Method for extracting polyoses from bagasse
A technology of bagasse and polysaccharides, which is applied in the field of preparation of polysaccharides, can solve problems such as difficulty in obtaining good results, and achieve the effects of less solvent consumption, high extraction rate, and short extraction time
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Embodiment 1
[0017] Add 1Kg of bagasse coarse powder, add 8 liters of pure ethanol, reflux and extract for 1 hour, filter and collect the filter residue. Add 10 times of water, add 15 grams of cellulase, adjust the pH to 5.5, mix with the filter residue, keep at 60°C for 60 minutes, sonicate for 60 minutes, and filter. The filtrate is a polysaccharide extract. After the extract is concentrated, add ethanol to adjust the alcohol The concentration is 90%, placed overnight, the precipitate is vacuum dried at 60°C, and crushed to obtain 85 g of the crude bagasse polysaccharide.
Embodiment 2
[0019] Add 1Kg of bagasse coarse powder, add 10 liters of 90% ethanol, reflux and extract for 2 hours, and collect the filter residue by filtration. Add 12 times of water, add 5 grams of cellulase, adjust the pH to 4.0, mix with the filter residue, keep at 80°C for 30 minutes, sonicate for 90 minutes, filter, the filtrate is the polysaccharide extract, after the extract is concentrated, add ethanol to adjust the alcohol The concentration is 85%, and it is left overnight, the precipitate is vacuum dried at 70°C, and crushed to obtain 65 g of the crude bagasse polysaccharide.
Embodiment 3
[0021] Add 1Kg of bagasse coarse powder, add 10 liters of 90% ethanol, reflux and extract for 2 hours, and collect the filter residue by filtration. Add 12 times of water, add 10 grams of cellulase, adjust the pH to 6.0, mix with the filter residue, keep at 40°C for 90 minutes, sonicate for 30 minutes, and filter. The filtrate is polysaccharide extract. After the extract is concentrated, add ethanol to adjust the alcohol. The concentration is 85%, the precipitate is left overnight, and the precipitate is vacuum dried at 70° C., and crushed to obtain 64 g of the crude bagasse polysaccharide.
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