15 results about "Cell vesicle" patented technology

The invention discloses a Vismodegib and BMS-1 co-loaded bionic nano delivery system as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the preparation method disclosed by the invention, Vismodegib and BMS-1 are prepared into a nano-micelle core, and then red blood cell vesicles are utilized to prepare the bionic nano delivery system co-loaded with Vismodegib and BMS-1. Experiments prove that the bionic nano delivery system prepared by the invention inhibits the growth of mouse in-situ tumors and the formation of lung / liver tumor metastasis lesions, and the effect is obviously superior to that of Vismodegib or BMS-1 used alone, Vismodegib and BMS-1 combined use and Vismodegib or BMS-1 loaded nano delivery carriers. The invention provides a new idea for clinical treatment of triple negative breast cancer.

The invention discloses a preparation method and application of an IP10-fusion cell vesicle microneedle vaccine with a T-cell activation function, IP10-fusion cell vesicles with the T-cell activation function are prepared into the microneedle vaccine, on the immune activation level, the IP10-fusion cell vesicles have a vesicle antigen presentation function through the chemotactic effect of IP10, and the T-cell activation function of the IP10-fusion cell vesicles is achieved. T cell-mediated anti-tumor immune response can be efficiently excited, and a new path is provided for solving the problem of tumor immune escape; in the aspect of a delivery system, the minimally invasive slow release characteristic of the microneedle not only reduces the acceptance resistance of a patient, but also can maintain lasting immunostimulation, and compared with a traditional injection mode, the compliance and the treatment effect of the vaccine are remarkably improved. In addition, good biocompatibility ensures the safety of clinical application, and has great transformation potential in the field of tumor prevention and treatment.

The invention discloses a plant cell vesicle and a preparation method thereof, and belongs to the technical field of biology. The preparation method comprises the steps of plant tissue pretreatment (adding cane sugar as an osmotic pressure protective agent), high-pressure homogenization (100-300MPa, 2-3 times), composite enzymolysis (mixing cellulase and pectinase, 35-45 DEG C, 10-30 minutes), differential centrifugation and ultracentrifugation (100,000 * g, 2 hours) combined purification. The average particle size of the obtained vesicle is 50-200nm, and the vesicle can be used for drug molecule or nucleic acid delivery. By optimizing parameters and additives in each step, the yield, structural integrity and functional characteristics of the vesicles are remarkably improved, and technical support is provided for application of the plant-derived vesicles in the field of nano-drug delivery.

The invention relates to the field of cosmetics and biotechnology thereof, in particular to a vesicle composition with a corn germ extract wrapped by black orchid cells as well as a preparation method and application of the vesicle composition. The vesicle composition disclosed by the invention is obtained by feeding a corn germ extract into a fermentation system of black orchid cells. The culture conditions of the fermentation system of the black orchid cells are as follows: the temperature is 22-25 DEG C, the speed is 120-180 rpm, and the culture period is 15-20 days. The corn germ extract can be added in a delay phase, an exponential phase, a later exponential phase and a plateau phase of fermentation culture of black cymbidium cells. The black cymbidium cell vesicle composition obtained by the invention has the advantages of low immunogenicity, enhanced cellular uptake, higher stability and targeting ability, and better affinity with a phospholipid bilayer of a skin cuticle. And after reaching the cuticle of the skin, active matters can be quickly released, so that the effects are achieved. The compound can be used for preparing antioxidant or anti-aging medicines or cosmetics.

The invention relates to an engineered cell vesicle, a preparation method thereof and application of the engineered cell vesicle in preparation of antitumor drugs, and belongs to the technical field of biological medicines. The invention provides a BITE engineered cell vesicle system, the BITE engineered cell vesicle system comprises a core layer and a shell layer coating the core layer, the shell layer is a vesicle loading PD-1 protein, the core layer is a CD47 gene knockout system, and the CD47 gene knockout system contains an expression vector for knocking out a CD47 gene. According to the BITE engineered cell vesicle system disclosed by the invention, targeted immune checkpoint blocking and a gene editing technology are combined, and targeted delivery and enrichment of a CD47 gene editing system in a vesicle in a tumor cell are realized through specific binding of PD-1 protein on the surface of the vesicle and PD-L1 on the surface of the tumor cell. The system can effectively knock out the CD47 gene in tumor cells, and realizes a targeted and synergistic cancer immunotherapy effect.

