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Serum-free medium for mesenchymal stem cells

A serum-free medium and stromal stem cell technology, applied in the field of mesenchymal stem cell serum-free medium, can solve the problem of lack of mesenchymal stem cell medium and other problems, achieve increased viability, good adherence, and ensure specificity sexual effect

Inactive Publication Date: 2019-01-11
深圳市一五零生命科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a serum-free medium for mesenchymal stem cells, which aims to solve the problem of lacking a medium for large-scale culture and preparation of mesenchymal stem cells in the prior art

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  • Serum-free medium for mesenchymal stem cells
  • Serum-free medium for mesenchymal stem cells
  • Serum-free medium for mesenchymal stem cells

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specific Embodiment

[0040] Serum-free medium for mesenchymal stem cells includes basal medium and supplements. The basic medium is DMEM, and the supplements include amino acids, buffers, albumin, other proteins and nutritional factors.

[0041] Amino acids include L-tyrosine (40mg / L), L-cystine (20mg / L), glycine (10mg / L), L-valine (70mg / L), L-tryptophan (20mg / L L), L-phenylalanine (50mg / L), L-serine (50mg / L), L-threonine (75mg / L), L-alanyl-L-glutamine (500mg / L ) and L-glycyl-L-glutamine (500mg / L).

[0042] Buffers included L-phosphoglycerol disodium salt hydrate (1.5 g / L) and 4-hydroxyethylpiperazineethanesulfonic acid (1.2 g / L).

[0043] Furthermore, the concentration of albumin is: 2g / L.

[0044] Other proteins include Holo Transferrin (human iron transferrin), nutritional factors include Cholesterol (cholesterol), EGF and bFGF.

[0045] The concentrations of these additives are: Holo Transferrin 5mg / L, Cholesterol 0.125mg / L, EGF 0.001mg / L and bFGF 0.001mg / L.

[0046] Prepare the serum-fre...

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Abstract

The invention relates to the technical field of cell culture, and discloses a serum-free medium for mesenchymal stem cells, comprising a basic culture medium and an additive, wherein the additive comprises a serum substitute, other proteins and nutritional factors. The serum-free medium for mesenchymal stem cells has the following advantages: (1) the serum-free culture medium does not contain animal serum or other heterologous proteins, thereby reducing the risk of contamination of the cell preparation; (2), the serum-free culture medium arranged by the invention can reach the imported level in cell culture effect, and the source of the basic culture medium is sufficient and not influenced by the market; (3) Simplify the procedure of cell isolation; the digested cells can be directly usedin primary culture and ensure the specificity of the cultured cells; and (4), using the culture medium of the invention, the cells maintain good adherence, show fusiform fibroblast morphology, and atthe same time, the cells can still maintain a normal state after multiple passages of culture.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a serum-free medium for mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm with self-replication, multilineage differentiation and immune regulation functions. MSCs have the property of low immunogenicity, so they can escape the recognition of T cells. In addition, MSCs can inhibit IL-2-mediated NK cell activation, thereby achieving immune tolerance and avoiding delayed graft-host reactions. MSCs can secrete cytokines, change tissue microenvironment, and promote injury repair. In recent years, MSCs have been widely used clinically, and have significant effects in the treatment of various diseases such as leukemia and other malignant blood diseases, aplastic anemia, refractory immunodeficiency diseases, certain genetic diseases, and repair of tissue and organ damage. curative effect. The mec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N2500/32C12N2500/90C12N2501/998
Inventor 崔兴日吴俊昌苏城昌张捷
Owner 深圳市一五零生命科技有限公司
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