieve the effect of improving humoral immunity, improving immunity and improving protection rate

Inactive Publication Date: 2015-09-09
浙江美保龙生物技术有限公司
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AI Technical Summary

Problems solved by technology

[0010] The traditional antigen-antibody complex vaccine production method is to combine hyperimmune serum with bursal virus. This method requires a large amount of hyperimmune serum antibodies to be obtained by slaughtering a large number of animals, which is time-consuming and laborious. At the same time, the production cost is high, resulting in Vaccines are more expensive

Method used

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  • Preparation method of classical swine fever spleen-lymph-sourced compound living vaccine
  • Preparation method of classical swine fever spleen-lymph-sourced compound living vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the preparation of Chinese medicine polysaccharide and Chinese medicine flavone

[0047] a. According to 30 parts of weeping pot grass, 20 parts of golden fruit olive, 20 parts of chicken vine, 30 parts of buffalo horn, 30 parts of mole cricket, 10 parts of fennel, 10 parts of Fangji, 10 parts of lobelia and 10 parts of Passepartout Chinese medicine raw materials, chopped, washed, soaked in cold water overnight, then added purified water 15 times the weight of raw materials, water bathed to 90 ° C, and kept at 90 ° C, boiled for 2 hours, stirring every 10 minutes during the boiling process;

[0048] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0049] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, a...

Embodiment 2

[0055] Embodiment 2 The preparation of chicken anti-swine fever virus egg yolk antibody comprises the following steps:

[0056] a. Immune healthy laying hens with swine fever inactivated vaccine. The immunization program is the first chest muscle immunization 0.5ml / bird; the second immunization one week after the first immunization, chest muscle immunization 1.0ml / bird; the third immunization two weeks after the second immunization, chest muscle immunization 1.0ml / bird.

[0057] b. Two weeks after the third immunization, start to monitor the antibody titer of high-immunity eggs. When the agar diffusion titer of anti-porcine reproductive and respiratory syndrome virus yolk antibody in the high-immunity eggs reaches 1:64, collect qualified high-immunity eggs.

[0058] c. Separation of egg yolk: Clean and disinfect the undamaged high-free eggs collected in b with 0.1% bromogeramine, break the shell and separate the egg yolk, collect the egg yolk in the egg yolk storage tank, and ...

Embodiment 3

[0062] Embodiment 3: the preparation of a kind of lyoprotectant of swine fever spleen and drenching compound live vaccine

[0063] Add 60g of traditional Chinese medicine polysaccharide, 40g of traditional Chinese medicine flavonoids, 10g of vitamin combination (2g of folic acid, 3g of nicotinamide, 1g of vitamin B and 4g of vitamin E) into every 1000ml of phosphate buffer solution to obtain the lyoprotectant for live vaccine of swine fever spleen and gonorrhea . Wherein the Chinese medicine polysaccharide and the Chinese medicine flavone are prepared according to the steps in Example 1. Phosphate buffer solution consists of 6-10g sodium chloride, 0.05-0.5g potassium chloride, 1-1.2g disodium hydrogen phosphate, 0.05-0.5g potassium dihydrogen phosphate, 0.05-0.2g calcium chloride, containing 6 crystal water Dissolve 0.05-0.2g of magnesium chloride in 1000ml of double-distilled water and sterilize under high pressure at 116°C for 30 minutes.

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Abstract

The invention relates to a preparation method of a classical swine fever spleen-lymph-sourced compound living vaccine and belongs to the technical field of veterinary biological products. The preparation method of the classical swine fever spleen-lymph-sourced compound living vaccine comprises the following process steps: selecting a domestic rabbit to be inoculated; inoculating; measuring the temperature and observing; harvesting; preparing virus suspension and inspecting; carrying out mixed adsorption on the harvested full-suspension virus bulk and chicken anti-hog cholera virus egg yolk antibody; and adding freezing and drying protecting agent containing an immunopotentiator into antigen antibody compound obtained through adsorption, carrying out split charging, freezing, and carrying out vacuum drying, so that the classical swine fever spleen-lymph-sourced compound living vaccine is obtained. By adopting the preparation method of the classical swine fever spleen-lymph-sourced compound living vaccine, an antibody acquiring route is simple, animal welfare can be improved, manpower and material resources are saved, production cost is greatly reduced, humoral immunity of the living vaccine can be greatly improved, and high neutralizing antibodies can be produced; and the classical swine fever spleen-lymph-sourced compound living vaccine can be applied to piglet immunization, maternal antibody interference can be avoided, no interference is produced to other vaccines, especially a mycoplasma hyopneumoniae vaccine and the like, after the classical swine fever spleen-lymph-sourced compound living vaccine is applied to piglets, and strong mucosal immune response can be induced, so that protection rate is improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a composite live vaccine derived from swine fever, spleen and leprosy. Background technique [0002] Classical swine fever (CSF) is a Class A legal infectious disease of the International Organization for Animal Health (OIE) and China. It is one of the major animal diseases that are subject to compulsory immunization in my country, and is the "first killer" that endangers my country's pig industry. Swine fever is caused by swine fever virus and cannot be controlled with drugs. Vaccination is the most effective way. [0003] At present, the CSF vaccines on the market at home and abroad mainly include CSF splenic vaccine, CSF cell vaccine and CSF subunit vaccine. As a classical-refined live vaccine of classical swine fever and splenic gonorrhea in vaccines, it still has an irreplaceable role. [0004] The Chinese swine fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K39/187A61K39/39A61P31/14A61K36/74A61K35/64A61K35/32A61K31/519A61K31/455A61K31/355
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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