Process for extracting coenzyme Q10 from tobacco leaves

A technology for coenzyme and tobacco leaves, which is applied to the technical field of extracting coenzyme Q10, can solve the problems of high cost, high price, and is not suitable for large-scale promotion, and achieves the effects of short time, good effect and improved separation ability.

Inactive Publication Date: 2016-05-11
广西北部湾制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the cost is high, the price is expensive, and it is not suitable for large-scale industrialization

Method used

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  • Process for extracting coenzyme Q10 from tobacco leaves
  • Process for extracting coenzyme Q10 from tobacco leaves

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Experimental program
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Effect test

Embodiment 1

[0026] A process for extracting coenzyme Q10 from tobacco leaves, specifically comprising the steps of:

[0027] 1) Pre-treatment: add the same weight of pure water to the tobacco leaf extract obtained by extracting the waste tobacco leaves, put it into a high-pressure tank, and pressurize it to 4kg / cm 2 , make the pressure uniform, and keep it for 5 minutes, then return to normal pressure within 1-3s, and then use the ethanol solution of NaOH and petroleum ether system to dynamically saponify the bound solanesol of the obtained material, crystallize and filter to obtain the solanesol containing the extract;

[0028] 2) Molecular distillation: the extract containing solanesol obtained in step 1) was removed by molecular distillation with a vacuum degree of 0.01Pa, a distillation temperature of 190°C, a scraper speed of 600r / min, and a material flow rate of 1.0mL / min to obtain Light phase solanesol and heavy phase solanesol;

[0029] 3) Column chromatography separation: the h...

Embodiment 2

[0033]A process for extracting coenzyme Q10 from tobacco leaves, specifically comprising the steps of:

[0034] 1) Pre-treatment: add the same weight of pure water to the tobacco leaf extract obtained by extracting the waste tobacco leaves, put it into a high-pressure tank, and pressurize it to 4kg / cm 2 , make the pressure uniform, and keep it for 10min, then return to the normal pressure within 1-3s, and then use the ethanol solution of NaOH and petroleum ether system to dynamically saponify the bound solanesol of the obtained material, crystallize and filter, and obtain the solanesol containing solanesol the extract;

[0035] 2) Molecular distillation: the extract containing solanesol obtained in step 1) was removed by molecular distillation with a vacuum degree of 0.01Pa, a distillation temperature of 190°C, a scraper speed of 600r / min, and a material flow rate of 1.0mL / min to obtain Light phase solanesol and heavy phase solanesol;

[0036] 3) Column chromatography separa...

Embodiment 3

[0040] A process for extracting coenzyme Q10 from tobacco leaves, specifically comprising the steps of:

[0041] 1) Pre-treatment: add the same weight of pure water to the tobacco leaf extract obtained by extracting the waste tobacco leaves, put it into a high-pressure tank, and pressurize it to 4kg / cm 2 , make the pressure uniform, and keep it for 8 minutes, then return to normal pressure within 1-3s, and then use the ethanol solution of NaOH and petroleum ether system to dynamically saponify the combined solanesol, crystallize and filter the obtained material to obtain the solanesol containing the extract;

[0042] 2) Molecular distillation: the extract containing solanesol obtained in step 1) was removed by molecular distillation with a vacuum degree of 0.01Pa, a distillation temperature of 190°C, a scraper speed of 600r / min, and a material flow rate of 1.0mL / min to obtain Light phase solanesol and heavy phase solanesol;

[0043] 3) Column chromatography separation: the h...

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Abstract

The invention discloses a process for extracting coenzyme Q10 from tobacco leaves. The process comprises the following steps: 1) pretreatment, to be specific, extracting abandoned inferior tobacco leaves to obtain a tobacco leaf extract, adding purified water by equal amount, putting into a high pressure tank, pressurizing to 4kg/cm<2> such that the pressure is uniform, maintaining for 5 to 10min, then recovering to normal pressure within 1 to 3s to obtain a material, and then carrying out saponification treatment on the material, and crystallizing and filtering to obtain a solanesol-containing extract; 2) molecular distillation, to be specific, carrying out molecular distillation on the solanesol-containing extract obtained in the step 1) to remove impurities to obtain light-phase solanesol and heavy-phase solanesol; 3) column chromatography separation, to be specific, dissolving and filtering the heavy-phase solanesol obtained in the step 2) with acetone, carrying out column chromatography separation, collecting eluant and concentrating the eluant to obtain solanesol subjected to molecular distillation and purification; 4) preparation of methyl dimethoxy benzoquinone, to be specific, carrying out brominated etherification and oxidation on methylphenol to obtain the methyl dimethoxy benzoquinone; 5) preparation of the coenzyme Q10 by mixing the light-phase solanesol obtained in the step 2), the solanesol subjected to molecular distillation and purification and obtained in the step 3) and the methyl dimethoxy benzoquinone obtained in the step 4), and reacting.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a process for extracting coenzyme Q10 from tobacco leaves. Background technique [0002] Coenzyme Q10, English name: COENZYMEQ10, CASNo.303-98-0, molecular formula C 59 h 90 o 4 , molecular weight 863.36, yellow to orange crystalline powder, odorless and tasteless, easy to decompose when exposed to light, slightly soluble in ethanol, insoluble in water. Ubiquinone 10, also known as ubiquinone 10, is a fat-soluble quinone whose structure is similar to vitamin K. ring compounds. Coenzyme Q10 is one of the indispensable and important elements of human life. It can activate the nutrition of human cells and cell energy. It has the functions of improving human immunity, enhancing anti-oxidation, delaying aging and enhancing human vitality. It is widely used in the cardiovascular system in medicine. diseases, it is widely used in nutritional health products and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C46/00C07C50/28C07C29/74C07C29/80C07C29/76C07C33/02
CPCC07C46/00C07C29/74C07C29/76C07C29/80C07C50/28C07C33/02
Inventor 劳雪红
Owner 广西北部湾制药股份有限公司
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