The invention relates to a recombinant adenovirus nucleic acid wherein the gene encoding protein V and / or the gene encoding protein VII is placed under control of a heterologous promoter, to a recombinant adenovirus nucleic acid wherein the adenoviral nucleotide sequence is mutated in such a way that it is no longer capable of producing one or more of the coat proteins, to cellular vesicles filled with such adenoviral material, cells provided with such adenoviral material and to methods and use thereof.

The invention provides a polygonum cuspidatum vesicin composition, the composition contains a polygonum cuspidatum root extract, resveratrol, an alcohol solvent and water, and the resveratrol is wrapped in polygonum cuspidatum cell vesicles after ultrasonic treatment. The average particle size of the prepared polygonum cuspidatum cell vesicles is in a preferable range, the particle size is uniform, the encapsulation efficiency and the drug loading capacity of resveratrol are high, and the number of vesicle particles is far beyond the standard. The composition is stable in physical property, soluble in water, good in safety and stable in performance. In the aspect of application, the composition has good bacteriostasis and biofilm inhibition effects on periodontal pathogenic bacteria, and anti-inflammation and tissue repair are realized through cooperation of multiple components. The invention solves the problems of water dispersibility, stability and absorptivity of resveratrol, has multiple advantages, and has wide application prospects in the fields of oral care and the like.

Described herein are compositions comprising "amphiphilic click reactive anchors" (ACRAs) that efficiently incorporate into natural and synthetic membranous structures on cells, vesicles, liposomes, lipid nanoparticles, and other materials, and methods of use thereof, e.g., for biological and chemical analysis, drug delivery, and imaging.

The invention provides application of erythrocyte vesicles in preparation of drugs for treating skin-related diseases. The erythrocyte vesicles (especially freeze-dried RBC-EVs) have the effect of treating skin-related diseases for the first time, and can treat acne, resist skin aging and treat androgenetic alopecia. Compared with a traditional medicine, the RBC-EVs serving as a natural biological agent has the advantages of biocompatibility and targeted delivery, side effects of the medicine can be reduced, and treatment safety can be improved. In addition, the red blood cell vesicle has a natural vesicle membrane structure, and is high in biocompatibility and low in immunogenicity. Vesicle therapy corrects disease disorder through core mechanisms such as immune regulation, collagen regeneration and intelligent delivery, and has the advantages of high safety (no cellular risk) and accuracy (engineering transformation can be realized).

The invention provides an exosome extraction method and prepared exosome freeze-dried powder, and belongs to the technical field of exosome extraction. The invention provides an exosome extraction method which comprises the following steps: firstly, performing high-temperature stimulation on cells to improve secretion and release of exosomes; then the blood platelets subjected to high-temperature stimulation are subjected to circulating freeze thawing, so that most of cells, vesicles and the like are broken, and the integrity of the target product exosome is reserved; and filtering to remove impurities such as cell debris, apoptotic bodies and the like, and retaining an exosome product of 50-150nm. The invention also provides exosome freeze-dried powder which is prepared by freeze-drying the exosome product, and the purity of the exosome freeze-dried powder can reach 70-80%. By utilizing the exosome extraction method disclosed by the invention, the capacity of the exosome can be remarkably improved, and the method disclosed by the invention can be used for removing most impurities and retaining the integrity and activity of the exosome, so that the purity of the prepared exosome product is improved.

