Opopanax extract, preparation method and application thereof

A technology of Acacia and its extracts, which is applied in application, animal feed, food preparation, etc., can solve the problems of undisclosed tyrosinase inhibition and undisclosed whitening of Acacia plants, and achieves controllable product quality, Inhibition of melanin production, the method is safe and reliable

Inactive Publication Date: 2014-11-19
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is not disclosed that the composition has a tyrosinase inhibitory effect, nor does it disclose that the Acacia plant has a whitening effect

Method used

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  • Opopanax extract, preparation method and application thereof
  • Opopanax extract, preparation method and application thereof
  • Opopanax extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preferred of embodiment 1 acacia extraction condition

[0030] Fresh Acacia 10g, using L 9 (3 4) orthogonal test (commonly used mathematical methods and computer programs for drug research [P], Guizhou Science and Technology Press, 1997) to optimize the extraction process, with the ratio of solid to liquid (A), ethanol concentration (B), and extraction time (C) as Trial factors, and blank columns (D). Orthogonal design experiments were carried out with the extraction rate as the index to optimize the extraction process conditions. The factor level design is shown in Table 1. Considering the production cost, the extraction time is generally not more than 2 hours. The results are shown in Table 2.

[0031] Table 1 Orthogonal test factor levels

[0032]

[0033] Form 2L 9 (3 4 ) Orthogonal test design and result table

[0034]

[0035] Table 3 Orthogonal test visual analysis table

[0036]

[0037] Table 4 Orthogonal test analysis of variance table

...

Embodiment 2

[0041] The preparation of embodiment 2 Acacia flower bud extract

[0042] After washing 100g of Acacia flower buds, mix them with 2L of 70v / v% ethanol solution, conduct ultrasonic extraction at room temperature for 1 hour, separate the crude extract, evaporate the ethanol solution under reduced pressure, and obtain 6g of Acacia flower bud crude extract.

[0043] Take 1 g of the crude extract of Acacia flower buds, dissolve it in deionized water, load it on the AB-8 resin-filled column, and wash it with deionized water, 20v / v% ethanol, 40v / v% ethanol, 60v / v% ethanol and Gradient elution with 80v / v% ethanol, collect the eluents separately, distill ethanol and water under reduced pressure, and obtain water-washed parts, 20v / v% ethanol parts, 40v / v% ethanol parts, 60v / v% ethanol parts and 80v Acacia flower bud extract from the / v% ethanol fraction, frozen at -20°C.

Embodiment 3

[0044] The preparation of embodiment 3 acacia bark extract

[0045] After washing 50g of Acacia bark, mix it with 1L of 70v / v% ethanol solution, extract it by circulating countercurrent extraction for 2 hours, separate the crude extract, and distill deionized water under reduced pressure to obtain the crude extract of Acacia bark 5g.

[0046] Take 5g of the crude extract of Acacia bark, dissolve it in deionized water, load the sample onto the X-5 resin-filled column, and wash it with deionized water, 20v / v% ethanol, 40v / v% ethanol, and 60v / v% ethanol in sequence and 80v / v% ethanol gradient elution, collect the eluent respectively, evaporate ethanol and water under reduced pressure, and obtain the water washing part, 20v / v% ethanol part, 40v / v% ethanol part, 60v / v% ethanol part and Acacia bark extract in 80v / v% ethanol fraction, frozen at -20°C.

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Abstract

The invention provides an opopanax extract. The extract is prepared by extracting opopanax by a polar solvent, adsorbing the crude extract by virtue of adsorption resin, and eluting by eluent composed of water and ethanol. The opopanax extract is prepared by using a single raw material opopanax, is simple and effective, is capable of inhibiting the activity of tyrosinase, can be applied to cosmetics, and can be used for producing foods, animal feeds, medicines, quasi-drugs and the like.

Description

technical field [0001] The invention relates to a plant extract, in particular to an extract of an acacia plant, a preparation method and its application in cosmetics, or in food, animal feed, medicine and quasi-drug. Background technique [0002] Skin color is one of the criteria of people's aesthetics. For Asian women, fair complexion is a common pursuit. To this end, various products have emerged, such as: whitening products, UV protection products and film products. [0003] After research, skin color is determined comprehensively by various pigments contained in skin cells. Among the many pigments, the amount of melanin contained in melanocytes is the most important determinant. Further studies confirmed that tyrosinase (EC1.14.18.1) is the key enzyme for melanin synthesis. Therefore, by inhibiting the activity of tyrosinase to reduce melanin production, so as to achieve whitening skin care effect has become one of the important basis for research and development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K8/97A23L1/29A23K1/14A61Q19/02A61P39/06A23K10/30A23K40/00A23L33/00
Inventor 方兆华李慧夏增华曹平
Owner JALA GROUP CORPORATION
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