A kind of biomimetic bismuth nanoflower and its preparation method and application

A bismuth nanometer, bionic technology, applied in the field of medicine, can solve the problems of low soft tissue contrast, nephrotoxicity, short contrast time, etc., and achieve the effects of enhanced uptake, strong synergy, and improved stability

Active Publication Date: 2022-06-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, CT (X-ray computed tomography imaging, CT) is the most important and commonly used imaging examination method in diagnosis and treatment at present. It can detect small nodules with a diameter of about 2 mm, but its biggest disadvantage is the low soft tissue contrast. Contrast agents need to be used to obtain high-resolution CT images. The contrast agents commonly used in clinical practice are mainly small molecule iodine contrast agents, but iodine contrast agents have nephrotoxicity and short contrast time.

Method used

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  • A kind of biomimetic bismuth nanoflower and its preparation method and application
  • A kind of biomimetic bismuth nanoflower and its preparation method and application
  • A kind of biomimetic bismuth nanoflower and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0041] Synthesis and Characterization of Bismuth Nanoflowers (BNFs)

[0042] (1) Weigh 2 g of PVP (manufacturer: Aladdin, model K29, average molecular weight: 58000), measure 50 mL of ethylene glycol, and dissolve it by ultrasonic for later use. Weigh 300 mg of bismuth nitrate pentahydrate, add 1 mol / L HNO 3 The solution was 10 mL, fully dissolved, NaOH 10 mg was added, ultrasonically dissolved, and then the prepared PVP solution was added. After thorough mixing, it was transferred to the reaction kettle and reacted at 150 ° C for 4 h. After cooling to room temperature naturally, the precipitate was collected by centrifugation (10,000 rpm, 40 min), washed three times to obtain milky white bismuth oxide nanoparticles, which were redispersed in 50 mL of purified water for use;

[0043] (2) Weigh 15 mg of sodium borohydride, measure 10 mL of pre-cooled purified water, and add bismuth oxide nanoparticle solution immediately after dissolving, and observe that the color of the solu...

Embodiment 2

[0046] Comparison of Photothermal Conversion Capability of Several Bismuth Nanomaterials

[0047] The bismuth nanospheres (SBNP), rhombic bismuth nanoparticles (RBNP), hollow bismuth nanoparticles (HBNP), and the bismuth nanoflowers (BNF) synthesized by the present invention are prepared according to the method of relevant literature, and the bismuth content is adjusted by centrifugation and concentration. same.

[0048] Take 1 mL of the above solution into a quartz dish, respectively, and 808 nm and 1064 nm lasers at 3w / cm 2 Irradiate for 10min, the infrared thermometer detects the temperature every 1min, and use PBS as a control, the results are as follows image 3 . The power is 3 W / cm 2 After 10 min of continuous laser irradiation, the heating capacity of BNF at dual wavelengths was significantly higher than that of SBNP, RBNP and HBNP, and BNF was the fastest heating nanomaterial among the four nanoparticles.

Embodiment 3

[0050] Preparation of erythrocyte membrane-modified bismuth nanoflowers (RBCM-BNF)

[0051] Erythrocyte Membrane Protein 4 mg

[0052] DSPE-PEG 2000 32 mg

[0053] Bismuth Nanoflowers 16 mg

[0054] It was prepared by the following method: take an appropriate amount of freeze-dried cell membrane, measure the content of membrane protein with BCA kit, add an appropriate amount of PBS in ice bath and ultrasonicate for 30 s to fully disperse, then add DSPE-PEG in prescription amount 2000 Cell membrane vesicles were obtained by passing through 400 nm and 200 nm carbonate membranes for 5 times. The prescription amount of bismuth nanoflower solution was added, and the bismuth nanoflowers modified by red blood cell membrane were obtained by passing through the carbonate film of 400 nm and 200 nm for 5 times in turn.

[0055] The products were detected using transmission electron microscopy and UV full-wavelength scanner, and the results were as follows figure 1 (B) and figure ...

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Abstract

The invention discloses a bionic bismuth nanoflower, a preparation method and application thereof, and belongs to the technical field of medicine. The biomimetic bismuth nanoflowers include cell membranes and bismuth nanoflowers, and the cell membrane is used as a shell to cover the surface of the bismuth nanoflowers. The biomimetic bismuth nanoflowers of the present invention not only have good biological safety and degradability in the physiological environment, but also have good NIR II photothermal conversion efficiency, radiotherapy sensitization and CT / PAI dual-mode imaging, and are integrated photothermal synergistic radiotherapy in A new integrated carrier for diagnosis and treatment.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a bionic bismuth nanoflower, a preparation method thereof, and an application in the preparation of a diagnosis and treatment carrier. Background technique [0002] Photothermal therapy (PTT) is a method of treating tumors by artificially increasing the temperature of human tissue. It has been intensively studied in both preclinical and clinical studies due to its high efficiency, minimal invasiveness, and high selectivity. Energy is absorbed by tissue and converted into heat, causing irreversible tissue damage. Although hyperthermia cannot replace surgery, radiotherapy or chemotherapy as an independent treatment plan, it has obvious synergistic and complementary effects on chemotherapy, radiotherapy and surgery. [0003] Interventional radiology (IR) is a treatment method that uses high doses of radiation to kill cancer cells and inhibit tumor growth. Radiosensitiz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/50A61K47/46A61K49/04A61K49/22A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K41/0052A61K9/5068A61K49/0002A61K49/04A61K49/225A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00A61K41/0038A61K2300/00
Inventor 沈雁王璐闻静
Owner CHINA PHARM UNIV
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