The invention discloses a preparation method of polygonum cuspidatum cell vesicles and application of the polygonum cuspidatum cell vesicles in the aspect of inhibiting periodontal pathogenic bacteria. According to the method, the polygonum cuspidatum cell vesicles rich in target secondary metabolites are efficiently obtained through the steps of optimizing raw material pretreatment, preliminary centrifugal separation, tangential flow filtration and concentration, selective precipitation, precipitate collection and purification, gel adsorption and purification and the like. In the preparation process, different material-liquid ratios and buffer solution systems are adopted for dry or fresh polygonum cuspidatum raw materials, and stable extraction of target components is ensured. In addition, the invention further provides a preparation method of the polygonum cuspidatum vesicin, and the biological activity of the polygonum cuspidatum vesicin is remarkably enhanced by loading active substances such as resveratrol in vesicles. The polygonum cuspidatum vesicular element shows an efficient inhibition effect on periodontal pathogenic bacteria in an in-vitro bacteriostasis experiment, has anti-inflammatory and tissue repair functions, can be applied to various oral care products such as toothpaste and oral patches, and has a wide industrial application prospect.

The application provides a photodynamic anti-infection artificial stem cell vesicle hydrogel for promoting wound repair, which comprises a hydrogel carrier and AIE vesicles loaded in the hydrogel carrier; the AIE vesicles are AIE molecules wrapped with artificial stem cell vesicles on the outer surface; the mass ratio of the AIE molecules to the artificial stem cell vesicles ranges from 1:3 to 50, and the maximum final concentration of the AIE molecules in the AIE vesicles is not more than 400 micromoles per liter. The hydrogel not only has excellent anti-infection, antibacterial, repair-promoting regulation functions and good biological safety, but also has good stability and long-acting property. In addition, the hydrogel is simple to prepare and low in cost, has good universality, and has good practical popularization significance in the treatment of various infections.

The present application relates to a kind of preparation methods of engineering macrophage extracellular vesicle delivery system, the preparation method: obtaining cell strain of stable expression PD-L1 protein;Preparation cell vesicle;The cell vesicle is co-incubated with amphiphilic PEGylated phospholipid (PEGylated phospholipids) solution, and engineering macrophage extracellular vesicle delivery system is prepared.The engineering macrophage extracellular vesicle delivery system prepared in the present application can accurately regulate the immune balance between Tfh / Treg cell in spleen, to realize from the whole body immune regulation of peripheral to local, it can be used for treating RA.

This invention discloses a method for preparing and applying cell vesicles derived from the rhizomes of traditional Chinese medicinal plants. The preparation method includes the following steps: washing and crushing the rhizomes of the medicinal plants, adding PBS buffer and grinding to obtain a homogenate; centrifuging the homogenate differentially and collecting the supernatant; subjecting the supernatant to a first ultracentrifugation to obtain a crude extract of cell vesicles; resuspending the crude extract of cell vesicles and loading it into a discontinuous sucrose density gradient solution for a second ultracentrifugation; collecting gradient bands with buoyant densities between 1.13 g / mL and 1.19 g / mL. This invention provides a stable and scalable preparation process that, through differential centrifugation combined with sucrose density gradient ultracentrifugation, can efficiently separate high-purity, structurally intact cell vesicles from the rhizomes of traditional Chinese medicinal plants such as Bletilla striata, Polygonatum sibiricum, and Curculigo orchioides. The process has good reproducibility and avoids the ethical and viral contamination risks associated with mammalian-derived vesicles.

The invention belongs to the technical field of biological medicine, and discloses a sensitization radiotherapy auxiliary preparation and a preparation method and application thereof.The sensitization radiotherapy auxiliary preparation is biomineralization aging red blood cell vesicles, and the biomineralization aging red blood cell vesicles are formed by loading calcium phosphate layers on aging red blood cell vesicles. The process of loading the calcium phosphate layer by the aged red blood cell vesicles is biological mineralization, and specifically, the biological mineralization is that calcium ions in calcium chloride are chelated by acid residues on an aged red blood cell membrane, so that a calcium phosphate layer (CaP layer for short) is deposited on the surface of the vesicles; the senescent red blood cell vesicles are prepared by taking whole blood of mammals, adding an H2O2 solution to induce red blood cell senescence, and further preparing the required red blood cell vesicles. The sensitization radiotherapy auxiliary preparation provided by the invention is simple to prepare and high in biocompatibility, and can effectively improve the immune limitation of TME on tumors after LDRT, hinder the survival and metastasis of tumor cells and significantly inhibit tumor growth.