Method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp head

A fatty acid phospholipid, high-purity technology, applied in the direction of chemical instruments and methods, edible phospholipid composition, compounds of group 5/15 elements of the periodic table, etc., can solve the problem of low yield and purity of phospholipids, affecting the quality of phospholipids, and robbing food Resources and other issues, to achieve the effect of wide and cheap raw material sources, reduced extraction costs, and good mechanical strength

Active Publication Date: 2018-06-29
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many technical defects in these methods: raw materials are mainly extracted from soybeans and egg yolks, thereby robbing humans of food resources; supercritical extraction methods, membrane separation methods and other processes are relatively complicated, and the operation cost is high; inorganic salt precipitation method introduces metal Ions (Zn 2+ 、Cd 2+ etc.), have certain toxicity, affect the quality of phospholipids, etc.
[0006] At present, there are few reports on the extraction and preparation methods of phospholipids in shrimp heads. Some researchers used the heads of Penaeus vannamei as raw materials to extract phospholipids with a mixed solvent of n-hexane-isopropanol (Bian Jingjing et al., "Food Science" 2011 Volume 32, Issue 24: 11-15), the yield and purity of phospholipids are low

Method used

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  • Method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp head
  • Method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp head
  • Method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp head

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Weigh 100g of Chinese prawn heads frozen for 20 days, add 100mL of water, pulverize and homogenize for 18min, adjust the pH to 6.5 with acetic acid-sodium acetate buffer solution, heat to 40°C, add 0.2g of compound enzyme, and hydrolyze for about 2h; Centrifuge the enzymolysis solution, pass the solid part through 1000mL 93% ethanol solution (adjust the pH to 9.0 with sodium acetate), ultrasonically extract twice at 34°C, 30min each time, and the ultrasonic frequency is 31KHz, combine the extracts, and filter under reduced pressure , the filtrate was concentrated under reduced pressure at 45°C to obtain crude phospholipids; a small amount of dichloromethane dissolved the crude phospholipids, and after filtration, the filtrate was placed on Bio-Beads S-X 3 Chromatographic column, after cyclohexane elution removes small polar impurities, cyclohexane-ethyl acetate isocratic elution in a ratio of 1:1, the flow rate is 1.0 times the column volume per hour, and is detected by ...

Embodiment 2

[0041] Weigh 100g of fresh vannamei shrimp head, add 150mL of water, pulverize and homogenize for 12min, adjust the pH to 6.5 with acetic acid-sodium acetate buffer solution, heat to 45°C, add 0.4g of compound enzyme, and hydrolyze for about 2h; After centrifugation, the solid part was subjected to 1200mL of 95% ethanol (sodium bicarbonate to adjust its pH to 9.0), ultrasonically extracted twice at 35°C, each time for 15min, and the ultrasonic frequency was 34KHz, the extracts were combined, and after vacuum filtration, the filtrate was Concentrate under reduced pressure at 45°C to obtain crude phospholipids; dissolve the crude phospholipids in a small amount of dichloromethane, filter, and put the filtrate on Bio-Beads S-X 3 Chromatographic column, after cyclohexane elution removes small polar impurities, cyclohexane-ethyl acetate is eluted in a ratio of 3:2, the flow rate is 0.5 times the column volume per hour, and is detected by thin layer chromatography. Dichloromethane-a...

Embodiment 3

[0043] Weigh 100g of fresh Penaeus monodon shrimp head, add 120mL of water, pulverize and homogenize for 8 minutes, adjust the pH to 6.5 with acetic acid-sodium acetate buffer solution, heat to 43°C, add 0.6g of compound enzyme, and hydrolyze for about 1.5h; Solid-liquid separation, the solid part was extracted with 600mL of 90% ethanol (sodium bicarbonate to adjust its pH to 10.0), ultrasonically extracted twice at 32°C, each time for 20min, and the ultrasonic frequency was 32KHz, the combined extracts were filtered under reduced pressure , the filtrate was concentrated under reduced pressure at 43°C to obtain crude phospholipids; a small amount of dichloromethane dissolved the crude phospholipids, and after filtration, the filtrate was placed on Bio-Beads S-X 3 Chromatographic column, after cyclohexane elution removes small polar impurities, cyclohexane-ethyl acetate is eluted at a ratio of 2:1, the flow rate is 1.5 times the column volume per hour, and is detected by thin-la...

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Abstract

The invention provides a method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp heads. The method comprises the steps of homogenizing the shrimp heads, and carrying out enzymolysis by complex enzymes; carrying out solid-liquid separation, adding the solid part into an alkaline ethanol solution, carrying out ultrasonic-assisted extraction, filtering and concentrating to obtain an extract; and dissolving the extract, then carrying out polystyrene gel column chromatography, carrying out thin-layer chromatography detection analysis in combination with an eluent, carrying out reduced pressure condensation, washing by an ice ethanol solution, and then drying to obtain high-purity phospholipids. According to the method, the operation is simple, the extraction process is simple and easy to operate, the fixed adsorption amount of column chromatography fillers is few, repeated use can be achieved, the temperature is not more than 50 DEG C, the physiological activity of phospholipids cannot be damaged, and the purity of the extracted phospholipids is more than 95%. Meanwhile, the invention opens up a new approach for high-valued comprehensive development and utilization of marine product processing wastes, and raw materials are not limited in a certain source and can derive from multiple marine animal processing wastes.

Description

technical field [0001] The invention relates to a method for extracting high-purity omega-3 polyunsaturated fatty acid phospholipids from shrimp heads, and belongs to the technical field of marine active substances. Background technique [0002] Phospholipids are a class of phosphate-containing lipid compounds, which are the basic components of tissue cells, including sphingomyelin and glycerophospholipids. The former is mainly found in the erythrocyte membrane of higher animals, while the latter is abundant in animal liver, brain and ovary. Phospholipids not only have high nutritional value, but also have physiological regulation functions, promote human metabolism, enhance immunity, and prevent diseases. Now, the United States, Europe, Japan and other developed countries have applied phospholipids in clinical practice to prevent brain, heart, liver, tumor and other diseases. In addition, phospholipids also have the characteristics of emulsification, wetting, anti-oxidati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 李晓彬韩利文何秋霞刘可春张姗姗张轩铭侯海荣王雪王希敏张云
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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