Method for extracting polyoses from bagasse

A technology of bagasse and polysaccharides, which is applied in the field of preparation of polysaccharides, can solve problems such as difficulty in obtaining good results, and achieve the effects of less solvent consumption, high extraction rate, and short extraction time

Inactive Publication Date: 2010-07-21
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Ganoderma lucidum is a fungus, and the polysaccharides of Ganoderma lucidum are mostly stored in the cell wall of the fruiting body. The main components of the fruiting body are cellulose, hemicellulose and lignin, while sugarcane is a plant, and bagasse is mainly composed of whole cellulose, lignin, polysaccharides, etc. It is composed of pentosan, etc., and its fibers mainly include true fiber and sugarcane pith. True fiber has a relatively tough hard wall tissue, and sugarcane pith is like a spongy structure, which is quite different from Ganoderma lucidum. It is difficult to obtain it by applying the above method. Good results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 1Kg of bagasse coarse powder, add 8 liters of pure ethanol, reflux and extract for 1 hour, filter and collect the filter residue. Add 10 times of water, add 15 grams of cellulase, adjust the pH to 5.5, mix with the filter residue, keep at 60°C for 60 minutes, sonicate for 60 minutes, and filter. The filtrate is a polysaccharide extract. After the extract is concentrated, add ethanol to adjust the alcohol The concentration is 90%, placed overnight, the precipitate is vacuum dried at 60°C, and crushed to obtain 85 g of the crude bagasse polysaccharide.

Embodiment 2

[0019] Add 1Kg of bagasse coarse powder, add 10 liters of 90% ethanol, reflux and extract for 2 hours, and collect the filter residue by filtration. Add 12 times of water, add 5 grams of cellulase, adjust the pH to 4.0, mix with the filter residue, keep at 80°C for 30 minutes, sonicate for 90 minutes, filter, the filtrate is the polysaccharide extract, after the extract is concentrated, add ethanol to adjust the alcohol The concentration is 85%, and it is left overnight, the precipitate is vacuum dried at 70°C, and crushed to obtain 65 g of the crude bagasse polysaccharide.

Embodiment 3

[0021] Add 1Kg of bagasse coarse powder, add 10 liters of 90% ethanol, reflux and extract for 2 hours, and collect the filter residue by filtration. Add 12 times of water, add 10 grams of cellulase, adjust the pH to 6.0, mix with the filter residue, keep at 40°C for 90 minutes, sonicate for 30 minutes, and filter. The filtrate is polysaccharide extract. After the extract is concentrated, add ethanol to adjust the alcohol. The concentration is 85%, the precipitate is left overnight, and the precipitate is vacuum dried at 70° C., and crushed to obtain 64 g of the crude bagasse polysaccharide.

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PUM

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Abstract

The invention provides a polyhexose extraction method from the sugar cane bagasse. The method is realized by that after the degreasing processing is performed to the sugar cane bagasse, one or more of cellulolytic enzyme, papayotin and pectolytic enzyme can be hysrolyzed for 0.5 to 1.5 hours under pH value of 4 to 6 at the temperature of 40 to 80 DEG C, treated for 0.5 to 1.5 hours under the supersonic wave with the frequency of 59KHz, and filtered, and finally the polyhexose in the filtrate water is precipitated through ethanol. The method of the invention adjusts the types and the dose of the enzyme on the technology base of eumycete polyhexose extraction, and finds a low energy consumption and efficient sugar cane bagasse polyhexose extraction method under the enzyme processing and supersonic wave extraction technology.

Description

Technical field [0001] The invention relates to the preparation of polysaccharide compounds, in particular to the extraction of bagasse polysaccharides. Background technique [0002] Bagasse is the main by-product of the sugar industry. It is the main part of the sugarcane after mechanical compression. It is a kind of agricultural solid waste. Its composition is similar to that of wood materials and can be used as a raw material to replace part of the wood. my country's Hainan, Guangdong, Guangxi, Fujian, Yunnan and other provinces can grow sugarcane and are also important sugar-producing provinces. Each sugar factory stocks a large amount of bagasse, so it is of great significance to find a suitable way to comprehensively utilize bagasse. [0003] Bagasse contains polysaccharides, mainly composed of five-carbon sugar and six-carbon sugar. Studies have shown that the polysaccharides extracted from bagasse have immune enhancement, antitumor, antiviral, hypoglycemic, hypolipidemic, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00
Inventor 刘强刘莉
Owner SOUTHERN MEDICAL UNIVERSITY
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