Apparatus and method for measuring contraction force of single platelet in human body

A technology for platelets and contractility, which is applied in measurement devices, biological tests, and material inspection products, etc., can solve the problems of incomplete platelets, long measurement period, and inability to widely use the evaluation of platelet coagulation ability of patients, and achieves simple and convenient construction. the effect of observation

Inactive Publication Date: 2018-08-10
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, it is incomplete to measure the contractility of a single platelet from the contraction force of a blood clot, and the measurement cycle is long, so it cannot be widely used in hospitals to evaluate the coagulation ability of patients' platelets.

Method used

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  • Apparatus and method for measuring contraction force of single platelet in human body
  • Apparatus and method for measuring contraction force of single platelet in human body
  • Apparatus and method for measuring contraction force of single platelet in human body

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Embodiment 1

[0028] A device for measuring the contraction force of a single human platelet, including a three-dimensional platform, a sample pool placed on the three-dimensional platform, a laser detection unit and a lighting unit, the platelet buffer to be detected and simulated fibrinogen are placed in the sample pool, and the lighting unit and the optical path of the laser detection unit as figure 1 As shown, the optical path of the laser detection unit includes laser light source 1, No. 1 reflector 2, beam expander 3, No. 1 dichroic mirror 4, No. 2 reflector 5, objective lens 6, sample cell 7, Condenser 8, No. 2 dichroic mirror 9, No. 1 lens 11 and four-quadrant detector 12, the light path of the illumination unit includes an illumination light source 10, No. 2 dichroic mirror 9, condenser 8, sample pool 7, Objective lens 6, No. 2 reflecting mirror 5, No. 1 dichroic mirror 4, No. 2 lens 13 and CCD camera 14, the sample cell includes a stainless steel support plate and a double-layer g...

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Abstract

The invention relates to an apparatus and method for measuring the contraction force of a single platelet in the human body. The method comprises: forming a surface with silica spheres having the diameter of 100 mum and coated with fibrinogen, capturing a thrombin-activated platelet with optical tweezers, and making the platelet be close to and contact with the silica spheres for interaction, thereby measuring the blood coagulation capability of the single platelet. Compared with a method in the prior art, the method allows the blood coagulation capability of a single platelet to be measured.The method has advantages that a blood sample is convenient to collect, the blood sample is used as soon as the blood sample is collected, the method can be applied in a liquid state, and there is nocontact damage to a biological sample.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a device and method for measuring the contraction force of a single platelet in a human body. Background technique [0002] Platelet (blood platelet, PLT) was discovered and named in 1882 by the Italian physician Bizozero. Platelets in human blood have a diameter of 2 to 3 μm, are small in size, have no nucleus, and are disc-shaped with double-sided dimples. The normal concentration in blood is (100 to 300)×109 / L. Platelets are produced in the cytoplasm of mature megakaryocytes in the bone marrow, and are cleaved and shed from them. They are small pieces of cytoplasm with biological activity and have specific morphological structures and biochemical compositions. It plays an important role in hemostasis, wound healing, angiogenesis, thrombus and organ transplant rejection. [0003] A blood clot formed by the action of platelets and fibrin polymers to prevent the lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49
CPCG01N33/491
Inventor 张鲁凝周娅
Owner TONGJI UNIV
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