Preparation method of human hair melanosomes derivative and application of human hair melanosomes derivative in antibiosis and tissue repair

A derivative and human technology, applied in the field of biochemistry, can solve the problems of no photothermal therapy, achieve excellent biodegradability and biocompatibility, promote tissue repair, and simple operation

Pending Publication Date: 2021-03-19
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no suitable photothermal therapy (PTT)

Method used

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  • Preparation method of human hair melanosomes derivative and application of human hair melanosomes derivative in antibiosis and tissue repair
  • Preparation method of human hair melanosomes derivative and application of human hair melanosomes derivative in antibiosis and tissue repair
  • Preparation method of human hair melanosomes derivative and application of human hair melanosomes derivative in antibiosis and tissue repair

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Effect test

Embodiment 1

[0041] The preparation method of HHMs,

[0042] Pretreatment: Break human hair to a length less than 3cm, ultrasonically clean it with deionized water for 15min, the ultrasonic frequency is 100Hz, and the time is 15min, repeat washing three times, and dry naturally at room temperature;

[0043] Hydrothermal reaction: In a high-pressure hydrothermal kettle, the pretreated human hair is subjected to a hydrothermal reaction in a NaOH solution, the concentration of the NaOH solution is 0.5mol / L, and the amount of the solution is 30 times the mass of the human hair, The temperature of the hydrothermal reaction is 50°C, and the reaction time is 12h;

[0044] Separation: The material obtained after the hydrothermal reaction is completed is subjected to centrifugation, and the centrifugation speed is 9500pm, and the time is 15min. After centrifugation, remove the supernatant, wash with deionized water, and centrifuge again, repeating three times. Then the sample was vacuum-dried at ...

Embodiment 2

[0047] Determine the maximum amount of lysozyme (Lyso) loaded on HHMs via electrostatic interactions:

[0048] Specifically, 4 mg of the prepared HHM powder was uniformly dispersed in 5 mL of deionized water. Meanwhile, 4, 8, 16, 32, 64 mg of Lyso powder were dissolved in 5 mL of deionized water, and then mixed with the above HHMs solution. The mixed solution was stirred at room temperature for 6 hours and centrifuged at 9500 rpm for 15 minutes to obtain supernatant and HHMs-Lyso. HHMs-Lyso was ultrasonically washed with deionized water and centrifuged at 9500 rpm for 15 min, repeated three times. The corresponding supernatant was collected, and the free Lyso content in the supernatant was measured by a spectrophotometer at 281 nm using a UV-Vis spectrophotometer (UV-3600, Shimadu, JP). Therefore, the Lyso loading on HHMs can be calculated from the initial Lyso content and the final free Lyso content to be 1:0.63 (HHM:Lyso, mass ratio).

Embodiment 3

[0050] Preparation of HHMs-Lyso composites:

[0051] The prepared 30 mg HHMs powder was uniformly dispersed in 10 mL deionized water. Meanwhile, 10 mg of Lyso powder was dissolved in 10 mL of deionized water, and then mixed with the above HHMs solution to obtain a HHMs-Lyso solution (2 mg / mL). The HHMs-Lyso solution was stirred at room temperature for 6 hours, ready to use without storage.

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Abstract

The invention discloses a preparation method of human hair melanosomes derivatives (HHMs). The preparation method comprises the following steps of step 1, pretreatment: crushing, cleaning and drying human hair; step 2, hydrothermal reaction: carrying out hydrothermal reaction on the pretreated human hair in a NaOH solution; and 3, separation: carrying out centrifugal separation and vacuum drying on a solid sample material obtained after the hydrothermal reaction to obtain HHMs. The invention relates to a preparation method of a human hair melanosomes derivative-lysozyme (HHMs-Lyso) composite material. The preparation method comprises the following steps of uniformly dispersing prepared HHMs into deionized water to obtain a first feed liquid; dispersing lysozyme into deionized water to obtain second feed liquid; and mixing the first feed liquid and the second feed liquid in an isopyknic manner to obtain the HHMs-Lyso. According to the HHMs prepared through the method, MRSA can be efficiently killed by loading Lyso, and meanwhile tissue repair can be promoted through the HHMs.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a preparation method of a human hair melanosome derivative and its application in antibacterial and tissue repair. Background technique [0002] Diseases associated with bacterial infections are a growing threat to global healthcare and are a major contributor to global morbidity and mortality. For example, approximately 15 million people die each year from bacterial infections and their ensuing complications. In addition, the long-term misuse of antibiotics has driven the rapid evolution, growth and spread of antibiotic-resistant bacteria at an alarming rate, and infections associated with drug-resistant bacteria have emerged rapidly, resulting in huge public medical and economic burdens. In particular, the increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) strains in chronic or recurrent infections will present a major challenge to glo...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K38/47A61P17/02A61P31/04
CPCA61K41/0052A61K38/47A61P17/02A61P31/04C12Y302/01017A61K2300/00
Inventor 吴水林李浚刘想梅崔振铎杨贤金
Owner TIANJIN UNIV
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