The invention provides a cell apoptosis inducer, a drug-loaded vesicle and application thereof, and belongs to the technical field of biology. The apoptosis inducer comprises an effective amount of yeast mannan and an effective amount of calcium ions. The yeast mannan is used as a calcium ion carrier, the permeability of cell membranes to the calcium ions can be improved, high-concentration free calcium ions can be gathered in cells within a short time, then a cell apoptosis procedure is triggered, cell vesicles are generated, and the obtained cell vesicles are high in yield, good in uniformity and small in average cell vesicle diameter. When the cell vesicle is used for wrapping a drug, the cell vesicle can efficiently pass through the barrier of tumor cells and is endocytosed by the tumor cells, so that the drug delivery efficiency is improved.