Method for preparing natural L-alpha-glycerol phosphatidylcholine

A technology of glycerol phosphatidylcholine and phosphatidylcholine, which is applied in the field of preparation of natural L-α-glycerol phosphatidylcholine, can solve problems such as the danger of using solvent ether, the safety of products, and the toxicity of catalysts, etc. The effect of short time, low toxicity and mild conditions

Inactive Publication Date: 2013-03-13
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems existing in the existing GPC process of using natural lecithin as raw material, the catalyst is poisonous, the solvent ether is dangerous to use, and the reactant methanol has potential safety hazards to the process and products, etc.

Method used

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  • Method for preparing natural L-alpha-glycerol phosphatidylcholine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Dissolve 5 g of soybean lecithin with a phosphatidylcholine content of 40% in 38 mL of methyl tert-butyl ether, filter off the insoluble matter, add it to a round bottom flask and place it in a constant temperature water bath and heat it to 40°C. Next, 1.2 mL of absolute ethanol and 0.8 mL of ethanol solution of choline hydroxide with a concentration of 0.3 mol / L were added, and the reaction was stirred for 2 hours.

[0027] After the reaction, pour off the supernatant, dissolve the oily precipitate with 5mL of anhydrous methanol, and add 20mL of methyl tert-butyl ether for precipitation. After the precipitation is complete, discard the supernatant, and repeat 3 times to dissolve with methanol and use The operation of the precipitation of methyl tert-butyl ether, finally the obtained oily product is vacuum-dried in the presence of phosphorus pentoxide to obtain 1.021g of natural phospholipid deacylation (GPC) with light yellow appearance as the main GPC , a mixture of G...

Embodiment 2

[0029] The difference between this example and Example 1 is that 5 g of soybean lecithin with a phosphatidylcholine content of 60% is dissolved in 30 mL of methyl tert-butyl ether, the insolubles are filtered off, and the round bottom flask is added and placed in a constant temperature water bath and heated to 50° C., and at this temperature, 8 mL of absolute ethanol and 2 mL of choline hydroxide ethanol solution with a concentration of 0.3 mol / L were added, and the reaction was stirred for 1 hour. The present embodiment obtains 1.086g of the natural phospholipid deacyl substance (i.e. a mixture of GPC, GPE and GPS) whose appearance is light yellow, mainly based on GPC. After testing, the content of GPC accounts for 73.2% of the total product mass, and the yield is 78.4%.

Embodiment 3

[0031]The difference between this embodiment and Example 2 is that the content of phosphatidylcholine in soybean lecithin is 95%, the reaction temperature is 40°C, and the reaction time is 3h. After the reaction, dissolve the oily precipitate at the bottom with 5mL of anhydrous methanol, then add 20mL of methyl tert-butyl ether for precipitation, discard the supernatant after the precipitation is complete, repeat 3 times of dissolving with methanol and precipitate with methyl tert-butyl ether Then, the product was vacuum-dried in the presence of phosphorus pentoxide, and in the presence of diatomaceous earth, using a mixture of absolute ethanol and ether with a volume ratio of 6:4 at 4°C Crystallization was carried out to obtain 1.026 g of GPC crystals. Melting point 133°C, (10% aqueous solution), the elemental analysis results are: C, 37.1%; H, 7.80%; N, 5.43%. With deuterated methanol as solvent, 1 The positions of the main peaks in the H NMR spectrum: 4.36(m,2H), 3.91(m...

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Abstract

The invention discloses a method for preparing natural L-alpha-glyceryl phosphatide dipalmitoyl phosphatidyl choline. The method comprises the following steps of: dissolving natural lecithin having the known content of phosphatidylcholine into methyl tertiary butyl ether; filtering out undissolved substances and regulating the temperature to 5-55 DEG C; adding anhydrous low carbon alcohol and hydroxide choline into mixing liquid in a molar rate of anhydrous low carbon alcohol to phosphatidylcholine of (3:1)-(600:1)_to stir and react for 0.25-4 hours; pouring out supernate; dissolving oily precipitates with absolute ethyl alcohol and precipitating the oily precipitates by methyl tertiary butyl ether; removing the supernate after the precipitation is totally finished; repeatedly dissolving the precipitates by the absolute ethyl alcohol and precipitating the precipitates by the methyl tertiary butyl ether for 2-3 times; vacuumizing and drying obtained oily products. By the method, transesterification between the phosphatidylcholine and the anhydrous low carbon alcohol is carried out by using hydroxide choline as a catalyst and the methyl tertiary butyl ether as a solvent so as to obtain glycerol phosphatidylcholine (GPC) or natural lecithin deacylation substances using the GPC as a main part; and moreover, the method is simple in process, mild and safe in reaction conditions, and can guarantee the safety of the obtained products used as functional food addictives or medical health-care products.

Description

technical field [0001] The invention relates to the fields of medicine, functional food industry and catalytic technology, in particular to a preparation method of natural L-alpha-glycerol phosphatidylcholine. Background technique [0002] L-α-glycerophosphocholine (L-α-glycerophosphocholine, hereinafter referred to as GPC) is a unique water-soluble phospholipid naturally occurring in the human body. Studies have shown that GPC can not only improve the memory ability and cognitive ability of people at different stages, especially for the elderly, it can effectively prevent and treat Alzheimer's disease, and has a certain inhibitory effect on the cerebral apoplexy of the elderly; at the same time, it also It can promote the release of human growth hormone, improve the height of teenagers to a certain extent, and can also effectively regulate the hormone balance of middle-aged people, and have the effect of beautifying and slowing down aging. Therefore, GPC can be widely used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/09
Inventor 张小里李红亚梁江张希双赵彬侠
Owner NORTHWEST UNIV(CN)
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