Environment-friendly microalgae collecting method

An environmentally friendly, microalgae technology, applied in the field of bioengineering, can solve the problems of high cost of chitosan, low concentration factor of microalgae, unsatisfactory economy, etc. Effect

Inactive Publication Date: 2014-01-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chitosan flocculant is the most representative cationic bioflocculant, which has a good flocculation effect, but the microalgae concentration factor of chitosan flocculation is low, and the cost of chitosan is relatively high, which is not economical. not ideal

Method used

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  • Environment-friendly microalgae collecting method

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Experimental program
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Effect test

Embodiment 1

[0020] Dissolve 1 part of cationic starch in 1000 parts of distilled water, gelatinize in a water bath at 80 °C for 30 min, and cool for 2 h to prepare a cationic starch solution. The microalgae were cultured in the microalgae bioreactor to grow to a concentration of 0.5 g / L, and 80% of the microalgae culture solution was transferred to the flocculation reaction tank through the pipeline, and the substitution degree was added to the microalgae culture solution in the flocculation reaction tank. The 0.15 cationic starch solution was used as a flocculant, so that the dry weight ratio (w / w) of the cationic starch mass to the microalgae in the solution was 0.20, and the microalgae culture solution was stirred at a speed of 200 r / min for 5 minutes to promote the flocculation of the microalgae, and then The microalgae culture solution was left to settle for 10 minutes, the precipitated microalgae concentrate was collected, and the supernatant was circulated for recultivation of the m...

Embodiment 2

[0022] Dissolve 1 part of cationic starch in 1000 parts of distilled water, gelatinize in a water bath at 80 °C for 30 min, and cool for 2 h to prepare a cationic starch solution. The microalgae were cultured in the microalgae bioreactor to grow to a concentration of 0.5 g / L, and 80% of the microalgae culture solution was transferred to the flocculation reaction tank through the pipeline, and the substitution degree was added to the microalgae culture solution in the flocculation reaction tank. The 0.22 cationic starch solution was used as a flocculant, so that the dry weight ratio (w / w) of the cationic starch mass to the microalgae in the solution was 0.15, and the microalgae culture solution was stirred at a speed of 200 r / min for 5 min to promote the flocculation of the microalgae. Then the microalgae culture solution was left to settle for 10 min, the precipitated microalgae concentrate was collected, and the supernatant was recycled for the re-cultivation of the microalgae...

Embodiment 3

[0024] Dissolve 1 part of cationic starch in 1000 parts of distilled water, gelatinize in a water bath at 80 °C for 30 min, and cool for 2 h to prepare a cationic starch solution. The microalgae were cultured in the microalgae bioreactor to grow to a concentration of 0.5 g / L, and 80% of the microalgae culture solution was transferred to the flocculation reaction tank through the pipeline, and the substitution degree was added to the microalgae culture solution in the flocculation reaction tank. The 0.45 cationic starch solution was used as a flocculant, so that the dry weight ratio (w / w) of the cationic starch mass to the microalgae in the solution was 0.08, and the microalgae culture solution was stirred at a speed of 200 r / min for 5 min to promote the flocculation of the microalgae. Then the microalgae culture solution was left to settle for 10 min, the precipitated microalgae concentrate was collected, and the supernatant was recycled for the re-cultivation of the microalgae...

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Abstract

The invention discloses an environment-friendly microalgae collecting method. According to the method, microalgae is cultured into the concentration of 0.5 g/L-1.5g/L in a microalgae bioreactor, 80% of a microalgae culture solution is transferred into a flocculation reaction tank through a pipeline or a pump, a cationic starch solution with a substitution degree of 0.15-0.45 is added into the microalgae culture solution and is taken as a flocculating agent, the wet to dry weight ratio (w/w) of a cationic starch mass to the microalgae in the solution ranges from 0.01 to 0.20, the microalgae culture solution is stirred for 5-10 minutes at the rotation speed of 200 r/min to promote the microalgae to flocculate, the microalgae culture solution is subjected to static settlement for 10-30 minutes, a sedimentary microalgae concentrated solution is collected, and a liquid supernatant is circularly used for re-cultivating the microalgae. According to the environment-friendly microalgae collecting method, the cationic starch solution is taken as the flocculating agent, so that the method has the characteristics of non-toxicity, economy, environmental protection, high flocculation efficiency and the like, flocculated microalgae is dense, the enrichment factors are high, original biological characteristics of the microalgae can be better kept, and the microalgae can be directly used in the fields of food, feed, biodiesel and the like.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to an environment-friendly method for collecting microalgae. Background technique [0002] Microalgae are a type of photosynthetic autotrophic microorganisms, rich in protein, polyunsaturated fatty acids, pigments and other nutrients. At the same time, because of its advantages of fast growth and high yield per unit volume, it has broad application prospects in many fields such as biological feed, biopharmaceuticals, health products, and biofuels. However, it is difficult to harvest because of its extremely small size (3-30 μm) and the low concentration of algae liquid (<1 g / L). Therefore, the efficient harvesting of high-concentration microalgae from algae liquid has become a bottleneck in the development of its industrial scale. [0003] There are a number of problems with currently existing harvesting techniques. The traditional sedimentation method uses the sedime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12
Inventor 林向阳王静李麒龙沈英
Owner FUZHOU UNIV
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