Composition and use thereof, placenta preservation preparation and preparation method thereof

A composition and a technology for placenta, which are applied in the medical field, can solve the problems of placental stem cell toxicity, single nutrient composition, complicated operation and the like, and achieve the effects of good placental mesenchymal cell viability, simple preparation method and simple preparation steps.

Active Publication Date: 2018-02-09
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A variety of antibiotics are added to the existing preservation solution. Although it can reduce the chance of bacterial infection, it has certain toxicity to placental stem cells.
A certain amount of amino acids and human albumin are often added to provide nutrition for the isolated placenta, but the nutritional components are single and the cost is high
At the same time, the existing preservation solution needs to go through steps such as weighing components and sterilizing when preparing, and the operation is cumbersome

Method used

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  • Composition and use thereof, placenta preservation preparation and preparation method thereof
  • Composition and use thereof, placenta preservation preparation and preparation method thereof
  • Composition and use thereof, placenta preservation preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] The preparation of embodiment 1 preservation solution:

[0056] Preparation of sodium citrate buffer solution: Weigh 26.3g of sodium citrate powder, 3.27g of citric acid powder, 12.7g of glucose, 4.44g of sodium dihydrogen phosphate, and 0.55g of adenine, and dissolve the above components in 400mL of In deionized water, stir and mix well to form a 5-fold citrate buffer solution, filter at 0.22 μm, and pack in aliquots.

[0057] Preparation of vitamin C solution: Weigh vitamin C powder, dissolve it in grade I water to form a 0.5 mg / mL vitamin C solution, filter it through 0.22 μm, and dispense it.

[0058] N-acetylcysteine: Weigh N-acetylcysteine ​​powder, dissolve it in grade I water to form a 5mmol / L vitamin C solution, filter it through 0.22μm, and dispense it.

[0059] Cord blood plasma: transfer the cord blood to a 50mL centrifuge tube, seal and centrifuge at 2500rpm for 15min, absorb the upper layer of light yellow plasma into a new centrifuge tube, and store it a...

Embodiment 2

[0061] The preparation of embodiment 2 preservation solution:

[0062] Preparation of sodium citrate buffer solution: Weigh 26.3g of sodium citrate powder, 3.27g of citric acid powder, 12.7g of glucose, 4.44g of sodium dihydrogen phosphate, and 0.55g of adenine, and dissolve the above components in 400mL of In deionized water, stir and mix well to form a 5-fold citrate buffer solution, filter at 0.22 μm, and pack in aliquots.

[0063] Preparation of vitamin C solution: Weigh vitamin C powder, dissolve it in grade I water to form a 0.5 mg / mL vitamin C solution, filter it through 0.22 μm, and dispense it.

[0064] N-acetylcysteine: Weigh N-acetylcysteine ​​powder, dissolve it in grade I water to form a 5mmol / L vitamin C solution, filter it through 0.22μm, and dispense it.

[0065] Cord blood plasma: transfer the cord blood to a 50mL centrifuge tube, seal and centrifuge at 2500rpm for 15min, absorb the upper layer of light yellow plasma into a new centrifuge tube, and store it a...

Embodiment 3

[0067] The preparation of embodiment 3 preservation solution:

[0068] Preparation of sodium citrate buffer solution: Weigh 26.3g of sodium citrate powder, 3.27g of citric acid powder, 12.7g of glucose, 4.44g of sodium dihydrogen phosphate, and 0.55g of adenine, and dissolve the above components in 400mL of In deionized water, stir and mix well to form a 5-fold citrate buffer solution, filter at 0.22 μm, and pack in aliquots.

[0069] Preparation of vitamin C solution: Weigh vitamin C powder, dissolve it in grade I water to form a 0.5 mg / mL vitamin C solution, filter it through 0.22 μm, and dispense it.

[0070] N-acetylcysteine: Weigh N-acetylcysteine ​​powder, dissolve it in grade I water to form a 5mmol / L vitamin C solution, filter it through 0.22μm, and dispense it.

[0071] Cord blood plasma: transfer the cord blood to a 50mL centrifuge tube, seal and centrifuge at 2500rpm for 15min, absorb the upper layer of light yellow plasma into a new centrifuge tube, and store it a...

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Abstract

The invention relates to the field of medicines, in particular to a composition and an application thereof, a placenta preservative and a preparation method of the placenta preservative. Umbilical cord plasma is adopted as a nutrient of a placenta-derived mesenchymal stem cell preservation solution for the first time, so that multi-element nutrient substances are provided for seed cells; the cell viability is maintained; and the original cell morphology and biological characteristics of placenta-derived mesenchymal stem cells can be well kept. Due to the addition of reducing agents such as N-acetylcysteine and vitamin C, and an antioxidant, the resistivity of the placenta-derived mesenchymal stem cells is greatly improved; and the vitality of the placenta-derived mesenchymal stem cells can still be well kept within 24 hours.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a composition and its application, a placenta preservation preparation and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are a research branch of stem cells. Stem cells are a type of cells with self-replication and multi-directional differentiation capabilities. They can continuously renew themselves and transform into one or more types of human tissues or organs under specific conditions Cell. With the maturity of mesenchymal stem cells and related technologies, clinical research has been carried out in many countries. As a seed cell, it is mainly used clinically to treat various intractable diseases of tissue cells and organ damage that cannot be naturally repaired by the body; as an immune regulatory cell, it is used to treat immune rejection and autoimmune diseases. Studies have found that mesenchymal stem cells are mainly derived from bone marro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 王一飞陈海佳葛啸虎卢瑞珊王小燕李平马岩岩
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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